Abstract
The effects of heavy metals Cd2+, Pb2+ and Zn2+ at 0.05, 0.5 and 5.0 mg/L level and their interactions at 0.5 mg/L level on DNA damage in hepatopancreas of loach Misgurnus anguillicaudatus for 1–35 days exposure were examined by single cell gel electrophoresis (SCGE). For each test group, 20 loaches with similar body size (5.17–7.99 g; 11.79–13.21 cm) were selected and kept in aquaria with dechlorinated water at (22±1)°C and fed a commercial diet every 48 h. According to the percentage of damaged DNA with tail and its TL/D (tail length to diameter of nucleus) value, the relationship between DNA damage degree and heavy metal dose and exposure time was determined. Results showed that the percentage of damaged DNA and the TL/D value were increased with the prolonged exposure time. The highest percentage (84.85%) of damaged DNA was shown in 5.0 mg/L Zn2+ group after 28 days exposure and the biggest TL/D value (2.50) in all treated groups after 35 days exposure. During the first treated week, the damnification of DNA was mainly recognized as the first level, after that time, the third damaged level was mostly observed and the percentage of damaged DNA was beyond 80%. The joint toxic effects among Cd2+, Pb2+ or Zn2+ revealed much complexity, but it generally displayed that the presence of Cd2+ could enhance the genotoxicity of Pb2+ or Zn2+. In conclusion, the results suggested that there was a significant time-and dose-depended relationship between the heavy metal and DNA damage in hepatopancreas of loach, and SCGE could represent a useful means to evaluate the genotoxicity of environmental contamination on aquatic organisms.
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References
Feng S L, Kong Z M, Wang W X, Wang X M, Peng P A (2004). Genotoxic studies of pesticides to tadpoles and frogs of Rana nigrinaculata Halloweii by micronuclei test and single cell gel electrophoresis assay. Acta Hydrobiological Sinica, 28: 52–57 (in Chinese)
Gabbianelli R, Lupidi G, Villarini M, Falcioni G (2003). DNA damage induced by copper on erythrocytes of gilthread sea bream spaus aurata and mollusk scapharca inaequivalvis. Archives Environmental Contamination and Toxicology, 45: 350–356
Hu X P, Shi X J, Zhou J H (2005a). Study of DNA damage in Carassius auratus lymphocytes treated by mixed heavy metals. Reservoir Fisheries, 25: 11–12 (in Chinese)
Hu X P, Zhou J H, Shi X J (2005b). Detection of DNA damage in Carassius auratus lymphocytes induced by cadmium with single cell gelelectrophoresis assay. Journal of Agro-Environment Science, 24: 43–45 (in Chinese)
Jia X Y (2001). Effects of four kinds of heavy metal on respiration intensity of juvenile Misgurnus anguillicaudatus. Journal of Zhejiang University (Agric and Life Sci), 27: 556–558 (in Chinese)
Jia X Y (2001). Acute and subacute toxicity of cadmium on juvenile loach. Environmental Pollution and Control, 23: 227–228 (in Chinese)
Li X N, Han Y L, Deng R P, Ao L M, Zhou X P (2005). Effects of tributyltin on DNA damage of gill cell from Saccostresa cucullata. Acta Hydrobiological Sinica, 29: 336–339 (in Chinese)
Ma H M (2002). Law of variety and straits of the pollutants in the Lanzhou strain of the Yellow River. Gansu Environmental Study and Monitoring, 15: 101–103 (in Chinese)
Pandrangi R, Petras M, Rasph S (1995). Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp. Environmental Molecular Mutagen, 96: 345–356
Sun D W, Zhan Y, Xu Z R (2003). Study on the hazards of heavy metals to fishes. Reservoir Fisheries, 23: 4–6 (in Chinese)
Xie Z H, Li J, Ping B, Fan Y Q (2003). Study on genetic toxicity of four herbicides on erythrocytes of Misgurnus anguillicaudatus. Chinese Journal Applied Environmental Biology, 9: 412–416 (in Chinese)
Yang J Y, Pen Y, Li L (2002). Inspection of the destruction of single-cell DNA using SCGE. Shanxi Medical Journal, 31: 400–402 (in Chinese)
Yang J, Yin J, Xu H L, Gong X Q, Yu R P, Wang D (2005). The ingestion and accumulation of zinc, copper, lead, cadmium and arsenic in the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. Acta Hydrobiological Sinica, 29: 557–563 (in Chinese)
Zhao S C, Zhang J (1999). Study on the effects of chromium (Cr3+) on DNA damaging in carp (Carassius auratus) kidney cells. Hebei Fisheries, 4: 11–13 (in Chinese)
Zhou X W, Zhu G N, Sun J H, Ge C L (2001). Toxicity of copper, zinc, lead, cadmium to tissue’s cellular DNA of the fish (Carassius auratus). Acta Agricultural Nuclearae Sinica, 15: 167–173 (in Chinese)
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Translated from Acta Hydrobiologica Sinica, 2006, 30(4): 399–403 [译自: 水生生物学报]
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Zhang, Y., Wang, Y., Yu, R. et al. Effects of heavy metals Cd2+, Pb2+ and Zn2+ on DNA damage of loach Misgurnus anguillicaudatus . Front. Biol. China 3, 50–54 (2008). https://doi.org/10.1007/s11515-008-0012-3
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DOI: https://doi.org/10.1007/s11515-008-0012-3