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Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing

  • Review
  • Molecular Genetics
  • Published:
Chinese Science Bulletin

Abstract

The large genome size (∼17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromo-some-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals.

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Authors and Affiliations

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    SuoWei Wu, Xu Zheng, BingHua Liu, Li Yang, MeiFang Song, Peng Zhou, Yang Zhou, FanHua Meng, ShanHong Wang, HongWei Liu, HuQu Zhai & JianPing Yang

  2. Graduate School, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yang Xiao & Xu Zheng

  3. College of Bio-information, Chongqing University of Posts and Telecommunication, Chongqing, 400065, China

    YingFan Cai & JianPing Yang

  4. Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, CZ-77200, Czech Republic

    Jaroslav Doležel

  5. Institute of Vegetable Research, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    MeiFang Song

Authors
  1. SuoWei Wu
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  2. Yang Xiao
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  3. Xu Zheng
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  4. YingFan Cai
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  5. Jaroslav Doležel
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  6. BingHua Liu
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  7. Li Yang
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  9. Peng Zhou
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  10. Yang Zhou
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  11. FanHua Meng
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  12. ShanHong Wang
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  13. HongWei Liu
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  14. HuQu Zhai
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  15. JianPing Yang
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Corresponding authors

Correspondence to HuQu Zhai or JianPing Yang.

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These authors contributed equally to this work

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Cite this article

Wu, S., Xiao, Y., Zheng, X. et al. Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing. Chin. Sci. Bull. 55, 1463–1468 (2010). https://doi.org/10.1007/s11434-010-3106-7

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  • DOI: https://doi.org/10.1007/s11434-010-3106-7

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