Abstract
Dehydration-responsive element-binding (DREB) proteins, specifically binding to the dehydration-responsive element (DRE), have been identified as a group of important transcription activators of plants which regulate the expression of genes in response to drought, high-salt and low-temperature stresses. Two DREB-like genes from Bermuda grass that are induced by low-temperature or high-salt stresses were cloned using RT-PCR and RACE methods, and were named BeDREB1 and BeDREB2, respectively (GenBank accession No: AY462117 and AY462118). They contained an ORF of 753 bp encoding 251 amino acids, showing the typical characteristics of the DREB gene family. Interestingly, these two genes isolated from Bermuda grass induced either by low-temperature stress or high-salt stress shared 97.8% homology. Furthermore, it was demonstrated that both BeDREB1 and BeDREB2 could bind to the wild-type DRE element to activate the transcription of the reporter gene HIS3, driven by a promoter carrying DRE cis-element in yeast strain 4721, in the presence of 3-AT. RT-PCR showed that BeDREB1 and BeDREB2 genes could be greatly induced by low-temperature and high-salt stresses, respectively. Their expressions were changed following the inducible time. In conclusion, all results indicate that BeDREB1 and BeDREB2 genes isolated from treated Bermuda grass are new members of the DREB transcription activator family, which may play very important roles in signal transduction related to stresses.
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References
Cao Z. F., Li J., Chen F., Li Y. Q., Zhou H. M., Liu Q., Effect of two conserved amino acid residues on DREB1A function. Biochemistry, 2001, 66(6):623–627
Gilmour S. J., Zarka D. G., Stockinger E. J., Salazar M. P., Houghton J. M., Thomashow M. F., Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J., 1998, 16(4): 433–442
Haake V., Cook D., Riechmann J. L., Pineda O., Thomashow M. F., Zhang J. Z., Transcription factors CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol., 2002, 130(2): 639–648
Hong J. P., Kim W. T., Isolation and functional characterization of the Ca-DREBLP1 gene encoding a dehydration-responsive element binding-factor-like protein 1 in hot pepper (Capsicum annuum L. cv. Pukang). Planta., 2005, 220(6): 875–888
Kizis D., Lumbreras V., Pages M., Role of AP2/EREBP transcription factors in gene regulation during abiotic stress. FEBS Lett., 2000, 498(2–3): 187–189
Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K., Shinozaki K., Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA-binding domain separate two cellular signal transduction pathways in drought-and low-temperature responsive gene expression in Arabidopsis. Plant Cell, 1998, 10(8): 1391–1406
Liu Q., Zhao N. M., Yamaguch-Shinozaki K., Shinozaki K., Regulatory role of DREB transcription factors in plant drought, salt and cold-tolerance. Chin. Sci. Bull., 2000, 45(1): 11–16 [刘强, 赵南明, Yamaguch-Shinozaki K., Shinozaki K., DREB 转录因子在提高植物抗逆性中的作用. 科学通报, 2000, 45(1): 11–16]
Medina J., Bargues M., Terol J., Terol J., Alonso M. P., Salinas J., The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol., 1999, 119(2): 463–469
Nakashima K., Shinwari Z. K., Sakuma Y., Seki M., Miura S., Shinozaki K., Yamaguchi-Shinozaki K., Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration-and high-salinity-responsive gene expression. Plant Mol. Biol., 2000, 42(4): 657–665
Qin F., Li J., Zhang G. Y., Zhao J., Chen S. Y., Liu Q., Isolation and structural analysis of DRE-binding transcription factor from Maize (Zea mays L.). Acta Botanica Sinica, 2003, 45(3): 331–339
Riechmann J. L., Heard J., Martin G., Teuber L., Jiang C., Keddie J., Adam L., Pieneda O., Tatcliffe O. J., Samaha R. R., Creelman R., Pilgrim M., Broun P., Zhang J. Z., Ghandehair D., Sherman B. K., Yu G., Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000, 290(5499): 2105–2110
Riechmann J. L. and Meyerowitz E. M., The AP2/EREBP family of plant transcription factors. Biol. Chem., 1998, 379(6): 633–646
Sakuma Y., Liu Q., Dubouzet J. G., Abe H., Shinozaki K., Yamaguchi-Shinozaki K., DNA-Binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration-and cold-inducible gene expression. Biochem. Biophyl. Res. Co., 2002, 290(3): 998–1009
Shen Y. G., Zhang W. K., He J. S., A AP2/EREBP type protein in Triticum aestivum was a DRE-transcription factors induced by cold, dehydration and ABA stress. Theor. Appl. Genet., 2003, 106(5): 923–930
Shinozaki K. and Yamaguchi-Shinozaki K., Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr. Opin. Plant Biol., 2000, 3(3): 217–223
Shinozaki K and Yamaguchi-Shinozaki K., Molecular responses to drought and cold stress. Curr. Opin. Biotech., 1996, 7(2): 161–167
Thomashow M. F., Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Physiol. Plant Mol. Bio., 1999, 50: 571–599
Xue G. P. and Loveridge C. W., HvDRF1 is involved in abscisic acid-mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT-rich element. Plant J., 2004, 37(3): 326–339
Yamaguchi-Shinozaki K., Koizumi M., Urao S., Shinozaki K., Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: Sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant Cell Physiol., 1992, 33: 217–224
Yamaguchi-Shinozaki K. and Shinozaki K., A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 1994, 6(2): 251–264
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Translated from Chinese Journal of Biochemistry and Molecular Biology, 2005, 21(4): 521–527 [译自: 中国生物化学与分子生物学报, 2005, 21(4): 521–527]
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Xie, Y., Wang, Z., Liu, Q. et al. Cloning and functional identification of stress-resistant BeDREB genes from Bermuda grass. Front. Biol. China 1, 367–374 (2006). https://doi.org/10.1007/s11515-006-0048-1
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DOI: https://doi.org/10.1007/s11515-006-0048-1