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Analysis of human urine metabolites using SPE and NMR spectroscopy

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Abstract

Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including 1H J-Resolved, 1H-1H COSY, 1H-1H TOCSY, 1H-13C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of 1H and 13C resonances including many complex coupled spin systems were obtained.

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Authors and Affiliations

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Yang WeiJin, Wang YaWei & Zhou QunFang

  2. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China

    Tang HuiRu

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  1. Yang WeiJin
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  2. Wang YaWei
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  3. Zhou QunFang
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  4. Tang HuiRu
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Correspondence to Yang WeiJin or Tang HuiRu.

Additional information

Supported by the Chinese Academy of Sciences (Grant No: KZCX2-YW-420-21), the National High Technology Research and Development Program of China (Grant No: 2006AA06Z424), and the National Natural Science Foundation of China (Grant Nos: 40590392 and 20575074)

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Yang, W., Wang, Y., Zhou, Q. et al. Analysis of human urine metabolites using SPE and NMR spectroscopy. Sci. China Ser. B-Chem. 51, 218–225 (2008). https://doi.org/10.1007/s11426-008-0031-6

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  • DOI: https://doi.org/10.1007/s11426-008-0031-6

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