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Preparation and Evaluation of MPEG-PCL Polymeric Nanoparticles Against Gastric Cancer

  • Biomaterial
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Abstract

This paper provides a new treatment for gastric cancer with a new OMT delivery system. We synthesized MPEG-PCL, an amphiphilic polymer, to construct a nanoparticle encapsulated OMT by pH gradient method, and then examined the nanodrug’s therapeutic efficacy. An integral analytical method was used to characterize the structure of MPEG-PCL. The single factor method and orthogonal test were utilized to investigate the optimum preparation process. The morphology and average size of the OMT-NPs were analyzed by transmission electron microscopy and Zetasizer. CCK-8 assay and confocal fluorescent microscope were used to study the inhibitory effect on SGC-7901 gastric cancer cells. The average size of nanoparticles was 95.86±1.54 nm. The maximum encapsulation efficiency of OMT was 46.84±4.37%, while the drug loading content was 8.89±1.09%. The cumulative release of nanoparticles was 73.07±1.5%, inspected through dynamic dialysis in vitro. Compared with free OMT, OMT-NPs showed enhanced cytotoxic effects in SGC-7901 cells. The nanoparticles could efficiently deliver the OMT into the cancer cells and release it. The OMT delivery system prepared in this paper provides a potential platform for the treatment of gastric cancer.

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Authors and Affiliations

  1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of technology, Wuhan, 430070, China

    Xia Liu  (刘霞), Juncan Li, Yaowen Wang, Mengjia Yang & Mingxiu Tang  (汤明秀)

  2. China Tobacco Hubei Industrial Co., Ltd, Wuhan, 430000, China

    Long Huang

  3. College of Chemical and Environmental Engineering, Hubei University for Nationalities, Enshi, 445000, China

    Jin Yang

  4. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China

    Tong Qiu  (邱彤)

  5. Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan, 430070, China

    Tong Qiu  (邱彤)

Authors
  1. Xia Liu  (刘霞)
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  2. Juncan Li
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  3. Long Huang
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  4. Jin Yang
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  5. Yaowen Wang
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  6. Mengjia Yang
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  7. Mingxiu Tang  (汤明秀)
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  8. Tong Qiu  (邱彤)
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Corresponding authors

Correspondence to Mingxiu Tang  (汤明秀) or Tong Qiu  (邱彤).

Additional information

Funded by the National Natural Science Foundation of China (No. 51273156) and the Health Commission of Hubei Province Scientific Research Project (No. WJ2019H275)

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Liu, X., Li, J., Huang, L. et al. Preparation and Evaluation of MPEG-PCL Polymeric Nanoparticles Against Gastric Cancer. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 35, 1162–1168 (2020). https://doi.org/10.1007/s11595-020-2368-4

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  • DOI: https://doi.org/10.1007/s11595-020-2368-4

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