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Metabolomics Analysis of Complex Biological Specimens Using Nuclear Magnetic Resonance Spectroscopy

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Metabolomics

Part of the book series: Neuromethods ((NM,volume 159))

Abstract

Metabolomics is an emerging technology that promises the extensive analyses of small molecules in the biological specimen. Metabolomics is recognized as an imperative tool in several distinct areas such as infectious disease research, mapping cellular metabolic processes, investigating tumor metabolism, and environmental and nutritional research such as in phytochemistry, foodomics, and toxicology. Tools such as mass spectroscopy and nuclear magnetic resonance (NMR) spectroscopy are widely utilized for studying metabolomics. However, NMR has been proven to be more robust owing to its high-throughput and sensitive assessment of metabolic profiles. NMR-based metabolomics involve a sequence of steps starting from sample preparation to data analysis in order to unravel information with respect to both quality and quantity of metabolites. The current chapter elucidates the basic protocol used to perform both the qualitative and quantitative analysis of metabolites in complex biological samples using NMR spectroscopy.

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Acknowledgments

The authors thank Dr. Dinesh Kumar, CBMR-Lucknow, for the technical inputs. This work is supported by the following research grants to KMP: CRG/2018/001329 grant from DST-SERB, GKC-01/2016-17/212/NMCG-Research from NMCG-MoWR, and BEST-18-KMP/IITR/109 from BEST Pvt. Ltd.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India

    Khushboo Gulati, Sharanya Sarkar & Krishna Mohan Poluri

  2. Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India

    Krishna Mohan Poluri

Authors
  1. Khushboo Gulati
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  2. Sharanya Sarkar
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  3. Krishna Mohan Poluri
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Corresponding author

Correspondence to Krishna Mohan Poluri .

Editor information

Editors and Affiliations

  1. Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA

    Paul L. Wood

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Gulati, K., Sarkar, S., Poluri, K.M. (2021). Metabolomics Analysis of Complex Biological Specimens Using Nuclear Magnetic Resonance Spectroscopy. In: Wood, P.L. (eds) Metabolomics . Neuromethods, vol 159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0864-7_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0864-7_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0863-0

  • Online ISBN: 978-1-0716-0864-7

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