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Competent antioxidant and antiglycation properties of zinc oxide nanoparticles (ZnO-NPs) phyto-fabricated from aqueous leaf extract of Boerhaavia erecta L.

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Abstract

During the present century, plant-based zinc oxide nanoparticles (ZnO-NPs) are exploited extensively for their vast biological properties due to their unique characteristic features and eco-friendly nature. Diabetes is one of the fast-growing human diseases/abnormalities worldwide, and the need for new/ novel antiglycation products is the need of the hour. The study deals with the phyto-fabrication of ZnO-NPs from Boerhaavia erecta, a medicinally important plant, and to evaluate their antioxidant and antiglycation ability in vitro. UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) were used to characterize the phyto-fabricated ZnO-NPs. The characterization of nanoparticles revealed that the particles showed an absorption peak at 362 nm and band gap energy of 3.2 eV, approximately 20.55 nm in size, with a ZnO elemental purity of 96.61%. The synthesized particles were found agglomerated when observed under SEM, and the FT-IR studies proved that the phyto-constituents of the extract involved during the different stages (reduction, capping, and stabilization) of nanoparticles synthesis. The antioxidant and metal chelating activities confirmed that ZnO-NPs could inhibit the free radicals generated, which was dose-dependent with an IC50 value between 1.81 and 1.94 mg mL–1, respectively. In addition, the phyto-fabricated nanoparticles blocked the formation of advanced glycation end products (AGEs) as noticed through inhibition of Amadori products, trapping of reactive dicarbonyl intermediate and breaking the cross-link of glycated protein. It was also noted that the phyto-fabricated ZnO-NPs significantly prevented the damage of red blood corpuscles (RBCs) induced by MGO. The present study’s findings will provide an experimental basis for exploring ZnO-NPs in diabetes-related complications.

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Acknowledgements

The authors thank the Department of Studies in Botany, Biotechnology and Microbiology of the University of Mysore, Mysuru, for providing facilities to carry out research. The authors would also like to thank the University with Potential of Excellence (UPE) Authorities University of Mysore, Mysuru, for instrumentation facilities.

Funding

The author M. Murali likes to acknowledge the University Grants Commission (UGC), New Delhi, India, for providing financial support by awarding a Post-Doctoral Fellowship (No. F/PDFSS-2015-17-KAR-11846).

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Authors and Affiliations

  1. Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India

    Mahadevamurthy Murali & Sudarshana Brijesh Singh

  2. Department of Clinical Nutrition and Dietetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, 563101, Karnataka, India

    Anjana Thampy & Satish Anandan

  3. Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India

    Mohammed Aiyaz

  4. Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India

    Natarajamurthy Shilpa

  5. Department of PG Studies in Biotechnology, Nrupathunga University, Nrupathunga Road, Bangalore, 560001, India

    Hittanahallikoppal Gajendramurthy Gowtham

  6. Department of Studies in Environmental Science, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India

    Abhilash Mavinakere Ramesh

  7. Department of Physics, Faculty of Science, University of Zabol, Zabol, 98613-35856, Iran

    Abbas Rahdar

  8. Department of Chemistry, International Hellenic University, Kavala, Greece

    George Z. Kyzas

Authors
  1. Mahadevamurthy Murali
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  2. Anjana Thampy
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  10. George Z. Kyzas
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Contributions

All authors contributed to the study conception and design. The authors indicated in parentheses made substantial contributions to the following tasks of research: conceptualization (Mahadevamurthy Murali, Anjana Thampy, Satish Anandan, Ashween Bilagi, Mohammed Aiyaz, Natarajamurthy Shilpa, Sudarshana Brijesh Singh, Hittanahallikoppal Gajendramurthy Gowtham, Abhilash Mavinakere Ramesh, Abbas Rahdar, George Z. Kyzas); writing-original draft, writing-revised, investigation, and methodology (Mahadevamurthy Murali, Anjana Thampy, Satish Anandan, Mohammed Aiyaz, Natarajamurthy Shilpa, Sudarshana Brijesh Singh, Hittanahallikoppal Gajendramurthy Gowtham, Abhilash Mavinakere Ramesh, Abbas Rahdar, George Z. Kyzas); and supervision (Satish Anandan). All authors read and approved the final manuscript.

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Correspondence to Satish Anandan.

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Murali, M., Thampy, A., Anandan, S. et al. Competent antioxidant and antiglycation properties of zinc oxide nanoparticles (ZnO-NPs) phyto-fabricated from aqueous leaf extract of Boerhaavia erecta L.. Environ Sci Pollut Res 30, 56731–56742 (2023). https://doi.org/10.1007/s11356-023-26331-8

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