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Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat

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Summary

To investigate the technique of sorting high-purity precartilaginous stem cells from rat’s perichondrium, neonatal rat’s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0 %–99.0 %, with living cells amounting to 80 %–85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.

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Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huangzhong University of Science & Technology, China

    Cheng Hao M. D., Ph. D.  (程浩) (male), Chen Anmin  (陈安民) & You Hongbo  (游洪波)

Authors
  1. Cheng Hao M. D., Ph. D.  (程浩)
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  2. Chen Anmin  (陈安民)
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  3. You Hongbo  (游洪波)
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Additional information

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30170944).

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Cheng, H., Chen, A. & You, H. Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat. J. Huazhong Univ. Sc. Technol. 26, 723–724 (2006). https://doi.org/10.1007/s11596-006-0626-0

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  • DOI: https://doi.org/10.1007/s11596-006-0626-0

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