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Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

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Abstract

Alcohol-induced oxidative stress plays a crucial role in the pathological development of alcoholic liver disease. The aim of this study was to investigate the effects of triterpenoid from Schisandra chinensis on oxidative stress in alcohol-induced liver injury in rats. We found that the administration of triterpenoid attenuated alcohol-induced oxidative stress in multiple organs including liver. Moreover, the impaired liver function and histological changes resulted from alcohol consumption was improved by triterpenoid treatment. Finally, we found that pretreatment with triterpenoid from Schisandra chinensis to alcohol-fed rats increased the expression level of haem oxygenase-1 (HO-1) while inhibited the induction of cytochrome P-450 2E1 (CYP2E1) in liver microsomes. Further assays revealed that the microsomal activity of HO-1 was accordingly induced whereas CYP2E1 was suppressed in rats received triterpenoid intervention. Our findings suggest that triterpenoid from Schisandra chinensis may protect against alcohol-induced liver injury through ameliorating oxidative stress in rats.

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Acknowledgments

This study was supported by Grants from the National Natural Science Foundation of China (No. 31201325), the Doctoral Startup Foundation of Science and Technology Department of Liaoning Province (No. 20121139), the Liaoning Excellent Talents in University (2014108) and the "Tian Zhu Shan" Excellent Talents of Shenyang Agricultural University (2014).

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Authors and Affiliations

  1. College of Food Science, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, People’s Republic of China

    Bin Li, Lijie Zhu, Ting Wu, Jiachen Zhang, Xinyao Jiao, Xiuying Liu, Yanqun Wang & Xianjun Meng

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  1. Bin Li
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  3. Ting Wu
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Correspondence to Xianjun Meng.

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Bin Li and Lijie Zhu have contributed equally to this study.

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12013_2014_266_MOESM1_ESM.tif

Effects of triterpene compounds isolated from Schisandra chinensis on lipid peroxidation and antioxidant enzyme activity in serum of rats exposed to alcohol for different days. Serum samples were obtained at 30, 60, and 90 days after rats were administrated with different doses of alcohol. The levels of reduced GSH, MDA and the activity of SOD in serum were measured by commercial kits, respectively. Rats were randomly divided into the following groups: 1, normal control; 2, alcohol (10 %, 0.8 g/kg b.w.); 3, alcohol (20 %, 1.6 g/kg b.w.); 4, alcohol (30 %, 2.4 g/kg b.w.); 5, alcohol (30 %, 2.4 g/kg b.w.) + triterpene compounds (48 g/kg b.w.); 6, alcohol (30 %, 2.4 g/kg b.w.) + triterpene compounds (96 g/kg b.w.); 7, triterpene compounds (96 g/kg b.w.) (TIFF 1565 kb)

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Li, B., Zhu, L., Wu, T. et al. Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats. Cell Biochem Biophys 71, 803–811 (2015). https://doi.org/10.1007/s12013-014-0266-0

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