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Gastric Cardia Carcinoma is Associated with the Promoter -77T>C Gene Polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1)

  • Original Article
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Journal of Gastrointestinal Surgery Aims and scope

Abstract

Purpose

X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls.

Methods

GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel.

Results

The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1.65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation.

Conclusions

Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

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Acknowledgments

This work was supported by Istituto Toscano Tumori (“Gene expression profile and therapeutic implication in gastric cancer. From the clinical overview to the translational research”; Grant ITT-2007) and by Fundação para a Ciência e a Tecnologia (“Exploring the role of E-Cadherin-HER interaction in the search of molecular biomarkers for the clinical management of gastric cancer patients”; grant ref PIC/IC/82923/2007 and PhD grant ref SFRH/BD/40090/2007).

Competing interests

The author(s) declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Human Pathology and Oncology, Section of General Surgery and Surgical Oncology, Translational Research Laboratory, University of Siena, Siena, Italy

    Giovanni Corso, Daniele Marrelli, Corrado Pedrazzani & Franco Roviello

  2. Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal

    Giovanni Corso, José Carlos Machado & Raquel Seruca

  3. Istituto Toscano Tumori, Florence, Italy

    Giovanni Corso, Daniele Marrelli, Corrado Pedrazzani & Franco Roviello

  4. Faculdade de Medicina da Universidade do Porto, Porto, Portugal

    José Carlos Machado & Raquel Seruca

  5. Department of Surgery, University of Siena, Siena, Italy

    Stefano Mancini

  6. Policlinico le Scotte, Viale Bracci, 53100, Siena, Italy

    Giovanni Corso

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  1. Giovanni Corso
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  2. Daniele Marrelli
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Correspondence to Giovanni Corso.

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Corso, G., Marrelli, D., Pedrazzani, C. et al. Gastric Cardia Carcinoma is Associated with the Promoter -77T>C Gene Polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1) . J Gastrointest Surg 13, 2233–2238 (2009). https://doi.org/10.1007/s11605-009-0980-x

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  • DOI: https://doi.org/10.1007/s11605-009-0980-x

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