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Metabolic control analysis of the Warburg-effect in proliferating vascular smooth muscle cells

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Journal of Biomedical Science

Summary

The accumulation and proliferation of vascular smooth muscle cells (VSMC) within the vessel wall is an important pathogenic feature in the development of atherosclerosis. Glucose metabolism has been implicated to play an important role in this cellular mechanism. To further elucidate the role of glucose metabolism in atherogenesis, glycolysis and its regulation have been investigated in proliferating VSMC. Platelet derived growth factor (PDGF BB)-induced proliferation of VSMCs significantly stimulated glucose flux through glycolysis. Further evaluating the enzymatic regulation of this pathway, the analysis of flux:metabolite co-responses revealed that anaerobic glycolytic flux is controlled at different sites of gycolysis in proliferating VSMCs, being consistent with the concept of multisite modulation. These findings indicate that regulation of glycolytic flux in proliferating VSMCs differs from traditional concepts of metabolic control of the Embden–Meyerhof pathway.

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Acknowledgement

This study was supported in part by a grant from the Medical Faculty of the University of Heidelberg.

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Authors and Affiliations

  1. Innere Medizin III, Universität Heidelberg, Heidelberg, Germany

    Martina Werle, Jörg Kreuzer, Julia Höfele, Albrecht Elsässer, Cordula Ackermann, Hugo A. Katus & Achim M. Vogt

  2. Praxis für Kardiologie/Pneumologie, Aachener Str. 327, D-50931, Köln, Germany

    Achim M. Vogt

Authors
  1. Martina Werle
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  2. Jörg Kreuzer
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  3. Julia Höfele
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  4. Albrecht Elsässer
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  5. Cordula Ackermann
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  6. Hugo A. Katus
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  7. Achim M. Vogt
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Correspondence to Achim M. Vogt.

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Werle, M., Kreuzer, J., Höfele, J. et al. Metabolic control analysis of the Warburg-effect in proliferating vascular smooth muscle cells. J Biomed Sci 12, 827–834 (2005). https://doi.org/10.1007/s11373-005-9010-5

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  • DOI: https://doi.org/10.1007/s11373-005-9010-5

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