Abstract
Calluses were induced from leaves of Schisandra chinensis Baillon (Schisandraceae). Murashige–Skoog (MS) and Woody Plant (WP) media were used for the induction, in full and half strength (1/2 MS or 1/2 WP) salt formulations. Test media were solidified with 0.25% gelrite and supplemented with 2% sucrose and various concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), 3-indolebutyric acid (IBA), and 6-benzylaminopurine (BAP). Optimal conditions for callus induction and growth were found to be 1/2 MS medium containing 0.02 mg/l Kin and 0.2 mg/l 2,4-D. Chloroform extracts of all induced calluses contained gomisin A and F as major components. Gomisin A and F contents of calluses that were cultured under the optimal conditions mentioned above were highest compared to the calluses incubated with other combinations of plant hormones and media. Subculture, by repeated transfer of cultured calluses to fresh medium, caused no decrease in the production of gomisin A and F. Optimal conditions for lignan production were found to be 1/2 MS medium supplemented with 0.05 mg/1 Kin and 0.2 mg/l 2,4-D. Under these conditions, gomisin A and gomisin F contents were 0.05 and 0.04% of callus dry weight, respectively.
References
Hancke JL, Burgos RA, Ahumad F (1999) Schisandra chinensis (Turcz.) Baill. Fitoterapia 70:451–471
Yasukawa K, Ikeya Y, Mitsuhashi H, Iwasaki M, Aburada M, Nakagawa S, Takeuchi M, Takido M (1992) Gomisin A inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin. Oncology 49:68–71
Chen DF, Zhang SX, Xie L, Xie JX, Chen K, Kashiwada Y, Zhou BN, Wang P, Cosentino LM, Lee KH (1997) Anti-AIDS agents—XXVI. Structure-activity correlations of gomisin-G-related anti-HIV lignans from Kadsura interior and of related synthetic analogues. Bioorg Med Chem 5:1715–1723
Zhu M, Lin KF, Yeung RY, Li RC (1999) Evaluation of the protective effects of Schisandra chinensis on phase I drug metabolism using a CCl4 intoxication model. J Ethnopharmacol 67:61–68
Kim SR, Lee MK, Koo KA, Kim SH, Sung SH, Lee NG, Markelonis GJ, Oh TH, Yang JH, Kim YC (2004) Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity. J Neurosci Res 76:397–405
Li L, Lu Q, Shen Y, Hu X (2006) Schisandrin B enhances doxorubicin-induced apoptosis of cancer cells but not normal cells. Biochem Pharmacol 71:584–595
Wan CK, Zhu GY, Shen XL, Chattopadhyay A, Dey S, Fong WF (2006) Gomisin A alters substrate interaction and reverses P-glycoprotein-mediated multidrug resistance in HepG2-DR cells. Biochem Pharmacol 72:824–837
Fujioka N, Kohda H, Yamasaki K, Kasai R, Tanaka O, Shoyama Y, Nishioka I (1989) Production of oleanane saponins by callus tissue of Panax japonicus. Planta Med 55:576–577
Kohda H, Namera A, Hamamoto Y (1990) Propagation of Bupleurum falcatum by shoot tip culture. Shoyakugaku Zasshi 44:38–41
Kohda H, Namera A, Koyama A, Yamazaki K, Tani T (1996) Indole alkaloid production in callus cultures of Uncaria rhynchophylla (Miq.) Miquel. Chem Pharm Bull 44:352–357
Kohda H, Kohjyouma M, Namera A, Tsuchiya H, Urimoto M, Ozaki M (1997) Production of anethole in callus of Illicium verum Hooker filius. Nat Med 51:519–522
Smiskova A, Vlasinova H, Havel L (2005) Somatic embryogenesis from zygotic embryos of Schisandra chinensis. Biol Plant 49:451–454
Brezinova L, Vlasinova H, Havel L, Humpa O, Slanina J (2010) Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application. Biomed Chromatogr 24:954–960
Opletal L, Sovová H, Bártlová M (2004) Dibenzo[a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination. J Chromatogr B 812:357–371
Avula B, Choi YW, Srinivas PV, Khan IA (2005) Quantitative determination of lignan constituents from Schisandra chinensis by liquid chromatography. Chromatographia 61:515–518
Sovova H, Opletal L, Bartlova M, Sajfrtova M, Kienkova M (2007) Supercritical fluid extraction of lignans and cinnamic acid from Schisandra chinensis. J Supercrit Fluids 42:88–95
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kohda, H., Ozaki, M. & Namera, A. Production of lignans in calluses of Schisandra chinensis . J Nat Med 66, 373–376 (2012). https://doi.org/10.1007/s11418-011-0586-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11418-011-0586-y