Skip to main content
SpringerLink
Log in

Exploring Metabolic Responses of Potato Tissue Induced by Electric Pulses

  • Original Article
  • Published:
Food Biophysics Aims and scope Submit manuscript

Abstract

In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. J. Teissié, M. Golzio, M.P. Rols, Biochim. Biophys. Acta (G) 1724, 270 (2005)

    Google Scholar 

  2. M.M. Góngora-Nieto, P.D. Pedrow, B.G. Swanson, G.B. Barbosa-Canovas, J. Food Eng. 57, 209 (2003)

    Article  Google Scholar 

  3. S. Toepfl, V. Heinz, D. Knorr, Chem. Eng. Process. 46, 537 (2007)

    Article  CAS  Google Scholar 

  4. M. Fincan, F. DeVito, P. Dejmek, J. Food Eng. 64, 381 (2004)

    Article  Google Scholar 

  5. Y. Chalermchat, P. Dejmek, J. Food Eng. 71, 164 (2005)

    Article  Google Scholar 

  6. B.I.O. Ade-Omowaye, N.K. Rastogi, A. Angersbach, D. Knorr, J. Food Eng. 60, 89 (2003)

    Article  Google Scholar 

  7. N.I. Lebovka, N.V. Shynkaryk, E. Vorobiev, J. Food Eng. 78, 606 (2007)

    Article  Google Scholar 

  8. B. Gabriel, J. Teissié, Eur. J. Biochem. 223, 25 (1994)

    Article  CAS  Google Scholar 

  9. N. Sabri, B. Pelissier, J. Teissié, Eur. J. Biochem. 238, 737 (1996)

    Article  CAS  Google Scholar 

  10. H. Ye, L.L. Huang, S.D. Chen, J.J. Zhong, Biotechnol. Bioeng. 88, 788 (2004)

    Article  CAS  Google Scholar 

  11. M. Guderjan, P. Elez-Martinez, D. Knorr, Innov. Food Sci. Emerg. Technol. 8, 55 (2007)

    Article  CAS  Google Scholar 

  12. M. Guderjan, S. Topfl, A. Angersbach, D. Knorr, J. Food Eng. 67, 281 (2005)

    Article  Google Scholar 

  13. F. Gómez Galindo, I. Sjöholm, A.G. Rasmusson, S. Widell, K. Kaack, Crit. Rev. Food Sci. Nutr. 47, 729 (2007)

    Article  CAS  Google Scholar 

  14. C.J. Atkinson, R. Nestby, Y.Y. Ford, P.A.A. Dodds, Biofactors 23, 229 (2005)

    CAS  Google Scholar 

  15. M. Schreiner, S. Huyskens-Keil, Crit. Rev. Plant Sci. 25, 267 (2006)

    Article  CAS  Google Scholar 

  16. R.S. Criddle, R.W. Breindenbach, L.D. Hansen, Thermochim. Acta 193, 67 (1991)

    Article  CAS  Google Scholar 

  17. K. Kinosita, T.Y. Tsong, Biochim. Biophys. Acta 471, 227 (1977)

    Article  CAS  Google Scholar 

  18. Y. Chalermchat. Effects of Pulsed Electric Fields on Plant Tissue, PhD Thesis. Lund University, Sweden (2005)

  19. P. Rocculi, F. Gómez Galindo, F. Mendoza et al., Post Biol Tech. 43, 151 (2007)

    Article  CAS  Google Scholar 

  20. L. Wadsö, Cement Int. 5, 94 (2005)

    Google Scholar 

  21. I. Wadsö, R.N. Goldberg, Pure Appl. Chem. 73, 1625 (2001)

    Article  Google Scholar 

  22. L. Wadsö, F. Gomez Galindo, I. Sjöholm, P. Rocculi, Thermochim. Acta 422, 89 (2004)

    Article  CAS  Google Scholar 

  23. F. Razem, M.A. Bernards, J. Exp. Bot. 54, 935 (2003)

    Article  CAS  Google Scholar 

  24. D.J. Bradley, P. Kjelbom, C.J. Lamb, Cell 70, 21 (1992)

    Article  CAS  Google Scholar 

  25. P. Wojtaszek, Biochem 322, 681 (1997)

    CAS  Google Scholar 

  26. R. Arora, J.P. Palta, Plant Physiol. 95, 846 (1991)

    Article  CAS  Google Scholar 

  27. R.P. Joshi, Q. Hu, K.H. Schoenbach, IEEE Trans. Plasma Sci. 30, 1536 (2002)

    Article  Google Scholar 

  28. Z. Ji, S. Kennedy, J.H. Booske, S.C. Hagness, IEEE Trans. Plasma Sci. 34, 1416 (2006)

    Article  CAS  Google Scholar 

  29. L.F. Ruis-Heutinck, B. Savenije, F. Postema, A. Van Voorst, E. Lambooij, J. Korf, Poultry Sci. 77, 1422 (1998)

    CAS  Google Scholar 

  30. F. Gómez Galindo, P.T. Vernier, P. Dejmek, A. Vicente, M.A. Gundersen, Bioelectromagnetics, 29, 296 (2008)

    Article  Google Scholar 

  31. V.L. Link, M.G. Hofmann, A.K. Sinha, R. Ehness, M. Strnad, T. Roitsch, Plant Physiol. 128, 271 (2002)

    Article  CAS  Google Scholar 

  32. E. Peiter, F.J.M. Maathuis, L.N. Mills et al., Nature 434, 404 (2005)

    Article  CAS  Google Scholar 

  33. I.I. Pottosin, G. Schönknecht, J. Exp. Bot. 58, 1559 (2007)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Allan G. Rasmusson from the Department of Cell and Organism Biology and Marianne Sommarin from the Department of Plant Biochemistry, Lund University, Sweden for the enlightening discussions. This study was supported by grants from the Portuguese Foundation of Science and Technology and Sparbanksstiftelsen Färs & Frosta, Sweden.

Author information

Authors and Affiliations

  1. IBB-Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Federico Gómez Galindo & António Vicente

  2. Division of Building Materials, Lund University, P.O. Box 118, SE-221 00, Lund, Sweden

    Lars Wadsö

  3. Department of Food Technology, Engineering and Nutrition, Lund University, PO Box 124, SE-221 00, Lund, Sweden

    Petr Dejmek

Authors
  1. Federico Gómez Galindo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lars Wadsö
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. António Vicente
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Petr Dejmek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Federico Gómez Galindo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gómez Galindo, F., Wadsö, L., Vicente, A. et al. Exploring Metabolic Responses of Potato Tissue Induced by Electric Pulses. Food Biophysics 3, 352–360 (2008). https://doi.org/10.1007/s11483-008-9086-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11483-008-9086-3

Keywords

Search

Navigation