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A novel electrochemical method for ofloxacin determination based on interaction of ofloxacin with cupric ion

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Abstract

An indirect electrochemical sensing strategy for the determination of ofloxacin (OFL) was developed using cupric ion (Cu2+) as an electrochemical probe. The method was based on the complexation of OFL with Cu2+, which was investigated by UV-visible spectrophotometry and differential pulse voltammetry (DPV). When OFL was added into the electrolyte solution containing Cu2+, the electro-reduction peak current of Cu2+ on glassy carbon electrode (GCE) was decreased. Some influencing factors in terms of pH, quiet time, and reaction time were systematically studied. Under optimal conditions, the Cu2+ reduction peak current difference (ΔI p) before and after adding OFL was found to be linear to the concentration of OFL in the range from 1.0 × 10−7 to 1.0 × 10−4 M. The detection limit (3S/N) was 8.2 × 10−8 M. Moreover, the proposed sensor displayed high selectivity and good reproducibility, which was successfully applied to the detection of OFL in pharmaceutical tablet and chicken fodder.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 61172005) and the National High Technology Research and Development Program of China (863 Program) (Grant No. 2012AA06A304).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Ruizhen Li, Songsong Lv, Jun Shan & Jingdong Zhang

  2. College of Materials and Chemical Engineering, Sichuan University of Science and Engineering, Zigong, Sichuan, 643000, People’s Republic of China

    Ruizhen Li

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  1. Ruizhen Li
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Correspondence to Jingdong Zhang.

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Li, R., Lv, S., Shan, J. et al. A novel electrochemical method for ofloxacin determination based on interaction of ofloxacin with cupric ion. Ionics 21, 3117–3124 (2015). https://doi.org/10.1007/s11581-015-1492-1

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