Skip to main content
SpringerLink
Log in

Expression profile of altered long non-coding RNAs in patients with HBV-associated hepatocellular carcinoma

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

In China, hepatitis B virus (HBV) infection is the major cause of hepatocellular carcinoma (HCC), which is called HBV-related HCC (HBV-HCC), but the pathogenesis has not been clearly elucidated. Long non-coding RNAs (lncRNAs) have been paid increasing attention to, as an important regulatory molecule involved in many biological processes, as well as a variety of diseases. This study examined lncRNA that might play an important role in HBV-HCC pathogenesis by conducting lncRNA and mRNA profile comparison between HBV-HCC and normal liver tissues. The differentially expressed lncRNA and mRNA profiles between HBV-HCC and normal liver tissues were analyzed by microarrays. The potential protein-encoding gene regulated by lncRNA, and the biological function (gene ontology, pathway analysis) of the target gene were investigated. The results showed that the expression levels of lncRNA and mRNA in HBV-HCC tissues were different from those in normal liver tissues. As compared with normal liver tissue, 837 (4.30%) lncRNAs exhibited more than two-fold change (P<0.05); 325 were up-regulated, and 512 were down-regulated; 991 (5.70%) mRNAs exhibited more than 2-fold change (P<0.05); 733 were up-regulated and 258 were down-regulated in HBV-HCC tissue. Besides, there were 7 lncRNAs with above 10-fold elevation, 6 lncRNAs with above 10-fold decrease, 18 mRNAs with above 10-fold elevation and 11 mRNAs with above 10-fold decrease. 444 (53.05%) lncRNAs had their corresponding mRNAs, some of which were adjacent to lncRNAs. The biological analysis showed that the target gene of differentially expressed lncRNAs took part in the important biological regulatory function. Target gene-related pathway analysis revealed the pathways in carcinoma and mitogen-activated protein kinase (MAPK) signaling pathways significantly changed in the HBV-HCC tissues as compared with normal liver tissues (P<0.05). It was suggested that as compared with normal liver tissues, the expression of lncRNAs in HBV-HCC tissues changed significantly, and lncRNAs played a key role in the pathogenesis of HBV-HCC probably by mainly regulating the carcinoma-related signaling pathway and MAPK signaling pathways.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Maluccio M, Covey A. Recent progress in understanding, diagnosing, and treating hepatocellular carcinoma. CA Cancer J Clin, 2012,62(6):394–399

    Article  PubMed  Google Scholar 

  2. Whittaker S, Marais R, Zhu AX. The role of signaling pathways in the development and treatment of hepatocellular carcinoma. Oncogene, 2010,29(36):4989–5005

    Article  PubMed  CAS  Google Scholar 

  3. Farazi PA, DePinho RA. Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer, 2006,6(9):674–687

    Article  PubMed  CAS  Google Scholar 

  4. Brosnan CA, Voinnet O. The long and the short of noncoding RNAs. Curr Opin Cell Biol, 2009,21(3):416–425

    Article  PubMed  CAS  Google Scholar 

  5. Mattick JS, Makunin IV. Non-coding RNA. Hum Mol Genet, 2006,15(Spec 1):R17–29

    Article  PubMed  CAS  Google Scholar 

  6. Chen LL, Carmichael GG. Decoding the function of nuclear long non-coding RNAs. Curr Opin Cell Biol, 2010,22(3):357–364

    Article  PubMed  CAS  Google Scholar 

  7. Ponting CP, Oliver PL, Reik W. Evolution and functions of long noncoding RNAs. Cell, 2009,136(4):629–641

    Article  PubMed  CAS  Google Scholar 

  8. Huarte M, Rinn JL. Large non-coding RNAs: missing links in cancer. Hum Mol Genet, 2010,19 (R2):R152–161

    Article  Google Scholar 

  9. Jia H, Osak M, Bogu GK, et al. Genome-wide computational identification and manual annotation of human long noncoding RNA genes. RNA, 2010,16(8):1478–1487

    Article  PubMed  CAS  Google Scholar 

  10. Tafer H, Hofacker IL. RNAplex: a fast tool for RNA-RNA interaction search. Bioinformatics, 2008,24(22):2657–2663

    Article  PubMed  CAS  Google Scholar 

  11. Panzitt K, Tschernatsch MM, Guelly C, et al. Characterization of HULC, a novel gene with striking up-regulation in hepatocellular carcinoma, as noncoding RNA. Gastroenterology, 2007,132(1):330–342

    Article  PubMed  CAS  Google Scholar 

  12. Ozkan H, Erdal H, Kocak E, et al. Diagnostic and prognostic role of serum glypican 3 in patients with hepatocellular carcinoma. J Clin Lab Anal, 2011,25(5):350–353

    Article  PubMed  Google Scholar 

  13. Ho M, Kim H. Glypican-3: a new target for cancer immunotherapy. Eur J Cancer, 2011,47(3):333–338

    Article  PubMed  CAS  Google Scholar 

  14. Naso MF, Liang B, Huang CC, et al. Dermokine: an extensively differentially spliced gene expressed in epithelial cells. J Invest Dermatol, 2007,127(7):1622–1631

    PubMed  CAS  Google Scholar 

  15. Lee EJ, Jo M, Rho SB, et al. Dkk3, downregulated in cervical cancer, functions as a negative regulator of beta-catenin. Int J Cancer, 2009,124(2):287–297

    Article  PubMed  CAS  Google Scholar 

  16. Yang ZR, Dong WG, Lei XF, et al. Overexpression of Dickkopf-3 induces apoptosis through mitochondrial pathway in human colon cancer. World J Gastroenterol, 2012,18(14):1590–1601

    Article  PubMed  CAS  Google Scholar 

  17. Chen CH, Chuang SM, Yang MF, et al. A novel function of YWHAZ/beta-catenin axis in promoting epithelial-mesenchymal transition and lung cancer metastasis. Mol Cancer Res, 2012,10(10):1319–1331

    Article  PubMed  CAS  Google Scholar 

  18. Saad S, Stanners SR, Yong R, et al. Notch mediated epithelial to mesenchymal transformation is associated with increased expression of the Snail transcription factor. Int J Biochem Cell Biol, 2010,42(7):1115–1122

    Article  PubMed  CAS  Google Scholar 

  19. Chuaysri C, Thuwajit P, Paupairoj A, et al. Alpha-smooth muscle actin-positive fibroblasts promote biliary cell proliferation and correlate with poor survival in cholangiocarcinoma. Oncol Rep, 2009,21(4):957–969

    PubMed  CAS  Google Scholar 

  20. Lee JT. Lessons from X-chromosome inactivation: long ncRNA as guides and tethers to the epigenome. Genes Dev, 2009,23(16):1831–1842

    Article  PubMed  CAS  Google Scholar 

  21. Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions. Nat Rev Genet, 2009,10(3):155–159

    Article  PubMed  CAS  Google Scholar 

  22. Gibb EA, Brown CJ, Lam WL. The functional role of long non-coding RNA in human carcinomas. Mol Cancer, 2011,10:38

    Article  PubMed  CAS  Google Scholar 

  23. Lai JP, Oseini AM, Moser CD, et al. The oncogenic effect of sulfatase 2 in human hepatocellular carcinoma is mediated in part by glypican 3-dependent Wnt activation. Hepatology, 2010,52(5):1680–1689

    Article  PubMed  CAS  Google Scholar 

  24. Khalil AM, Guttman M, Huarte M, et al. Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci USA, 2009,106(28):11 667–11 672

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Infectious Diseases, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China

    Yan-feng Pan  (潘延凤), Lei Feng  (冯 磊) & Zu-jiang Yu  (余祖江)

  2. Department of Hepatobiliary Pancreatic Surgery, the People’s Hospital of Zhengzhou University, Zhengzhou, 450003, China

    Tao Qin  (秦 涛)

Authors
  1. Yan-feng Pan  (潘延凤)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tao Qin  (秦 涛)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lei Feng  (冯 磊)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zu-jiang Yu  (余祖江)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yan-feng Pan  (潘延凤).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pan, Yf., Qin, T., Feng, L. et al. Expression profile of altered long non-coding RNAs in patients with HBV-associated hepatocellular carcinoma. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 96–101 (2013). https://doi.org/10.1007/s11596-013-1078-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-013-1078-y

Key words

Search

Navigation