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Evaluation of PLGA/chitosan/HA conduits for nerve tissue reconstruction

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Abstract

A micro-envioment for nerve cells and tissue growth were designed and constructed via surface modification of poly(L-lactide-co-glycolide)(PLGA) with chitosan and hydroxyapatite(HA). The poly(L-lactide-co-glycolide)/chitosan/hydroxyapatite (PLGA/chitosan/HA) conduits were manufactured by a combined solvent casting and particulate leaching technique. The conduits were highly porous with an interconnected pore structure and 76.5% porosity. Micropores with 50–100 micrometer diameter were formed in the conduits. In vivo application of PLGA/chitosan/HA conduits for reconstruction of 10 mm sciatic nerve defect was assessed by the walking track analysis, the quantifying of the wet weight of tibialis anterior muscle and the histological assessment. The conduits in host rats in vivo can not only be an effective in promoting regenerating of nerves but can also lead to favorable nerve functional recovery.

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Authors and Affiliations

  1. State Key Laboratory of Powder Metallurgy, Central South University, Changsha, 41008, China

    Xiongjun Shen  (申雄军), Jianming Ruan  (阮建明) & Zhongchi Zhou

  2. College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, 410076, China

    Xiongjun Shen  (申雄军)

  3. College of Basic Medicine, Central South University, Changsha, 410083, China

    Zhicheng Zeng & Lesi Xie

Authors
  1. Xiongjun Shen  (申雄军)
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  2. Jianming Ruan  (阮建明)
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  3. Zhongchi Zhou
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  4. Zhicheng Zeng
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  5. Lesi Xie
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Corresponding author

Correspondence to Jianming Ruan  (阮建明).

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Funded by the National Natural Science Foundation of China(No.50774096)

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Shen, X., Ruan, J., Zhou, Z. et al. Evaluation of PLGA/chitosan/HA conduits for nerve tissue reconstruction. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 24, 566–570 (2009). https://doi.org/10.1007/s11595-009-4566-y

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  • DOI: https://doi.org/10.1007/s11595-009-4566-y

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