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Alterations in intestinal microbiota of colorectal cancer patients receiving radical surgery combined with adjuvant CapeOx therapy

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Abstract

An intricate relationship exists and interactions occur between gut microbiota and colorectal cancer (CRC). Radical surgery combined with adjuvant chemotherapy (AC) serves as the mainstream therapeutic scheme for most CRC patients. The current research was conducted to assess the effect of surgery or chemotherapy on gut microbiota. Forty-three CRC patients who received radical surgery and AC were enrolled. Fecal samples were collected preoperatively, postoperatively, and after the first to fifth cycles of postoperative chemotherapy. The microbial community of each sample was analyzed using high throughput 16S rRNA amplicon sequencing. Compared with preoperative samples, fecal samples collected postoperatively exhibited a significant decrease of obligate anaerobes, tumor-related bacteria, and butyric acid-producing bacteria. However, a significant increase of some conditional pathogens was observed. In addition, the AC regimen (CapeOx) was found to alter intestinal microbiota dramatically. In particular, several changes were observed after chemotherapy including an increase of pathogenic bacteria, the “rebound effect” of chemotherapy-adapted bacteria, the shift of lactate-utilizing microbiota from Veillonella to Butyricimonas and Butyricicoccus, as well as the decrease of probiotics. Both radical surgery and CapeOx chemotherapy exert a non-negligible effect on the gut microbiota of CRC patients. Microbiota-based intervention may be beneficial for patients during postoperative clinical management.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81230057, 81372615, 81472262 and 81200264), the Emerging Cutting-Edge Technology Joint Research Projects of Shanghai (SHDC12012106) and Tongji University Subject Pilot Program (162385), Lijieshou Intestinal Barrier Foundation (LJS-201701), Specialized Research Fund for the Combine Traditional Chinese and Western Medicine in General Hospital of Shanghai (ZHYY-ZXYJHZX-1-201704).

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  1. Department of General Surgery, Shanghai Tenth People’s Hospital Affiliated to Tongji University, Shanghai, 200072, China

    Cheng Kong, Renyuan Gao, Linsheng Huang, Jide He, Hao Li & Huanlong Qin

  2. Research Institute of Intestinal Diseases, Tongji University School of Medicine, Shanghai, 200072, China

    Cheng Kong, Renyuan Gao, Linsheng Huang, Jide He, Hao Li & Huanlong Qin

  3. Department of Oncology, Affiliated Hospital of Yangzhou University, Yangzhou, 225000, China

    Xuebing Yan

  4. Department of Traditional Chinese Medicine, Shanghai Ninth People’s Hospital, Shanghai, 200011, China

    Jie You

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  1. Cheng Kong
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Kong, C., Gao, R., Yan, X. et al. Alterations in intestinal microbiota of colorectal cancer patients receiving radical surgery combined with adjuvant CapeOx therapy. Sci. China Life Sci. 62, 1178–1193 (2019). https://doi.org/10.1007/s11427-018-9456-x

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