Abstract
Cell membrane electroporation/permeabilization may be achieved without affecting cell viability through strict control of the electric pulse parameters. This process is referred to as reversible permeabilization. Even if the cells survive the electric field treatment, they are subjected to stress due to the opening of pores and the struggle of the cells to recover their normal functionality. Very little is known about what actually occurs in the cell and its membranes at the molecular level upon reversible electroporation, and the physiological responses to pulsed electric field (PEF)-induced stress are still largely unknown. This chapter explores the current state of the art on the influence of the complexity of plant tissues on electroporation. Focusing on reversible electroporation, metabolic responses of plant cells and tissues induced by PEF application are also reviewed. One of the first challenges when electroporating plant tissue is their heterogeneous structures where cells vary in shape, size, and cell wall structure. This heterogeneity influences the effect of different electric fields protocols aiming at permeabilizing all cells in the tissue. Once cells are reversibly permeabilized, physiological responses to PEF-induced stress include the production of reactive oxygen species, mobilization of stored energy, activation of stress-related genes, and the production of secondary metabolites. The application of reversible PEF has also been shown to barley seed germination as well as to increase the strength of the cell wall in potatoes and, in consequence, their textural properties. This chapter finishes by revising the effect of reversible PEF on protoplasts (plant cells where the cell walls have been removed) and, in consequence, on the regeneration of new plants. Overall, reports on reversible permeabilization of plant cells and tissues are not common in the literature; however, they have laid the foundation for a fascinating area of research and technological innovation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Asavasanti S, Ristenpart W, Stroeve P, Barrett DM (2011) Permeabilization of plant tissues by monopolar pulsed electric fields: effect of frequency. J Food Sci 76:E98–E111
Balasa A, Toepfl S, Knorr D (2006) Pulsed electric field treatment of grapes. Food factory for the future 3. Gothenburg
Batista Napotnik T, Rebersek M, Vernier PT, Mali B, Miklavčič D (2016) Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro). A systematic review. Bioelectrochemistry 110:1–12
Cogalniceanu G, Radu M, Fologea D, Brezeanu A (1998) Are the electric field effects coupled with the hormonal reception of cells in plant callus culture? Rom Biotechnol Lett 3:Ö 201–206
Dymek K, Dejmek P, Panarese V, Vicente AA, Wadsö L, Finnie C, Gómez Galindo F (2012) Effect of pulsed electric field on the germination of barley seeds. LWT- Food Sci Technol 47:161–166
Dymek K, Dejmek P, Gómez Galindo F (2014) Influence of pulsed electric field protocols on the reversible permeabilization of rucola leaves. Food Bioprocess Technol 7:761–773
Eing CJ, Bonnet S, Pacher M, Puchta H, Frey W (2009) Effects of nanosecond pulsed electric field exposure on Arabidopsis thaliana. IEEE Trans Dielectr Electr Insul 16:1322–1328
Evrendilek GA, Zhang QH, Richter ER (1999) Inactivation of Escherichia coli O157:H7 and Escherichia coli 8739 in apple juice by pulsed electric fields. J Food Prot 62:793–796
Ganeva V, Galutzov B, Teissie J (1995) Electric field mediated loading of macromolecules in intact yeast cells is critically controlled at the wall level. Biochim Biophys Acta 1240: 229–236
Gómez Galindo F, Wadsö L, Vicente A, Dejmek P (2008a) Exploring metabolic responses of potato tissue induced by electric pulses. Food Biophys 3:352–360
Gómez Galindo F, Vernier T, Dejmek P, Vicente A, Gundersen M (2008b) Pulsed electric field reduces the permeability of potato cell wall. Bioelectromagnetics 29:296–301
Gómez Galindo F, Dejmek P, Lundgren K, Rasmusson AG, Vicente A, Moritz T (2009) Metabolomic evaluation of pulsed electric field-induced stress on potato tissue. Planta 230:469–479
Guderjan M, Töpfl S, Angersbach A, Knorr D (2005) Impact of pulsed electric field treatment on the recovery and quality of plant oils. J Food Eng 67:281–287
Helmersson E (2012) Optimisation of vacuum impregnation and pulsed electric field parameters for improving the cryoprotection of potato tubers. Master of Science thesis. University of Lund
Ji Z, Kennedy S, Booske JH, Hagness SC (2006) Experimental studies of persistent poration dynamics of cell membranes induced by electric pulses. IEEE Trans Plasma Sci 34:1416–1424
Joersbo M, Brunstedt J (1990) Stimulation of protein synthesis in electroporated plant protoplasts. J Plant Physiol 136:464–467
Kotnik T, Mir LM, Flisar K, Puc M, Miklavčič D (2001) Cell membrane electropermeabilization by symmetrical bipolar rectangular pulses. Part I. Increased efficiency of permeabilization. Bioelectrochemistry 54:83–90
Lebar Macek A, Miklavčič D (2001) Cell electropermeabilization to small molecules in vitro: control by pulse parameters. Radiol Oncol 35:193–202
Mordhorst AP, Lörz H (1992) Electrostimulated regeneration of plantlets from protoplasts derived from cell suspensions of barley (Hordeum vulgare). Physiol Plant 85:289–294
Pereira RN, Gómez Galindo F, Vicente A, Dejmek P (2009) Effects of pulsed electric field on the viscoelastic properties of potato tissue. Food Biophys 4:229–239
Phoon PY, Gómez Galindo F, Vicente A, Dejmek A (2008) Pulsed electric field in combination with vacuum impregnation with trehalose improves the freezing tolerance of spinach leaves. J Food Eng 88:144–148
Pucihar G, Mir LM, Miklavčič D (2002) The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy. Bioelectrochemistry 57:167–172
Rech EL, Ochat SJ, Chand PK, Power JB, Davey MR (1987) Electro-enhancement of division of plant protoplast-derived cells. Protoplasma 141:169–176
Saw NMMT, Riedel H, Cai Z, Kutuk O, Smetanska I (2012) Stimulation of anthocyanin synthesis in grape (Vitris vinifera) cell cultures by pulsed electric fields and ethephon. Plant Cell Tiss Organ Cult 108:47–54
Vallverdú-Queralt A, Oms-Oliu G, Odriozola-Serrano I, Lamuela-Raventós R, Martín Belloso O, Elez-Martínez P (2013) Metabolite profiling of phenolic and carotenoid contents in tomatoes after moderate-intensity pulsed electric field treatments. Food Chem 136:199–205
Yarmush ML, Golberg A, Sersa G, Kotnik T, Miklavčič D (2014) Electroporation based technologies for medicine: principles, applications and challenges. Annu Rev Biomed Eng 16:295–320
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this entry
Cite this entry
Galindo, F.G. (2017). Responses of Plant Cells and Tissues to Pulsed Electric Field Treatments. In: Miklavčič, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-32886-7_195
Download citation
DOI: https://doi.org/10.1007/978-3-319-32886-7_195
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-32885-0
Online ISBN: 978-3-319-32886-7
eBook Packages: EngineeringReference Module Computer Science and Engineering