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An improved hybrid bream derived from a hybrid lineage of Megalobrama amblycephala (♀)×Culter alburnus (♂)

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Abstract

Distant hybridization is an important technique in fish genetic breeding. In this study, based on the establishment of an allodiploid fish lineage (BT, 2n=48, F1–F6) derived from distant hybridization between female Megalobrama amblycephala (BSB, 2n=48) and male Culter alburnus (TC, 2n=48), and the backcross progeny (BTB, 2n=48) derived by backcrossing female F1 of BT to male BSB, an improved hybrid bream (BTBB, 2n=48) was obtained by backcrossing BTB (♀) to BSB (♂). Moreover, the morphological and genetic characteristics of BTBB individuals were investigated; BTBB was similar to BSB in appearance but had a higher body height than BSB. The study results regarding chromosome numbers and DNA content indicated that BTBB is a diploid hybrid fish. The 5S rDNA and Hox gene of BTBB were inherited from the original parents. Gonadal development in BTBB was normal. On the other hand, BTBB had a faster growth rate, higher muscle protein level, and lower muscle carbohydrate level than BSB. Hence, bisexual fertile BTBB is promoted and can be applied as a high-quality fish, and it can also be used as a new fish germplasm resource to develop high-quality fish further. Thus, this study is of great significance for fish genetic breeding.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31730098, 31802287, 31430088, 31872315, U19A2040), the Earmarked Fund for China Agriculture Research System (CARS-45), the Hunan Provincial Natural Science and Technology Major Project (2017NK1031), the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), the National Key Research and Development Program of China (2018YFD0901202), and the High-level Talent Agglomeration Program of Hunan, China (2019RS1044).

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Authors and Affiliations

  1. State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China

    Dingbin Gong, Min Tao, Lihui Xu, Fangzhou Hu, Zehong Wei, Shi Wang, Yude Wang, Qingfeng Liu, Chang Wu, Kaikun Luo, Chenchen Tang, Rong Zhou, Chun Zhang, Yuequn Wang & Shaojun Liu

  2. College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Dingbin Gong, Min Tao, Lihui Xu, Fangzhou Hu, Zehong Wei, Shi Wang, Yude Wang, Qingfeng Liu, Chang Wu, Kaikun Luo, Chenchen Tang, Rong Zhou, Chun Zhang, Yuequn Wang & Shaojun Liu

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  1. Dingbin Gong
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  2. Min Tao
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  3. Lihui Xu
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  4. Fangzhou Hu
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Correspondence to Yuequn Wang or Shaojun Liu.

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Gong, D., Tao, M., Xu, L. et al. An improved hybrid bream derived from a hybrid lineage of Megalobrama amblycephala (♀)×Culter alburnus (♂). Sci. China Life Sci. 65, 1213–1221 (2022). https://doi.org/10.1007/s11427-021-2005-5

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