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Arabidopsis calcium-dependent protein kinase CPK28 is potentially involved in the response to osmotic stress

  • Article
  • Plant Physiology
  • Published:
Chinese Science Bulletin

Abstract

As calcium sensors, plant calcium-dependent protein kinases (CDPKs) play important roles in plants’ responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the CDPK family in Arabidopsis, in response to osmotic stress. The cpk28 mutant, a loss-of-function mutant, exhibited an NaCl- and mannitol-sensitive phenotype in green cotyledons, while CPK28-overexpressing plants displayed stronger tolerance to NaCl and mannitol stresses than wild-type plants. Reverse transcriptase-polymerase chain reaction and beta-glucuronidase staining assays showed that NaCl and mannitol stresses induced CPK28. CPK28-overexpressing lines accumulated significantly more proline relative to wild-type plants and mutant plants under NaCl and mannitol stresses. Transient expression of CPK28-GFP in mesophyll cell protoplasts, as well as stable transgenic lines expressing CPK28-GFP, showed that CPK28 was localized in the plasma membrane. Expression levels of known stress-responsive genes were not significantly altered in the null mutant and overexpression lines, suggesting that CPK28 possibly mediated the stress response via the regulation of target proteins rather than via regulation at the level of transcription. Meanwhile, CPK28 could autophosphorylate. Taken together, these data demonstrated that CPK28, a potential positive regulator, is involved in the response to osmotic stress in Arabidopsis.

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References

  1. Evans NH, McAinsh MR, Hetherington AM et al (2001) Calcium oscillations in higher plants. Curr Opin Plant Biol 4:415–420

    Article  Google Scholar 

  2. Cheng SH, Willmann MR, Chen HC et al (2002) Calcium signaling through protein kinases: the Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129:469–485

    Article  Google Scholar 

  3. Sanders D, Pelloux J, Brownlee C et al (2002) Calcium at the crossroads of signaling. Plant Cell 14:401–417

    Google Scholar 

  4. Ludwig AA, Romeis T, Jones JD et al (2004) CDPK-mediated signalling pathways: specificity and cross-talk. J Exp Bot 55:181–188

    Article  Google Scholar 

  5. McCormack E, Tsai YC, Braam J et al (2005) Handling calcium signaling: Arabidopsis CaMs and CMLs. Trends Plant Sci 10:383–389

    Article  Google Scholar 

  6. Kolukisaoglu U, Weinl S, Blazevic D et al (2004) Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks. Plant Physiol 134:43–58

    Article  Google Scholar 

  7. Luan S (2009) The CBL-CIPK network in plant calcium signaling. Trends Plant Sci 14:37–42

    Article  Google Scholar 

  8. Harmon AC, Gribskov M, Gubrium E et al (2001) The CDPK superfamily of protein kinases. New Phytol 151:175–183

    Article  Google Scholar 

  9. Harper JF, Breton G, Harmon A (2004) Decoding Ca2+ signals through plant protein kinases. Annu Rev Plant Biol 55:263–288

    Article  Google Scholar 

  10. Reddy VS, Reddy AS (2004) Proteomics of calcium-signaling components in plants. Phytochemistry 65:1745–1776

    Article  Google Scholar 

  11. Zhu SY, Yu XC, Wang XJ et al (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell 19:3019–3036

    Article  Google Scholar 

  12. Choi HI, Park HJ, Park JH et al (2005) Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid- responsive gene expression, and modulates its activity. Plant Physiol 139:1750–1761

    Article  Google Scholar 

  13. Zhao R, Sun HL, Mei C et al (2011) The Arabidopsis Ca2+-dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth. New Phytol 192:61–73

    Article  Google Scholar 

  14. Mehlmer N, Wurzinger B, Stael S et al (2010) The Ca2+-dependent protein kinase CPK3 is required for MAPK-independent salt-stress acclimation in Arabidopsis. Plant J 63:484–498

    Article  Google Scholar 

  15. Munemasa S, Hossain MA, Nakamura Y et al (2011) The Arabidopsis calcium dependent protein kinase, CPK6, functions as a positive regulator of methyl jasmonate signaling in guard cells. Plant Physiol 155:553–561

    Article  Google Scholar 

  16. Zou JJ, Wei FJ, Wang C et al (2010) Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol 154:1232–1243

    Article  Google Scholar 

  17. Geiger D, Scherzer S, Mumm P et al (2010) Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Proc Natl Acad Sci USA 17:8023–8028

    Article  Google Scholar 

  18. Tuteja N (2007) Mechanisms of high salinity tolerance in plants. Methods Enzymol 428:419–438

    Article  Google Scholar 

  19. Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681

    Article  Google Scholar 

  20. Leidi EO, Barragán V, Rubio L et al (2010) The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato. Plant J 61:495–506

    Article  Google Scholar 

  21. Yamaguchi T, Blumwald E (2005) Developing salt-tolerant crop plants: challenges and opportunities. Trends Plant Sci 10:615–620

    Article  Google Scholar 

  22. Horie T, Hauser F, Schroeder JI et al (2009) HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants. Trends Plant Sci 14:660–668

    Article  Google Scholar 

  23. Knight H, Trewavas AJ, Knight MR et al (1997) Calcium signaling in Arabidopsis thaliana responding to drought and salinity. Plant J 12:1067–1078

    Article  Google Scholar 

  24. Shi H, Lee BH, Wu SJ et al (2003) Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nat Biotechnol 21:81–85

    Article  Google Scholar 

  25. Apse MP, Aharon GS, Snedden WA et al (1999) Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285:1256–1258

    Article  Google Scholar 

  26. Qiu QS, Guo Y, Quintero FJ et al (2004) Regulation of vacuolar Na+/H+ exchange in Arabidopsis thaliana by the salt-overly-sensitive (SOS) pathway. J Biol Chem 279:207–215

    Article  Google Scholar 

  27. Dammann C, Ichida A, Hong B et al (2003) Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis. Plant Physiol 132:1840–1848

    Article  Google Scholar 

  28. Jung Y, Park J, Choi Y et al (2010) Expression analysis of proline metabolism-related genes from halophyte Arabis stelleri under osmotic stress conditions. J Integr Plant Biol 52:891–903

    Article  Google Scholar 

  29. Toka I, Plasrchais S, Cabassa C et al (2010) Mutations in the hyperosmotic stress-responsive mitochondrial basic amino acid carrier2 enhance proline accumulation in Arabidopsis. Plant Physiol 152:1851–1862

    Article  Google Scholar 

  30. Saadia M, Jamil A, Akram NA et al (2012) A study of proline metabolism in canola (Brassica napus L.) seedlings under salt stress. Molecules 17:5803–5815

    Article  Google Scholar 

  31. Kreps JA, Wu Y, Chang HS et al (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141

    Article  Google Scholar 

  32. Taji T, Seki M, Satou M et al (2004) Comparative genomics in salt tolerance between Arabidopsis and Arabidopsis-related halophyte salt cress using Arabidopsis microarray. Plant Physiol 135:1697–1709

    Article  Google Scholar 

  33. Matschi S, Werner S, Schulze WX et al (2013) Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development. Plant J 73:883–896

    Article  Google Scholar 

  34. Wernimont AK, Artz JD, Finerty P Jr et al (2010) Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol 17:596–601

    Article  Google Scholar 

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Acknowledgments

This work was supported by the National Genetically Modified Organisms Breeding Major Projects (2011ZX08009-002).

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Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, 475004, China

    Anli Gao, Qingyang Wu, Yu Zhang, Yuchen Miao & Chunpeng Song

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  1. Anli Gao
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  2. Qingyang Wu
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  3. Yu Zhang
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  4. Yuchen Miao
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  5. Chunpeng Song
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Correspondence to Chunpeng Song.

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Gao, A., Wu, Q., Zhang, Y. et al. Arabidopsis calcium-dependent protein kinase CPK28 is potentially involved in the response to osmotic stress. Chin. Sci. Bull. 59, 1113–1122 (2014). https://doi.org/10.1007/s11434-013-0062-z

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  • DOI: https://doi.org/10.1007/s11434-013-0062-z

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