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Suppressor of RNA silencing encoded by Rice gall dwarf virus genome segment 11

  • Articles
  • Virology
  • Published:
Chinese Science Bulletin

Abstract

Rice gall dwarf virus (RGDV) is an important rice pathogen in China and Southeast Asia. However, little is known about the molecular mechanisms of RGDV interactions with plant cells. Here, we have identified an RGDV protein, Pns11, which acts as a suppressor of RNA silencing in coinfiltration assays with the reporter, green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c carrying GFP. Pns11 suppressed local and systemic silencing induced by sense RNA. The spread of mobile RNA silencing signals was blocked or inactivated by Pns11. Expression of Pns11 also enhanced Potato virus X pathogenicity in N. benthamiana. This suppressor could reduce, but not eliminate, siRNA in the local and systemic RNA silencing suppression assays, suggesting that Pns11 functions by interfering with initial stages of RNA silencing.

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Authors and Affiliations

  1. Laboratory of Plant Virology, South China Agricultural University, Guangzhou, 510642, China

    Liu FuXiu, Zhao Qin, Ruan XiaoLei, He YunWei & Li HuaPing

  2. Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou, 570206, China

    Liu FuXiu

Authors
  1. Liu FuXiu
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  2. Zhao Qin
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  3. Ruan XiaoLei
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  4. He YunWei
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  5. Li HuaPing
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Corresponding author

Correspondence to Li HuaPing.

Additional information

Supported in part by the National Natural Science Foundation of China (Grant Nos. 30370929 and 30671358)

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Liu, F., Zhao, Q., Ruan, X. et al. Suppressor of RNA silencing encoded by Rice gall dwarf virus genome segment 11. Chin. Sci. Bull. 53, 362–369 (2008). https://doi.org/10.1007/s11434-008-0095-x

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  • DOI: https://doi.org/10.1007/s11434-008-0095-x

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