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SecReT6 update: a comprehensive resource of bacterial Type VI Secretion Systems

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Abstract

Type VI Secretion System (T6SS) plays significant roles in microbial activities via injecting effectors into adjacent cells or environments. T6SS increasingly gained attention due to its important influence on pathogenesis, microbial competition, etc. T6SS-associated research is explosively expanding on numerous grounds that call for an efficient resource. The SecReT6 version 3 provides comprehensive information on T6SS and the interactions between T6SS and T6SS-related proteins such as T6SS regulators and T6SS effectors. To assist T6SS researches like microbial competition and regulatory mechanisms, SecReT6 v3 developed online tools for detection and analysis of T6SS and T6SS-related proteins and estimation of T6SS-dependent killing risk. We have identified a novel T6SS regulator and T6SS-dependent killing capacity in Acinetobacter baumannii clinical isolates with the aid of SecReT6 v3. 17,212 T6SSs and plentiful T6SS-related proteins in 26,573 bacterial complete genomes were also detected, analyzed and incorporated into the database. The database is freely available at https://bioinfo-mml.sjtu.edu.cn/SecReT6/.

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Acknowledgements

This work was supported by the Science and Technology Commission of Shanghai Municipality (19430750600, 19JC1413000), the National Natural Science Foundation of China (31670074), the Medical Excellence Award Funded by the Creative Research Development Grant from the First Affiliated Hospital of Guangxi Medical University (XK2019025), and the Science Fund of the Republic of Serbia (7750294, q-bioBDS). We are grateful to Quanjiang Ji at ShanghaiTech University for giving the gene-editing system of pCasAb for A. baumannii.

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  1. Contributed equally to this work

Authors and Affiliations

  1. State Key Laboratory of Microbial Metabolism, Shanghai-Islamabad-Belgrade Joint Innovation Center on Antibacterial Resistances, Joint International Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Jianfeng Zhang, Jiahao Guan, Meng Wang, Cui Tai, Zixin Deng & Hong-Yu Ou

  2. Intensive Care Unit, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China

    Jianfeng Zhang & Zhaoyan Chen

  3. Department of Laboratory Medicine, Jinshan Hospital, Shanghai Medical College, Fudan University, Shanghai, 201500, China

    Gang Li

  4. Quantitative Biology Group, Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Belgrade, 11000, Serbia

    Marko Djordjevic

  5. State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China

    Hui Wang

  6. Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Hong-Yu Ou

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  1. Jianfeng Zhang
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Correspondence to Zhaoyan Chen or Hong-Yu Ou.

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Zhang, J., Guan, J., Wang, M. et al. SecReT6 update: a comprehensive resource of bacterial Type VI Secretion Systems. Sci. China Life Sci. 66, 626–634 (2023). https://doi.org/10.1007/s11427-022-2172-x

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  • DOI: https://doi.org/10.1007/s11427-022-2172-x

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