Abstract
β-galactosidases (EC 3.2.1.23) constitute a widespread family of glycosyl hydrolases in plants and are thought to be involved in metabolism of cell wall polysaccharides. A cDNA of the cotton (Gossypium hirsutum) β-galactosidase gene, designated GhGal1, has previously been identified and its transcripts are highly abundant at the elongation stage of the cotton fiber. To examine the temporal and spatial control of GhGal1 expression, a transcriptional fusion of the GhGal1 promoter region (1770 bp) with the β-glucuronidase (GUS) reporter gene was introduced into tobacco plants by the Agrobacterium infection method. The resulting transgenic plants showed higher GUS activity of fruit in the transgenic plants than that in the negative and positive controls. Histochemical localization of GUS activity demonstrated that the expression of the GUS gene could be found in the meristem zones of roots, cotyledons, vascular tissues, fruit and trichomes in transgenic tobacco plants. Additionally, sequence analysis of the regulatory region also revealed several conserved motifs among which some were shared with previously reported fruit/seed-specific elements and the others were related with trichome expression. These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development.
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Smith, D. L., Gross, K. C., A family of at least seven β-galactosidase genes is expressed during tomato fruit development, Plant Physiol., 2000, 123: 1173–1183., 10.1104/pp.123.3.1173, 1:CAS:528:DC%2BD3cXlt1SltLw%3D, 10889266
Smith, D. L., Abbott, J. A., Gross, K. C., Down-regulation of tomato β-galactosidase 4 results in decreased fruit softening, Plant Physiol., 2002, 129: 1755–1762., 1:CAS:528:DC%2BD38Xmtl2gt7g%3D, 12177488
Buckeridge, M. S., Reid, J. S., Purification and properties of a novel β-Galactosidase or exo-β-D-Galactosidase from the cotyledons of germinated Lupinus angustifolius L. seeds, Planta, 1994, 192: 502–511., 10.1007/BF00203588, 1:CAS:528:DyaK2cXis1Cht7k%3D, 7764618
Rogers, H. J., Maund, S. L., Johnson, L. H., A β-galactosidase-like gene is expressed during tobacco pollen development, J. Exp. Bot., 2001, 52: 67–75., 1:CAS:528:DC%2BD3MXhvVOitrg%3D, 11181714
Brandt, A. S., Woodson, W. R., Purification and characterization of a β-galactosidase from sensing carnation petals, Plant Physiol., 1997, 114(Suppl): 804.
Zhang, H. M., Liu, J. Y., Molecular cloning and characterization of a β-galactosidase gene expressed preferentially in cotton fibers, J. Integrat. Plant Biol., 2005, 47: 223–232., 1:CAS:528:DC%2BD28Xpt1ylsA%3D%3D
Maley, F., Trimble, R. B., Tarentino, A. L. et al., Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases, Anal. Biochem., 1989, 80: 195–204.
Raghothama, K. G., Lawton, K. A., Goldsbrough, P. B. et al., Characterization of an ethylene-regulated flower senescence-related gene from carnation, Plant Mol. Biol., 1991, 17: 61–71., 10.1007/BF00036806, 1:CAS:528:DyaK38XhsVGit7w%3D, 1868223
Ross, G. S., Redgwell, R. J., MacRae, E. A., Kiwifruit β-galactosidase: Isolation and activity against specific fruit cell-wall polysaccharides, Planta, 1993, 189: 499–506., 10.1007/BF00198212, 1:CAS:528:DyaK3sXisFShu7s%3D
Hall, B. G., Determining the evolutionary potential of a gene. Mol. Biol. Evol., 1998, 15: 1055–1061., 1:CAS:528:DyaK1cXlt1ejsbk%3D, 9718732
Li, S. C., Han, J. W., Chen, K. C. et al., Purification and characterization of isoforms of β-galactosidases in mung bean seedlings, Phytochemistry, 2001, 57: 349–359., 1:CAS:528:DC%2BD3MXjtFCmsrw%3D, 11393513
Nunan, K. J., Davies, C., Robinson, S. P. et al., Expression patterns of cell wall-modifying enzymes during grape berry development, Planta, 2001, 214: 257–264., 1:CAS:528:DC%2BD3MXovFKns7c%3D, 11800390
Trainotti, L., Spinello, R., Piovan, A. et al., β-galactosidases with a lectin-like domain are expressed in strawberry, J. Exp. Bot., 2001, 52: 1635–1645., 10.1093/jexbot/52.361.1635, 1:CAS:528:DC%2BD3MXlvVClsro%3D, 11479328
Liu, J. Y., Zhao, G. R., Li, J., Molecular engineering on quality improvement of cotton fiber, Acta Bot. Sin. (in Chinese), 2000, 42: 991–995., 1:CAS:528:DC%2BD3cXovFOnsL4%3D
Wilkins, T. A., Jernstedt, J. A., Molecular genetics of developing cotton fibers, in Cotton Fibers (ed. Basra, A. S.), New York: Hawthorne Press, 1999, 231–267.
Paterson, A. H., Brubaker, C. L., Wendel, J. F., A rapid method for extraction cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis, Plant Mol. Biol. Rep., 1993, 11: 122–127., 1:CAS:528:DyaK3sXlvFKks7o%3D
Walkerpeach, C. R., Velten, J., Agrobacterium-mediated gene transfer to plant cells: Co-integrate and binary vector systems, in Plant Molecular Biology Manual (eds. Gelvin, S. B., Schilperoort, R. A.), Dordrecht: Kluwer, 1994, B1: 1–19.
Horsch, R. B., Fry, J. E., Hoffman, N. L. et al., A simple and general method of transferring genes into plants, Science, 1985, 227: 1229–1231., 1:CAS:528:DyaL2MXhtFSjsLc%3D
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 1962, 15: 473–497., 1:CAS:528:DyaF3sXksFKm
Jefferson, R. A., Kavanagh, T. A., Bevan, M. W., GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants, EMBO J., 1987, 6: 3901–3907., 1:CAS:528:DyaL1cXovV2itQ%3D%3D, 3327686
Bradford, M. M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72: 248–254., 10.1016/0003-2697(76)90527-3, 1:CAS:528:DyaE28XksVehtrY%3D, 942051
Higo, K., Ugawa, Y., Iwamoto, M. et al., Plant cis-acting regulatory DNA elements (PLACE) database, Nucleic Acids Res., 1999, 27: 297–300., 10.1093/nar/27.1.297, 1:CAS:528:DyaK1MXpsVKgug%3D%3D, 9847208
Lescot, M., Déhais, P., Thijs, G. et al., PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences, Nucleic Acids Res., 2002, 30: 325–327., 10.1093/nar/30.1.325, 1:CAS:528:DC%2BD38Xht12rs7k%3D, 11752327
Ellerstrom, M., Stalberg, K., Ezcurra, I. et al., Functional dissection of a napin gene promoter: Identification of promoter elements required for embryo and endosperm-specific transcription, Plant Mol. Biol., 1996, 32: 1019–1027., 1:STN:280:ByiC2MnhsVQ%3D, 9002600
Ezcurra, I., Ellerstrom, M., Wycliffe, P. et al., Interaction between composite elements in the napA promoter: Both the B-box ABA-responsive complex and the RY/G complex are necessary for seed-specific expression, Plant Mol. Biol., 1999, 40: 699–709., 10.1023/A:1006206124512, 1:CAS:528:DyaK1MXlvV2rt78%3D, 10480393
Wu, C., Washida, H., Onodera, Y. et al., Quantitative nature of the Prolamin-box, ACGT and AACA motifs in a rice glutelin gene promoter: Minimal cis-element requirements for endosperm-specific gene expression, Plant J., 2000, 23: 415–421., 10.1046/j.1365-313x.2000.00797.x, 1:CAS:528:DC%2BD3cXmsFSlur0%3D, 10929134
Yamagata, H., Yonesu, K., Hirata, A. et al., TGTCACA motif is a novel cis-regulatory enhancer element involved in fruit-specific expression of the cucumisin gene, J. Biol. Chem., 2002, 277: 11582–11590., 10.1074/jbc.M109946200, 1:CAS:528:DC%2BD38Xis1Kgt7o%3D, 11782472
Yamauchi, D., A TGACGT Motif in the 5′-upstream region of alpha-amylase gene from Vigna mungo is a cis-element for expression in cotyledons of germinated seeds, Plant Cell Physiol., 2001, 42: 635–641., 10.1093/pcp/pce079, 1:CAS:528:DC%2BD3MXks1CrurY%3D, 11427683
Kamiya, N., Nagasaki, H., Morikami, A. et al., Isolation and characterization of a rice WUSCHEL-tyope homoebox gene that is specifically expressed in the central cells of a quiescent center in the root apical meristem, Plant J., 2003, 35: 429–441., 10.1046/j.1365-313X.2003.01816.x, 1:CAS:528:DC%2BD3sXnsFyitLw%3D, 12904206
Stalberg, K., Ellerstom, M., Ezcurra, I. et al., Disruption of an overlapping E-box/ABRE motif abolished high transcription of the napA storage-protein promoter in transgenic Brassica napus seeds, Planta, 1996, 199: 515–519., 1:STN:280:BymH3Mrjt1A%3D, 8818291
Montgomery, J., Goldman, S., Deikman, J. et al., Identification of an ethylene-responsive region in the promoter of a fruit ripening gene, Proc. Natl. Acad. Sci. USA, 1993, 90: 5939–5943., 1:CAS:528:DyaK3sXltFSnu7Y%3D, 8327464
Lu, C. A., Ho, T. D., Ho, S. L. et al., Three novel MYB proteins with one DNA binding repeat mediate sugar and hormone regulation of α-Amylase gene expression, Plant Cell, 2002, 14: 1963–1980., 10.1105/tpc.001735, 1:CAS:528:DC%2BD38XmslCgsrg%3D, 12172034
Daniell, H., Streatfield, S. J., Wycoff, K., Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants, Trends Plant Sci., 2001, 6: 219–226., 1:CAS:528:DC%2BD3MXlsFKmtbw%3D, 11335175
Hsu, C., Roy, G. C., Jenkins, J. N. et al., Analysis of promoter activity of cotton lipid transfer protein gene LTP6 in transgenic tobacco plants, Plant Sci., 1999, 143: 63–70., 10.1016/S0168-9452(99)00026-6, 1:CAS:528:DyaK1MXjtFWktrg%3D
Liu, H., Creech, R. G., Jenkins, J. N. et al., Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3, Biochim. Biophys. Acta, 2000, 1487: 106–111., 1:CAS:528:DC%2BD3cXmtVGhsrc%3D, 11004611
Kim, H. J., Triplett, B. A., Cotton fiber growth in planta and in vitro: Models for plant cell elongation and cell wall biogenesis, Plant Physiol., 2001, 127: 1361–1366., 1:CAS:528:DC%2BD38XjtVWitQ%3D%3D, 11743074
Wang, S., Wang, J. W., Yu, N. et al., Control of plant trichomes development by a cotton fiber MYB genee, Plant Cell, 2004, 16: 2323–2334., 1:CAS:528:DC%2BD2cXnvVartLk%3D, 15316114
Wang, E., Gan, S., Wagner, G. J., Isolation and characterization of the CYP71D16 trichome-specific promoter from Nicotiana tabacum L., J. Exp. Bot., 2002, 53: 1891–1897., 1:CAS:528:DC%2BD38Xms1Kmsbk%3D, 12177128
Rerie, W. G., Feldmann, K. A., Marks, M. D., The GLABRA2 gene encodes a homeodomain protein required for normal trichome development in Arabidopsis, Genes Dev., 1994, 8: 1388–1399., 1:CAS:528:DyaK2cXlsFSitbw%3D, 7926739
Abe, M., Takahashi, T., Komeda, Y., Identification of a cis-7regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein, Plant J., 2001, 26: 487–494., 10.1046/j.1365-313x.2001.01047.x, 1:CAS:528:DC%2BD3MXlvVKhsL4%3D, 11439135
Meier, H., Reid, J. S. G., Reserve polysaccharides other than starch in higher plants, in Encyclopedia of Plant Physiology (eds. Loewus, F. A., Tanner, W.,), Berlin: Springer-Verlag Press, 1982, 418–471.
Edwards, M., Dea, I. C. M., Bulpin, P. V. et al., Xyloglucan (amyloid) mobilization in the cotyledons of Tropaeolum majus L. seeds following germination, Planta, 1985, 163: 133–140., 10.1007/BF00395907, 1:CAS:528:DyaL2MXhtV2qtbY%3D
Edwards, M., Dea, I. C. M., Bulpin, P. V. et al., Purification and properties of a novel, xyloglucan-specific endo-(1—4)-beta-D-glucanase from germinated nasturtium seeds (Tropaeolum majus L.), J. Biol. Chem. 1986, 261: 9489–9494., 1:CAS:528:DyaL28XkslCktr8%3D, 3722207
Edwards, M., Bowman, Y. J. L., Dea, I. C. M. et al., A β-D-galactosidase from Nasturtium (Tropueolum mujus L.) cotyledons—purification, properties, and demonstration that xyloglucan is the natural substrate, J. Biol. Chem., 1988, 263: 4333–4337., 1:CAS:528:DyaL1cXhs1ersr8%3D, 3126187
Enéas-filho, J., Barbosa, G. K. C., Sudério, F. B. et al., Isolation and partial purification of β-galactosidases from cotyledons of two cowpea cultivars, R. Bras. Fisiol. Veg., 2001, 13: 251–261.
De Alcântara, P. H. N., Dietrich, S. M. C., Buckeridge, M. S., Xyloglucan mobilization and purification of a (XLLGXLXG) specific β-galactosidase from cotyledons of Copaifera langadorffii, Plant Physiol. Biochem., 1999, 37: 653–663.
Buckeridge, M. S., Dietrich, S. M. C., Galactomannans from Brazilian legume seeds, Revta Brasil Botan., 1990, 13: 109–112.
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Wu, A., Liu, J. Isolation of the promoter of a cotton β-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants. SCI CHINA SER C 49, 105–114 (2006). https://doi.org/10.1007/s11427-006-0105-7
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DOI: https://doi.org/10.1007/s11427-006-0105-7