Skip to main content
SpringerLink
Log in

Bone formation of embryonic stem cell-derived mesenchymal stem cells

  • Original Article
  • Cell Biology
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Human embryonic stem cells are multipotent cells. In this study, We observed osteogenesis of human embryonic stem cell derived mesenchymal stem cells. mbryonic body formation method was used to derive mesenchymal stem cells from human embryonic stem cells. Embryonic stem cell derived mesenchymal stem cells were immunostained for CD 73 to make characterization of mesencymal stem cells. Osteogenesis of CD 73 positive mesencymal stem cells with media included ascorbic acid, dexamethasone and glycerophosphate was induced. After 10 days culture with osteogenesis media, the cells were immunostained with type I collagen, osteocalcin and Runx-2. After 21 days culture with osteogenesis media, the cells were stained with alizarin red to observe bone nodule formation. Mesenchymal stem cells were derived from human embryonic stem cells by embryonic body method. After these cells were cultured with osteogenesis media, the cells were differentiated into osteoblast and showed bone nodule formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145–1147.

    Article  CAS  PubMed  Google Scholar 

  2. Smith AGG. Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 2001;17:435–462.

    Article  CAS  PubMed  Google Scholar 

  3. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–147.

    Article  CAS  PubMed  Google Scholar 

  4. Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, et al. Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell 2009; 4:381–384.

    Article  CAS  PubMed  Google Scholar 

  5. Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 2009;4:472–476.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Hu J, Smith LA, Feng K, Liu X, Sun H, Ma PXX. Response of human embryonic stem cell-derived mesenchymal stem cells to osteogenic factors and architectures of materials during in vitro osteogenesis. Tissue Eng Part A 2010;16: 3507–3514.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Wang S, Qu X, Zhao RCC. Mesenchymal stem cells hold promise for regenerative medicine. Front Med 2011; 5:372–378.

    Article  PubMed  Google Scholar 

  8. Heng BC, Cao T, Stanton LW, Robson P, Olsen B. Strategies for directing the differentiation of stem cells into the osteogenic lineage in vitro. J Bone Miner Res 2004;19:1379–1394.

    Article  CAS  PubMed  Google Scholar 

  9. Gamie Z, Tran GT, Vyzas G, Korres N, Heliotis M, Mantalaris A. Stem cells combined with bone graft substitutes in skeletal tissue engineering. Expert Opin Biol Ther 2012;12:713–729.

    Article  CAS  PubMed  Google Scholar 

  10. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. Cytotherapy 2006;8:315–317.

    Article  CAS  PubMed  Google Scholar 

  11. Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 2008;6:88–95.

    Google Scholar 

  12. Varga N, Veréb Z, Rajnavölgyi E, Német K, Uher F, Sarkadi B, et al. Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth. Biochem Biophys Res Commun 2011;414:474–480.

    Article  CAS  PubMed  Google Scholar 

  13. Sottile V, Thomson A, McWhir J. In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 2003;5:149–155.

    Article  CAS  PubMed  Google Scholar 

  14. Barberi T, Willis LM, Socci ND, Studer L. Derivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med 2005;2:554–560.

    Article  CAS  Google Scholar 

  15. Olivier EN, Rybicki AC, Bouhassira EEE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 2006; 24:1914–1922.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Anatomy, Dental School, Gangneung Wonju National University, 7 Jukheon-gil, Gangneung, 210-702, Korea

    Yeon Tae Jung, Ki-Yeon Yoo & Hee Su Lee

Authors
  1. Yeon Tae Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ki-Yeon Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee Su Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hee Su Lee.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jung, Y.T., Yoo, KY. & Lee, H.S. Bone formation of embryonic stem cell-derived mesenchymal stem cells. Tissue Eng Regen Med 12 (Suppl 2), 132–137 (2015). https://doi.org/10.1007/s13770-014-0425-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13770-014-0425-9

Key Words

Search

Navigation