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Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice

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Abstract

To evaluate the beneficial effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) flavonoid extract (CBFE) on chronic alcohol-induced liver oxidative injury in mice, experimental mice were pretreated with different doses of CBFE (50–200 mg/kg) for 4 weeks by gavage feeding. Biochemical markers and enzymatic antioxidants from serum, liver tissue, mitochondria and microsomes were examined. Our results showed that the activities of TC, TG, L-DLC in serum, the activity of CYP2E1 in microsomes, and the levels of MDA in liver tissue and mitochondria, decreased significantly (P < 0.05) in the CBFE-treated group compared with the alcohol group. On the contrary, the activities of ALT, AST, and H-DLC in serum, enzymatic antioxidants GSH-Px, SOD and GST in liver tissue and mitochondria, and HO-1 in microsomes rose markedly (P < 0.05). Histopathological examination revealed that CBFE (200 mg/kg) pretreatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. It was suggested that the hepatoprotective effects exhibited by CBFE on alcohol-induced liver oxidative injury may be due to its potent antioxidant properties.

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Abbreviations

ADH:

Alcohol dehydrogenase

ALD:

Alcoholic liver diseases

ALT:

Alanine aminotransferase

AST:

Aspartate transaminase

CBFE:

Chinese bayberry flavonoid extract

CYP2E1:

Cytochrome P450 2E1

GSH-Px:

Glutathione peroxidase

GST:

Glutathione S-transferase

H-DLC:

High density lipoprotein cholesterol

HO-1:

Heme oxygenase-1

L-DLC:

Low density lipoprotein cholesterol

LPO:

Lipid peroxidation

MDA:

Malondialdehyde

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TC:

Total cholesterol

TG:

Triglyceride

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Acknowledgments

The project was supported by Zhejiang Provincial Natural Science Foundation of China (Grant No. Y3110213) and the planning subject of ‘the twelfth five-year-plan’ in National Science and Technology for the Rural Development in China (Grant No. 2011BAD23B02).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People’s Republic of China

    Hesheng Liu, Xiangyang Qi & Shaoqian Cao

  2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Peipei Li

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  1. Hesheng Liu
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Correspondence to Xiangyang Qi.

Electronic supplementary material

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Supplemental Fig. S1 HPLC chromatogram (A) and total ion spectrum (B) for CBFE. (TIFF 97 kb)

11418_2014_829_MOESM2_ESM.tif

Supplemental Fig. S2 Structures of flavonoids identified from CBFE: (1) myricetin; (2) kaempferol-3-O-glucoside/galactoside; (3) myricetin-3-O-glucoside/galactoside; (4) myricetin-3-O-rhamnoside; (5) quercetin-3-O-rhamnoside. (TIFF 12 kb)

Supplementary Table S1 (DOC 34 kb)

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Liu, H., Qi, X., Cao, S. et al. Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice. J Nat Med 68, 521–529 (2014). https://doi.org/10.1007/s11418-014-0829-9

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  • DOI: https://doi.org/10.1007/s11418-014-0829-9

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