Abstract
The protective mechanism of extracellular polymeric substance (EPS) secreted by a harmful cyanobacteria against tannins allelochemicals was explored in this study. The binding properties of soluble EPS (SEPS) and bound EPS (BEPS) of Microcystis aeruginosa to tannic acid (TA) were investigated via fluorescence spectroscopy. The results suggested that TA interacted with the proteins in SEPS and BEPS mainly with binding constants of 5.26 and 7.93 L/mol, respectively; TA interacted with the humic acids in SEPS and BEPS mainly with binding constants of 5.12 and 5.24 L/mol, respectively. Thermodynamic experiments confirmed that the binding was mainly controlled by the hydrophobic force. Combined with Fourier transform infrared spectroscopy, it was found that the amine, carbonyl, carboxyl, and hydroxyl groups in EPS were the main functional groups contributing to the interaction of TA with EPS. The existence of EPS reduced the toxicity of TA to algal cells, with the 96 h inhibition rate of 40 mg L−1 TA on algal cells decreasing by 48.95%. The results of this study may improve our understanding of the protective mechanism of cyanobacteria against tannins allelochemicals.
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The datasets analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the National Natural Science Foundation of China (Grant No. 51979137, 51779079, and 41931292).
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All persons who have made substantial contributions to the work were mentioned in this statement. In this manuscript, Shiyin Li and Lixiao Ni have acquisition of the financial support for this research and provided conceptualization ideas for this study. Li Yin has completed the overall experimental design and operation and drafted the manuscript. Juan Wang and Kaipian Shi participated in some experimental operations and analyzed study data. Yong Zhang, Ying Xu, and Desheng Kong contributed to the interpretation of the results and critical revision of the manuscript.
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Yin, L., Wang, J., Shi, K. et al. Interactions between tannins allelochemicals and extracellular polymeric substance (EPS) of Microcystis aeruginosa. Environ Sci Pollut Res 29, 83211–83219 (2022). https://doi.org/10.1007/s11356-022-21661-5
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DOI: https://doi.org/10.1007/s11356-022-21661-5