Abstract
Nanotechnology tends to be a swiftly growing field of research that actively influences and inhibits the growth of bacteria/cancer. Noble metal nanoparticles (NPs) such as silver, copper, and gold have been used to damage bacterial and cancer growth over recent years; however, the toxicity of higher NPs concentrations remains a major issue. The copper oxide nanoparticles (CuONPs) were therefore fabricated using a simple green chemistry approach. Biofabricated CuONPs were characterized using UV-visible, FE-SEM with EDS, HR-TEM, FT-IR, XRD, Raman spectroscopy, and XPS analysis. Formations of CuONPs have been observed by UV-visible absorbance peak at 360.74 nm. The surface morphology of the CuONPs showed the spherical structure and size (~ 68 nm). The EDS spectrum of CuONPs has proved to be the key signals of copper (Cu) and oxygen (O) components. FT-IR analysis, to validate the important functional biomolecules (O–H, C=C, C–H, C–O) are responsible for reduction and stabilization of CuONPs. The monoclinic end-centered crystalline structures of CuONPs were confirmed with XRD planes. The electrochemical oxygen states of the CuONPs have been studied using spectroscopy of the Raman and X-ray photoelectron. After successful preparation, CuONPs examined their antibacterial, anticancer, and photocatalytic activities. Green-fabricated CuONPs were promising antibacterial candidate against human pathogenic gram-negative bacteria Escherichia coli, Vibrio cholerae, Salmonella typhimurium, Klebsiella pneumoniae, Aeromonas hydrophila, and Pseudomonas aeruginosa. CuONPs were demonstrated the excellent anticancer activity against A549 human lung adenocarcinoma cell line. Furthermore, CuONPs exhibited photocatalytic degradation of azo dyes such as eosin yellow (EY), rhodamine 123 (Rh 123), and methylene blue (MB). Biofabricated CuONPs may therefore be an important biomedical research for the aid of bacterial/cancer diseases and photocatalytic degradation of azo dyes.
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The first author Dinesh Babu Manikandan is grateful to the Bharathidasan University for having received the University Research Fellowship (Ref. No. 03534/URF/K7/2016 dt: 09.03.2016). The authors wholeheartedly thank the UGC-SAP-DRS-II (F.3-9/2013[SAP-II], Department of Science and Technology-Fund for Improvement of Science and Technology Infrastructure (DST-FIST)-Level-I (stage-II) (Ref. No. SR/FST/LSI-647/2015(C) Date.11.08.2016) and Department of Science and Technology Promotion of University Research and Scientific Excellence (DST PURSE Phase—II) (Ref. No. SR/PURSE PHASE 2/16(G) /& 16(C) Date. 21.02.2017) of the Department of Animal Science, Bharathidasan University for the instrumentation facility and National Centre for Alternatives to Animal Experiments (NCAAE) under UGC-CPEPA scheme, Government of India (F.No.2-1/2013(NS/PE)). The authors also thank “RUSA, 2.0 - Biological Sciences, Bharathidasan University”. The author Srinivasan Veeran would like to thank DST-SERB for the award of N-PDF (File No. PDF/2016/003501, dt: 28.03.2017).
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Dinesh Babu Manikandan: investigation, methodology, writing—original draft, writing—review and editing. Manikandan Arumugam: investigation, resources. Srinivasan Veeran: formal analysis, data curation. Arun Sridhar: investigation, data curation, writing—review and editing. Rajkumar Krishnasamy Sekar: formal analysis, data curation. Balaji Perumalsamy: methodology, investigation, resources. Thirumurugan Ramasamy: conceptualization, project administration, supervision, validation, writing—review and editing.
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Manikandan, D.B., Arumugam, M., Veeran, S. et al. Biofabrication of ecofriendly copper oxide nanoparticles using Ocimum americanum aqueous leaf extract: analysis of in vitro antibacterial, anticancer, and photocatalytic activities. Environ Sci Pollut Res 28, 33927–33941 (2021). https://doi.org/10.1007/s11356-020-12108-w
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DOI: https://doi.org/10.1007/s11356-020-12108-w