Summary
The roles of intermediate conductance Ca2+-activated K+ channel (IKCa1) in the pathogenesis of hepatocellular carcinoma (HCC) were investigated. Immunohistochemistry and Western blotting were used to detect the expression of IKCa1 protein in 50 HCC and 20 para-carcinoma tissue samples. Real-time PCR was used to detect the transcription level of IKCa1 mRNA in 13 HCC and 11 para-carcinoma tissue samples. The MTT assay was used to measure the function of IKCa1 in human HCC cell line HepG2 in vitro. TRAM-34, a specific blocker of IKCa1, was used to intervene with the function of IKCa1. As compared with para-carcinoma tissue, an over-expression of IKCa1 protein was detected in HCC tissue samples (P<0.05). The mRNA expression level of IKCa1 in HCC tissues was 2.17 times higher than that in para-carcinoma tissues. The proliferation of HepG2 cells was suppressed by TRAM-34 (0.5, 1.0, 2.0 and 4.0 μmol/L) in vitro (P<0.05). Our results suggested that IKCa1 may play a role in the proliferation of human HCC, and IKCa1 blockers may represent a potential therapeutic strategy for HCC.
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This project was supported by grants from the National Natural Science Foundation of China (No. 81072001) and the Natural Science Foundation of Hubei Province, China (No. 2011CDB556).
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Yang, Xw., Liu, Jw., Zhang, Rc. et al. Inhibitory effects of blockage of intermediate conductance Ca2+-activated K+ channels on proliferation of hepatocellular carcinoma cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 86–89 (2013). https://doi.org/10.1007/s11596-013-1076-0
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DOI: https://doi.org/10.1007/s11596-013-1076-0