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Assessing the Bone-Forming Potential of Pericytes

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Pericytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2235))

Abstract

Human pericytes are a perivascular cell population with mesenchymal stem cell properties, present in all vascularized tissues. Human pericytes have a distinct immunoprofile, which may be leveraged for purposes of cell purification. Adipose tissue is the most commonly used cell source for human pericyte derivation. Pericytes can be isolated by FACS (fluorescence-activated cell sorting), most commonly procured from liposuction aspirates. Pericytes have clonal multilineage differentiation potential, and their potential utility for bone regeneration has been described across multiple animal models. The following review will discuss in vivo methods for assessing the bone-forming potential of purified pericytes. Potential models include (1) mouse intramuscular implantation, (2) mouse calvarial defect implantation, and (3) rat spinal fusion models. In addition, the presented surgical protocols may be used for the in vivo analysis of other osteoprogenitor cell types.

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Acknowledgments

A.W.J. was supported by the NIH/NIAMS (R01 AR070773, K08 AR068316), NIH/NIDCR (R21 DE027922), Department of Defense (W81XWH-18-1-0121, W81XWH-18-1-0336, W81XWH-18-10613), American Cancer Society (Research Scholar Grant, RSG-18-027-01-CSM), the Maryland Stem Cell Research Foundation, MTF Biologics, and Novadip. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, Department of Defense, or U.S. Army.

Disclosures: K.T, B.P, and C.S. are inventors of PSC-related patents filed from UCLA. A.W.J. is a paid consultant for Novadip. This arrangement has been reviewed and approved by the Johns Hopkins University in accordance with its conflict of interest policies.

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Authors and Affiliations

  1. Department of Pathology, Johns Hopkins University, Baltimore, MD, USA

    Carolyn A. Meyers, Jiajia Xu & Aaron W. James

  2. Orthopedic Hospital Research Center and David Geffen School of Medicine, University of California, Los Angeles, CA, USA

    Chenchao Wang, Chia Soo, Bruno M. Péault & Aaron W. James

  3. School of Dentistry, University of California, Los Angeles, CA, USA

    Chenchao Wang, Hsin Chuan Pan, Jia Shen & Kang Ting

  4. Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China

    Chenchao Wang

  5. Department of Surgery, University of California, Los Angeles, CA, USA

    Chia Soo

  6. BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK

    Bruno M. Péault

Authors
  1. Carolyn A. Meyers
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  2. Chenchao Wang
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  3. Jiajia Xu
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  4. Hsin Chuan Pan
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  5. Jia Shen
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  6. Kang Ting
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  7. Chia Soo
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  8. Bruno M. Péault
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  9. Aaron W. James
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Corresponding author

Correspondence to Aaron W. James .

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Editors and Affiliations

  1. Orthopedic Hospital Research Center and David Geffen School of Medicine, University of California, Los Angeles, CA, USA, BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK

    Bruno M. Péault

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Meyers, C.A. et al. (2021). Assessing the Bone-Forming Potential of Pericytes. In: Péault, B.M. (eds) Pericytes. Methods in Molecular Biology, vol 2235. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1056-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1056-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1055-8

  • Online ISBN: 978-1-0716-1056-5

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