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Exogenous application of carbon nanoparticles alleviates drought stress by regulating water status, chlorophyll fluorescence, osmoprotectants, and antioxidant enzyme activity in Capsicum annumn L.

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Abstract

Drought is one of the most important abiotic stresses that has a huge negative effect on crop yield. Carbon nanoparticles (CNPs) have received greater attention for their impact on the plants under abiotic stress conditions. However, it is urgently required to apply CNPs to the chili pepper (Capsicum annuum L. cv. Kaskada), which has not yet been studied. The goal of this study was to find out how CNPs affect the growth of chili pepper plants, chlorophyll pigments, proline content, and the activity of antioxidant enzymes when the plants are stressed by drought. Therefore, we synthesized and functionalized CNPs of oil fly ash by one-pot ball milling fabrication. X-ray photoelectron spectroscopy (XPS) was used to identify oxidative moieties on the CNPs surface after exposure to nitric and acetic acids. In the present study, functionalized CNPs were sprayed onto the leaves of 20-day-old plants at various concentrations (6 and 12 mg L−1) to determine their effects. We demonstrate that drought stress considerably reduces the plant height, fresh weight (FW), and dry weight (DW). Nevertheless, the exogenous application of functionalized CNPs caused an increase in relative water content (RWC), chlorophyll stability index (CSI), and chlorophyll fluorescence (Fv/Fm) under drought stress. Exogenous functionalized CNPs dramatically increased proline content under drought by reducing abscisic acid (ABA) content in the leaves. When subjected to drought stress, functionalized CNPs boosted antioxidant activities such as superoxide dismutase (SOD) and catalase (CAT) activity. Overall, the positive effects of CNPs on chili pepper seedlings open up new possibilities for developing innovative agricultural techniques, especially when plants are grown in drought conditions.

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References

  • Abdel-Motagally FMF, El-Zohri M (2018) Improvement of wheat yield grown under drought stress by boron foliar application at different growth stages. J Saudi Soc Agric Sci 17:178–185

    Google Scholar 

  • Achari GA, Kowshik M (2018) Recent developments on nanotechnology in agriculture: plant mineral nutrition, health, and interactions with soil microflora. J Agric Food Chem 66:8647–8661

    Article  CAS  Google Scholar 

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Ahmed F, Javed B, Razzaq A, Mashwani ZR (2021) Applications of copper and silver nanoparticles on wheat plants to induce drought tolerance and increase yield. IET Nanobiotechnol 15(1):68–78

  • Alabdallah NMA, Hasan MM (2021) Plant-based green synthesis of silver nanoparticles and its effective role in abiotic stress tolerance in crop plants. Saudi J Biol Sci. https://doi.org/10.1016/j.sjbs.2021.05.081

    Article  Google Scholar 

  • Alabdallah NM, Hasan MM, Hammami I, Alghamdi AI, Alshehri D, Alatawi HA (2021a) Green synthesized metal oxide nanoparticles mediate growth regulation and physiology of crop plants under drought stress. Plants 10:1730

    Article  CAS  Google Scholar 

  • Alabdallah NM, Hasan MM, Salih AM, Roushdy SS, Al-Shammari AA, Alsanie SI, El-Zaidy M (2021b) Silver nanoparticles improve growth and protect against oxidative damage in eggplant seedlings under drought stress. Plant Soil Environ 67:617–624

    Article  CAS  Google Scholar 

  • Alharbi BM, Elhakem AH, Alnusairi GSH, Soliman MH, Hakeem KR, Hasan MdM, Abdelhamid MT (2021) Exogenous application of melatonin alleviates salt stress-induced decline in growth and photosynthesis in Glycine max (L.) seedlings by improving mineral uptake, antioxidant and glyoxalase system. Plant Soil Environ 67(4):208–220

  • Ali HM, Sobze J, Pham HT, Nadeem M, Liu C, Galagedara L, Cheema M, Thomas R (2020) Carbon nanoparticles functionalized with carboxylic acid improved the germination and seedling vigor in upland boreal forest species. Nanomaterials 10:176

    Article  Google Scholar 

  • Alluqmani MS, Lolou M, Ouerfell J, Alshehri A, Salah N (2021a) Annealing effect on structural and optical properties of nanostructured carbon of oil fly ash modified titania thin-film. Results Phy 25:104335

    Article  Google Scholar 

  • Alluqmani MS, Lolou M, Ouerfell J, Alshehri A, Salah N (2021b) Elaboration of TiO2/carbon of oil fly ash nanocomposite as an eco-friendly photocatalytic thin-film material. Ceramics Int 47:13544–13551

    Article  CAS  Google Scholar 

  • Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teari D (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205e207

    Article  Google Scholar 

  • Burgess P, Huang B (2016) Mechanisms of hormone regulation for drought tolerance in plants. In: Hossain MA, Wani SH, Bhattacharjee SB, Burritt DJ, Tran LSP (eds) Drought stress tolerance in plants, Vol 1 physiology and biochemistry. Springer, New York, pp 45–76

    Chapter  Google Scholar 

  • Cao L, Meziani M, Sahu S, Sun Y (2013) Photoluminescence properties of graphene versus other carbon nanomaterials. Acc Chem Res 46:171–180

    Article  CAS  Google Scholar 

  • Chen J, Wang XJHEP (2006) Beijing: Plant physiology experimental guide. 24:55–56

  • Dash P, Dash T, Rout TK, Sahu AK, Biswal SK, Mishraa BK (2016) Preparation of graphene oxide by dry planetary ball milling process from natural graphite. RSC Adv 6:12657

    Article  CAS  Google Scholar 

  • Farhangi-Abriz S, Torabian S (2018) Nano-silicon alters antioxidant activities of soybean seedlings under salt toxicity. Protoplasma 255:953–962

    Article  CAS  Google Scholar 

  • Fincheira P, Tortella G, Duran N, Seabra AB, Rubilar O (2020) Current applications of nanotechnology to develop plant growth inducer agents as an innovation strategy. Crit Rev Biotechnol 40:15–30

    Article  CAS  Google Scholar 

  • Ge TD, Sui FG, Bai LP, Tong CL, Sun NB (2012) Effects of water stress on growth, biomass partitioning, and water-use efficiency in summer maize (Zea mays L.) throughout the growth cycle. Acta Physiol Plant 34:1043–1053

    Article  Google Scholar 

  • Giraldo JP, Landry MP, Faltermeier SM, McNicholas TP, Iverson NM, Boghossian AA, Reuel NF, Hilmer AJ, Sen F, Brew JA (2014) Plant nanobionics approach to augment photosynthesis and biochemical sensing. Nat Mater 13:400

    Article  CAS  Google Scholar 

  • Grande CA, Andreassen KA, Cavka JH, Waller D, Lorentsen OA, Øien H, Zander H-J, Poulston S, García S, Modeshia D (2018) Process intensification in nitric acid plants by catalytic oxidation of nitric oxide. Ind Eng Chem Res 57:10180–10186

    Article  CAS  Google Scholar 

  • Guo J, Song K, Wu B, Xiang Z, Zhang B, Shi Y (2017) Atomically thin SiC nanoparticles obtained via ultrasonic treatment to realize enhanced catalytic activity for the oxygen reduction reaction in both alkaline and acidic media. RSC Adv 7:22875

    Article  CAS  Google Scholar 

  • Hasan MM, Alharby HF, Hajar AS, Hakeem KR, Alzahrani Y (2019) The effect of magnetized water on the growth and physiological conditions of Moringa species under drought stress. Pol J Environ Stud 28:1145–1155

    Article  Google Scholar 

  • Hasan MM, Alharby HF, Uddin MN, Ali MA, Anwar Y, Fang XW, Hakeem KR, Alzahrani Y, Hajar AS (2020a) Magnetized water confers drought stress tolerance in Moringa biotype via modulation of growth, gas exchange, lipid peroxidation and antioxidant activity. Pol J Environ Stud 1:29

    Google Scholar 

  • Hasan MM, Ali MA, Soliman MH, Alqarawi AA, Abd Allah EF, Fang XW (2020b) Insights into 28-homobrassinolide (HBR)- mediated redox homeostasis, AsA–GSH cycle, and methylglyoxal detoxification in soybean under drought-induced oxidative stress. J Plant Inter 15:371–385

    CAS  Google Scholar 

  • Hasan MM, Skalicky M, Jahan MS, Hossain MN, Anwar Z, Nie ZF, Alabdallah NM, Brestic M, Hejnak V, Fang XW (2021a) Spermine: its emerging role in regulating drought stress responses in plants. Cells 261:1–15

    Google Scholar 

  • Hasan MM, Alabdallah NM, Alharbi BM, Waseem M, Yao G, Liu XD, Abd El-Gawad HG, El-Yazied AA, Ibrahim MFM, Jahan MS, Fang XW (2021b) GABA: a key player in drought stress resistance in plants. Int J Mol Sci 22:10136

    Article  CAS  Google Scholar 

  • Hasan MM, Gong L, Nie Z, Feng X, Ahammed GJ, Fang XW (2021c) ABA-induced stomatal movements in vascular plants during dehydration versus rehydration. Environ Exp Bot 186:104436

    Article  CAS  Google Scholar 

  • Hasan MM, Alharbi BM, Alhaithloul HAS, Abdulmajeed AM, Alghanem SM, Al-Mushhin AAM, Jahan MS, Corpas FJ, Fang X-W, Soliman MH (2021d) Spermine-mediated tolerance to selenium toxicity in wheat (Triticum aestivum L.) depends on endogenous nitric oxide synthesis. Antioxidants 10:1835

    Article  CAS  Google Scholar 

  • Hossain MA, Hoque MA, Burrit DJ, Fujita M (2014) Proline protects plants against abiotic oxidative stress: biochemical and molecular mechanisms. In: Ahmad P (ed) Oxidative damage to plants. Academic press, Cambridge, pp 477–522

    Chapter  Google Scholar 

  • Hussain HA, Men S, Hussain S, Chen Y, Ali S, Zhang S, Zhang K, Li Y, Xu K, Liao C, Wang L (2019) Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Sci Rep 9:1–12

    Article  Google Scholar 

  • Hussain HA, Men S, Hussain S, Zhang Q, Ashraf U, Anjum SA, Ali I, Wang L (2020) Maize tolerance against drought and chilling stresses varied with root morphology and antioxidative defense system. Plants 9:720

    Article  CAS  Google Scholar 

  • Jahan MS, Guo S, Sun J, Shu S, Wang Y, El-Yazied AA, Alabdallah NM, Hikal M, Mohamed MHM, Ibrahim MFM, Hasan MM (2021a) Melatonin-mediated photosynthetic performance of tomato seedlings under high-temperature stress. Plant Physiol Biochem 167:309–320

    Article  CAS  Google Scholar 

  • Jahan MS, Shu S, Wang Y, Hasan M, El-Yazied AA, Alabdallah NM, Hajjar D, Altaf MA, Sun J, Guo S (2021b) Melatonin pretreatment confers heat tolerance and repression of heat-induced senescence in tomato through the modulation of ABA and GA-mediated pathways. Front Plant Sci 12:381

    Article  Google Scholar 

  • Khan A, Anwar Y, Hasan M, Iqbal A, Ali M, Alharby HF, Hakeem KR, Hasanuzzaman M (2017) Attenuation of drought stress in Brassica seedlings with exogenous application of Ca2+ and H2O2. Plants 6:20

    Article  Google Scholar 

  • Khodakovskaya MV, De Silva K, Biris AS, Dervishi E, Villagarcia H (2012) Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano 6:2128–2135

    Article  CAS  Google Scholar 

  • Khodakovskaya MV, Kim BS, Kim JN, Alimohammadi M, Dervishi E, Mustafa T, Cernigla CE (2013) Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small 9:115–123

    Article  CAS  Google Scholar 

  • Kumar M, Xiong X, Wan Z, Sun Y, Tsang DCW, Gupta J et al (2020) Ball milling as a mechanochemical technology for fabrication of novel biochar nanomaterials. Bioresource Technol 312:123613

    Article  CAS  Google Scholar 

  • LeCroy EG, Yang ST, Yang F, Liu Y, Fernando SAK, Bunker EC, Luo GP, Sun YP (2015) Functionalized carbon nanoparticles: syntheses and applications in optical bioimaging and energy conversion. Coord Chem Rev 320–321:66–81

    Google Scholar 

  • Liu R, Lal R (2015) Potentials of engineered nanoparticles as fertilizers for increasing agronomic productions. Sci Total Environ 514:131–139

    Article  CAS  Google Scholar 

  • López-Vargas ER, González-García Y, Pérez-Álvarez M, Cadenas-Pliego G, González-Morales S, Benavides-Mendoza A, Cabrera RI, Juárez-Maldonado A (2020) Seed priming with carbon nanomaterials to modify the germination, growth, and antioxidant status of tomato seedlings. Agron 10:639

    Article  Google Scholar 

  • Meng LY (2012) Preparation and characterization of reduced graphene nanosheets via pre-exfoliation of graphite flakes. Bull Korean Chem Soc 33:209

    Article  CAS  Google Scholar 

  • Mohammadzadeh M, Bagheri H, Ghader S (2020) Study on extraction and separation of Ni and Zn using [bmim][PF6] IL as selective extractant from nitric acid solution obtained from zinc plant residue leaching. Arabian J Chem 13:5821–5831

    Article  CAS  Google Scholar 

  • Mukherjee S, Choudhuri MJP (1983) Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plantarum 58:166–170

    Article  CAS  Google Scholar 

  • Mukherjee A, Majumdar S, Servin AD, Pagano L, Dhankher OP, White JC (2016) Carbon nanomaterials in agriculture: a critical review. Front Plant Sci 7:172

    Article  Google Scholar 

  • Müller M, Munné-Bosch S (2011) Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Plant Methods 7:37

    Article  Google Scholar 

  • Murphy KS (1962) Modifications of the technique for determination of chlorophyll stability index in relation to studies of drought resistance in rice. Curr Sci 31:470–471

    Google Scholar 

  • Ndao AS, Konté A, Biaye M, Faye ME, Faye NAB, Wagu A (2005) Analysis of chlorophyll fluorescence spectra in some tropical plants. J Fluoresc 15:123–129

    Article  CAS  Google Scholar 

  • Panova GG, Ktitorova IN, Skobeleva OV, Sinjavina NG, Charykov NA, Semenov KN (2016) Impact of polyhydroxy fullerene (fullerol or fullerenol) on growth and biophysical characteristics of barley seedlings in favourable and stressful conditions. Plant Growth Regul 79:309–317

    Article  CAS  Google Scholar 

  • Sahitya UL, Krishna MSR, Suneetha P (2019) Integrated approaches to study the drought tolerance mechanism in hot pepper (Capsicum annuum L.). Physiol Mol Biol Plants 25:637–647

    Article  CAS  Google Scholar 

  • Salah N, Habib SS, Khan ZH, Alshahrie A, Memic A, Al-Gamdi AA (2016) Carbon rich fly ash and their nanostructures. Carbon Lett 19:23–31

    Article  Google Scholar 

  • Schachtman DP, Goodger JQD (2008) Chemical root to shoot signaling under drought. Trends Plant Sci 13:281–287

    Article  CAS  Google Scholar 

  • Shekhawat GS, Mahawar L, Rajput P, Rajput VD, Minkina T, Singh RK (2021) Role of engineered carbon nanoparticles (CNPs) in promoting growth and metabolism of Vigna radiata (L.) Wilczek: insights into the biochemical and physiological responses. Plants 10:1317

    Article  CAS  Google Scholar 

  • Sohag AAM, Tahjib-Ul-Arif M, Brestič M, Afrin S, Sakil MA, Hossain MT, Hossain MA, Hossain MA (2020) Exogenous salicylic acid and hydrogen peroxide attenuates drought stress in rice. Plant Soil Environ 66:7–13

    Article  Google Scholar 

  • Talaat NB, Shawky BT, Ibrahim AS (2015) Alleviation of drought-induced oxidative stress in maize (Zea mays L.) plants by dual application of 24-epibrassinolide and spermine. Environ Exp Bot 113:47–58

    Article  CAS  Google Scholar 

  • Tiwari D, Dasgupta-Schubert N, Cendejas LV, Villegas J, Montoya LC, García SB (2014) Interfacing carbon nanotubes (CNT) with plants: enhancement of growth, water and ionic nutrient uptake in maize (Zea mays) and implications for nano-agriculture. Appl Nano Sci 4:577–591

    Article  CAS  Google Scholar 

  • Usman M, Farooq M, Wakeel A, Nawaz A, Cheema SA, Rehman ur H, Ashraf I, Sanaullah M (2020) Nanotechnology in agriculture: current status, challenges and future opportunities. Sci Total Environ 721:137778

    Article  CAS  Google Scholar 

  • Villagarcia H, Dervishi E, de Silva K, Biris AS, Khodakovskaya MV (2012) Surface chemistry of carbon nanotubes impacts the growth and expression of water channel protein in tomato plants. Small 8:2328–2334

    Article  CAS  Google Scholar 

  • Walters SA, Jha AK (2016) Sustaining chili pepper production in Afghanistan through better irrigation practices and management. Agric 6:62

    Google Scholar 

  • Wang H, Zhang M, Song Y, Li H, Huang H, Shao M, Liu Y, Kang Z (2018) Carbon dots promote the growth and photosynthesis of mung bean sprouts. Carbon 136:94–102

    Article  CAS  Google Scholar 

  • Xin X, Zhao F, Zhao H, Goodrich SL, Hill MR, Sumerlin BS, Stoffella PJ, Wright AL, He Z (2020) Comparative assessment of polymeric and other nanoparticles impacts on soil microbial and biochemical properties. Geoderma 367:114278

    Article  CAS  Google Scholar 

  • Zhao CM, Wang GX, Wei XP, Deng JM, Cheng DL (2007) Effects of groundwater depth variation on photosynthesis and photoprotection of Elaeagnus angustifolia L. Trees 21:55–63

    Article  CAS  Google Scholar 

  • Zhao X, Chen H, Kong F, Zhang Y, Wang S, Liu S et al (2019) Fabrication, characteristics and applications of carbon materials with different morphologies and porous structures produced from wood liquefaction: a review. Chem Eng J 364:226–243

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physics, Faculty of Applied Science, Umm Al-Qura University, Makkah, 21955, Saudi Arabia

    Saleh M. Alluqmani

  2. Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia

    Nadiyah M. Alabdallah

  3. Basic and Applied Scientific Research Centre, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia

    Nadiyah M. Alabdallah

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S.M. Alluqmani: conceptualization, methodology, data curation, formal analysis, software, writing—original draft, writing—review and editing; N.M. Alabdallah: methodology, supervision, conceptualization, methodology, writing—review and editing.

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Correspondence to Nadiyah M. Alabdallah.

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Alluqmani, S.M., Alabdallah, N.M. Exogenous application of carbon nanoparticles alleviates drought stress by regulating water status, chlorophyll fluorescence, osmoprotectants, and antioxidant enzyme activity in Capsicum annumn L.. Environ Sci Pollut Res 30, 57423–57433 (2023). https://doi.org/10.1007/s11356-023-26606-0

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