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Up-regulation of VLDL receptor expression and its signaling pathway induced by VLDL and β-VLDL

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Summary

Very low density lipoprotein receptor (VLDLR) is thought to participate in the pathogenesis of atherosclerosis induced by VLDL and β-VLDL. The present study was undertaken to elucidate the effects of VLDL and β-VLDL on VLDLR expression and its signaling pathway. RAW264.7 cells were incubated with VLDL and β-VLDL. The expression of VLDLR mRNA was detected by RT-PCR. The transcriptional activity of VLDLR gene was detected in recombinant plasmid pGL4.2VR-luciferase transfected RAW264.7. Western blot assay was used to detect the changes of phosphorylated ERK1/2 protein. Inhibitors or activators were used to observe the signal pathway involving VLDLR expression regulation. The results showed that VLDL and β-VLDL stimulated ERK1/2 activity in a PKC-dependent manner. VLDL or β-VLDL-induced VLDLR expression on macrophages was extremely abolished by inhibitors ERK1/2 or PKC. Our findings revealed that VLDL or β-VLDL-induced VLDLR expression via PKC/ERK cascades and the effect was linked to the transcriptional activation of VLDLR gene promoter.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhiguo Liu  (刘志国), He Li  (李 和), Yinghong Li  (李映红), Yan Wang  (王 燕), Yiqiang Zong  (宗义强), Youmei Feng  (冯友梅), Zongchen Feng  (冯宗忱), Yaozu Deng  (邓耀祖) & Shen Qu  (屈 伸)

  2. Department of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

    Zhiguo Liu  (刘志国)

Authors
  1. Zhiguo Liu  (刘志国)
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  2. He Li  (李 和)
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  3. Yinghong Li  (李映红)
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  4. Yan Wang  (王 燕)
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  5. Yiqiang Zong  (宗义强)
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  6. Youmei Feng  (冯友梅)
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  7. Zongchen Feng  (冯宗忱)
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  8. Yaozu Deng  (邓耀祖)
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  9. Shen Qu  (屈 伸)
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Additional information

This project was supported by grant from National Natural Sciences Foundation of China (No. 39970307) and Hubei Provincial Natural Sciences Foundation of China (No. 2005ABA092).

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Liu, Z., Li, H., Li, Y. et al. Up-regulation of VLDL receptor expression and its signaling pathway induced by VLDL and β-VLDL. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 1–7 (2009). https://doi.org/10.1007/s11596-009-0101-9

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  • DOI: https://doi.org/10.1007/s11596-009-0101-9

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