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Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS

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Abstract

Radix astragali (Huangqi in Chinese) is a well-known traditional Chinese medicinal herb that has been used clinically in China for centuries to cure various diseases. To profile the antiviral constituents of this herb, a high-performance liquid chromatography–diode array detector–electrospray ionization–tandem mass spectrometry (HPLC–DAD–ESI–MS/MS) analytical method was developed to separate and determinate the active part of the extract of Radix astragali, which showed potent inhibition of several viruses. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 18 compounds, comprising 11 flavonoids and 7 saponins, were identified. This study provides an approach to rapidly characterize bioactive constituents in traditional Chinese medicines (TCMs).

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Acknowledgments

This work was supported by Beijing Nova Program (2008118), 985 Project (CUN 985-03-03) and 111Project (B08044).

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Authors and Affiliations

  1. College of Life and Environmental Science, Minzu University of China, No. 27 Zhongguancun South Street, 100081, Beijing, People’s Republic of China

    Li Tang, Ying Liu, Yeling Wang & Chunlin Long

  2. Kunming Institute of Botany, Chinese Academy of Sciences, 650204, Kunming, People’s Republic of China

    Chunlin Long

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  1. Li Tang
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  2. Ying Liu
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  3. Yeling Wang
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  4. Chunlin Long
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Correspondence to Li Tang.

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Tang, L., Liu, Y., Wang, Y. et al. Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS. J Nat Med 64, 182–186 (2010). https://doi.org/10.1007/s11418-009-0381-1

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  • DOI: https://doi.org/10.1007/s11418-009-0381-1

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