Skip to main content
SpringerLink
Log in

Antioxidant activities of crude phlorotannins from Sargassum hemiphyllum

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

Brown algae are well known as a source of biologically active compounds, especially those having antioxidant activities, such as phlorotannins. In this study we examined the antioxidant activities of crude phlorotannins extracts (CPEs) obtained from Sargassum hemiphyllum (SH) and fractionated according to the molecular weights. When CPEs were administrated at a dose of 30 mg/kg to Kunming mice pre-treated with carbon tetrachloride (CCl4), the levels of oxidative stress indicators in the liver, kidney and brain were significantly reduced in vivo. All the components of various molecular weight fractions of CPEs exhibited greater scavenging capacities in clearing hydroxyl free radical and superoxide anion than the positive controls gallic acid, vitamin C and vitamin E. Particularly, the components greater than 30 kD obtained from ethyl acetate phase showed the highest antioxidant capacities. These results indicated that SH is a potential source for extracting phlorotannins, the algal antioxidant compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zou Y, Qian ZJ, Li Y, et al. Antioxidant effects of phlorotannins isolated from CPE igeokamurae in free radical mediated oxidative systems. J Agric Food Chem, 2008, 56(16): 7001–7009

    Article  CAS  PubMed  Google Scholar 

  2. Martínez-Cayuela M. Oxygen free radicals and human disease. Biochimie, 1995, 77(3): 147–161

    Article  PubMed  Google Scholar 

  3. Hensley K, Floyd RA. Reactive oxygen species and protein oxidation in aging: a look back, a look ahead. Arch Biochem Biophys, 2002, 397(2): 377–383

    Article  CAS  PubMed  Google Scholar 

  4. Moskovitz J, Yim MB, Chock PB. Free radicals and disease. Arch Biochem Biophys, 2002, 397: 354–359

    Article  CAS  PubMed  Google Scholar 

  5. Carstro L, Freeman BA. Reactive oxygen species in human health and disease. Nutrition, 2001, 17(2): 161, 163-165

    Article  Google Scholar 

  6. Hofmann T, Liegibel U, Winterhalter P, et al. Intervention with polyphenol-rich fruit juices results in an elevation of glutathione S-transferase P1 (hGSTP1) protein expression in human leucocytes of healthy volunteers. Mol Nutr Food Res, 2006, 5(12): 1191–1200

    Article  Google Scholar 

  7. Shibata T, Kawaguchi S, Hama Y, et al. Local and chemical distribution of phlorotannins in brown algae. J App Phycol, 2004, 16(4): 291–296

    Article  CAS  Google Scholar 

  8. Heo SJ, Park PJ, Park EJ, et al. Antioxidant activity of enzymatic extracts from a brown seaweed Ecklonia cava by electron spin resonance spectrometry and comet assay. Eur Food Res Technol, 2005, 221(1): 41–47

    Article  CAS  Google Scholar 

  9. Sugiura Y, Tanaka R, Katsuzaki H, et al. The antiinflammatory effects of phlorotannins from Eiseniaarboreaon mouse ear edema by inflammatory inducers. J Funct Foods, 2013, 5(4): 2019–2023

    Article  CAS  Google Scholar 

  10. Shim SY, Quang-To L, Lee SH, et al. Ecklonia cava extract suppresses the high-affinity IgE Receptor FceRI expression. Food Chem Toxicol, 2009, 47(3): 555–560

    Article  CAS  PubMed  Google Scholar 

  11. Yoon NY, Chung HY, Kim HR, et al. Acetyl-and butyrylcholinesterase inhibitory activities of sterols and phlorotannins from Ecklonia stolonifera. Fisheries Science, 2008, 74(1): 200–207

    Article  CAS  Google Scholar 

  12. Myung CS, Shin HC, Bao HY, et al. Improvement of memory by dieckol andphlorofucofuroeckol in ethanol-treated mice: possible involvement of the inhibition of acetylcholinesterase. Arch Pharm Res, 2005, 28(6): 691–698

    Article  CAS  PubMed  Google Scholar 

  13. Mattio L, Payri C. 190 years of Sargassum taxonomy, facing the advent of DNA phylogenies. Botanical Rev, 2011, 77(1): 31–70

    Article  Google Scholar 

  14. Liu L, Heinrich M, Myers S, et al. Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine: A phytochemical and pharmacological review. J Ethnopharmacol, 2012, 142(2): 591–619

    Article  PubMed  Google Scholar 

  15. Mao AH, Tang DP, Wang YP, et al. Protective effect of DLE on acute liver injury in mice induced by CCl4. J Anhui AgiSci, 2008, 36(22): 9547–9549

    Google Scholar 

  16. BSISO14502-1: Determination of substances characteristic of green and blacktea. Content of total polyphenols in tea. Colorimetricmethod using Folin-Ciocalteureagent. AW/812/04/2005

  17. Berker K, Güçlü K, Tor I, et al. Total antioxidant capacity assay using optimized ferricyanide/prussian blue method. Food Anal Method, 2010, 3: 154–168

    Article  Google Scholar 

  18. Yu Z, Zhu X, Jiang Y, et al. Bioremediation and fodder potentials of two Sargassum spp. in coastal waters of Shenzhen, South China. Mar Pollut Bull, 2014, 85(2): 797–802

    Article  CAS  PubMed  Google Scholar 

  19. Hwang PA, Chien SY, Chan YL, et al. Inhibition of lippolysacharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264. 7 macrophage cells. J Agric Food Chem, 2011, 59(5): 2062–2068

    Article  CAS  PubMed  Google Scholar 

  20. Wong KH, Cheung PC. Effect of fiber-rich brown seaweeds on protein bioavailability of asein in growing rats. Int J Food Sci Nutr, 2003, 54(4): 269–279

    Article  CAS  PubMed  Google Scholar 

  21. Na HJ, Moon PD, Ko SG, et al. Sargassum hemiphyllum inhibits atopic allergic reaction via the regulation of inflammatory mediators. J PharmacolSci, 2005, 97(2): 219–226

    CAS  Google Scholar 

  22. Lee MS, Shin T, Utsuki T, et al. Isolation and identification of phlarotannins from Eckloniastolonifer with antiodidant and hepatoprotective properties in tacrine-treated HepG2 cells. J Agric Food Chem, 2012, 60(21): 5340–5349

    Article  CAS  PubMed  Google Scholar 

  23. Ganesan K, Kumar KS, Rao PVS. Comparative assessment of antioxidant activity in three edible species of green seaweed, Enteromorpha from Okha, Northwest coast of India. Innova Food Sci Emerging Tech, 2011, 12(1): 73–78

    Article  CAS  Google Scholar 

  24. Venukumar MR, Latha MS. Hepataprotective effect of methanolic extract of Curculigoorchioides in CCl4-treated male rats. Int J Pharmacol, 2002, 34(4): 269–275

    Google Scholar 

  25. Basu S. Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients. Toxicology, 2003, 189(1-2): 113–127

    Article  CAS  PubMed  Google Scholar 

  26. Dolai N, Karmakar I, Kumar RBS, et al. Free radical scavenging activity of Castanopsisindica in mediating hepatoprotective activity of carbon tetrachloride intoxicated rats. Asian Pac J Trop Biomed, 2012, 2(1): 243–251

    Article  Google Scholar 

  27. Li L, Li W, Kim YH, et al. Chlorella vulgaris extract ameliorates carbon tetrachloride-induced acute hepatic injury in mice. Exp Toxicol Pathol, 2013, 65(1-2): 73–80

    Article  CAS  PubMed  Google Scholar 

  28. Kang MC, Kang SM, Ahn G, et al. Protective effect of a marine polyphenol, dieckol against carbon tetrachlorideinduced acute liver damage in mouse. Environ Toxicol Pharmacol, 2013, 35(3): 517–523

    Article  CAS  PubMed  Google Scholar 

  29. Deng JS, Chang YC, Wen CL, et al. Hepatoprotective effect of the ethanol extract of Vitisthunbergiion carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities. J Ethnopharmacol, 2012, 142(3): 795–803

    Article  CAS  PubMed  Google Scholar 

  30. Kang MC, Ahn G, Yang X, et al. Hepatoprotective effects of dieckol-rich phlorotannins from Ecklonia cava, a brown seaweed, against ethanol induced liver damage in BALB/c mice. Food Chem Toxicol, 2012, 50(6): 1986–1991

    Article  CAS  PubMed  Google Scholar 

  31. Abraham P, Wilfred G, Cathrin. Oxidative damage to the lipids and proteins of the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clin Chim Acta, 1999, 289(1-2): 177–179

    Article  CAS  PubMed  Google Scholar 

  32. Balboa EM, Conde E, Moure A, et al. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem, 2013, 138(2-3): 1764–1785

    Article  CAS  PubMed  Google Scholar 

  33. Buonocore G, Perrone S, Tataranno ML. Oxygen toxicity: chemistry and biology of reactive oxygen species. Semin Fetal Neonatal Med, 2010, 15(4): 186–190

    Article  PubMed  Google Scholar 

  34. Nakai M, Kageyama N, Nakahara K, et al. Phlorotannins as radical scavengers from the extract of Sargassumring goldianum. Mar Biotechnol, 2006, 8(4): 409–414

    Article  CAS  PubMed  Google Scholar 

  35. Wang T, Jónsdóttir R, Liu H, et al. Antioxidant capacities of phlorotannins extracted from the brown algae fucusvesiculosus. J Agric Food Chem, 2012, 60(23): 5874–5883

    Article  CAS  PubMed  Google Scholar 

  36. Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial by-products: Anti oxidant activity, occurrence, and potential uses. Food Chem, 2006, 99(1): 191–203

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food and Biology, Guangdong University of Technology, Guangzhou, 510006, China

    Zhi-li Zhao  (赵智丽) & Ya-li Han  (韩雅莉)

  2. School of Pharmacy, Zhanjiang, 524023, China

    Xiao-qing Yang  (杨小青), Zhong-qing Gong  (龚忠勤) & Ming-zhu Pan  (潘明珠)

  3. Key Laboratory of Research & Development of Nature Drug, Guangdong Medical College, Zhanjiang, 524023, China

    Yi Liu  (刘 义)

  4. Shenzhen Enhance Technology Co., Ltd., Shenzhen, 518000, China

    Zhong-qing Gong  (龚忠勤)

  5. People’s Hospital of Longhua District, Shenzhen, 518000, China

    Zhi-li Zhao  (赵智丽)

Authors
  1. Zhi-li Zhao  (赵智丽)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-qing Yang  (杨小青)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhong-qing Gong  (龚忠勤)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ming-zhu Pan  (潘明珠)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ya-li Han  (韩雅莉)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yi Liu  (刘 义)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ya-li Han  (韩雅莉) or Yi Liu  (刘 义).

Additional information

These authors contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, Zl., Yang, Xq., Gong, Zq. et al. Antioxidant activities of crude phlorotannins from Sargassum hemiphyllum . J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 449–455 (2016). https://doi.org/10.1007/s11596-016-1607-6

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-016-1607-6

Key words

Search

Navigation