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Abatement of arsenic-induced phytotoxic effects in rice seedlings by an arsenic-resistant Pantoea dispersa strain

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Abstract

Population detonation and rapid industrialization are the major factors behind the reduction in cultivable land that affects crop production seriously. This situation is further being deteriorated due to the negative effects of abiotic stresses. Under such conditions, plant growth-promoting rhizobacteria (PGPR) are found to improve crop production which is essential for sustainable agriculture. This study is focused on the isolation of potent arsenic (As)-resistant PGPR from the agricultural land of West Bengal, India, and its application to reduce As translocation in rice seedlings. After screening, an As-resistant PGPR strain AS18 was identified by phenotypic characters and 16S rDNA sequence-based homology as Pantoea dispersa. This strain displayed nitrogen fixation, phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) activity, indole-3-acetic acid (IAA) production, in addition to As (III) resistance up to 3750 μg/mL. The As removal efficiency of this strain was up to 93.12% from the culture medium as evidenced by AAS. The bioaccumulation property of AS18 strain was further validated by TEM-EDAX-XRD-XRF-FTIR studies. This strain showed significant morpho-biochemical improvements including antioxidant enzymatic activities and As-minimization in plant (rice) cells. Thus, being an As-resistant potent PGPR, AS18 strain is expected to be applied in As-spiked agricultural fields for bioremediation and phytostimulation.

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Data availability

All data generated or analyzed during this study are included in this published article (and its supplementary information files). The 16S rDNA sequence of Pantoea dispersa strain AS18 is available in NCBI database vide accession number—MH605572 and the strain is available at NCMR, NCCS, Pune, India, with strain accession—MCC 4044.

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Funding

The first author is thankful to the University of Burdwan, West Bengal, India, for awarding the institutional fellowship (No.: 2018/75). The authors are also gratefully acknowledged to Council of Scientific and Industrial Research (CSIR), India, for financial support for this work [vide letter No. 38 (1469)/18/EMR-II, dt.04.04.18]. KP gratefully acknowledges the financial assistance from the University Grants Commission (UGC), New Delhi, for UGC—Dr. D. S. Kothari Post-Doctoral Fellowship [No.F.4-2/2006 (BSR)/BL/19-20/0072 dated October 21, 2019].

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Authors and Affiliations

  1. Microbiology Laboratory, Department of Botany, The University of Burdwan, Purba Bardhaman, West Bengal, 713104, India

    Antara Ghosh, Sayanta Mondal, Sudip Kumar Ghosh & Tushar Kanti Maiti

  2. Mycology and Plant Pathology Laboratory, Department of Botany, Siksha Bhavana, Visva-Bharati, Santiniketan, Birbhum, West Bengal, 731235, India

    Krishnendu Pramanik

  3. Nanospinics Laboratory, Department of Materials Science & Engineering, Seoul National University, Seoul, 151-744, South Korea

    Shatabda Bhattacharya

  4. DODL, University of Kalyani, Nadia, Kalyani, West Bengal, 741235, India

    Pallab Kumar Ghosh

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  1. Antara Ghosh
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  2. Krishnendu Pramanik
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  3. Shatabda Bhattacharya
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  4. Sayanta Mondal
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  5. Sudip Kumar Ghosh
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  6. Pallab Kumar Ghosh
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  7. Tushar Kanti Maiti
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Contributions

AG performed most of the experiments starting from soil sample collection, isolation, screening, and characterization of rhizobacterial strain; perfomed plant growth promotion experiments; and analyzed and interpreted the data including writing the first draft of the manuscript. KP has monitored antioxidant enzymes of rice seedlings, performed the statististical analysis, prepared the phylogenetic tree of AS18 strain, and analyzed and interpreted the data including the revision and editing of the MS. SB has contributed to all the experiments related to As bioaccumulation of AS18 strain (TEM, EDAX, XRD, FTIR and AAS) except XRF analysis, AAS analysis of rice seedlings, analyzed and interpreted the data including the revision of the MS. SM performed XRF analysis of AS18 strain as well as revised the MS. SKG has performed the viability test of AS18 strain. PKG has performed the qualititative test for As oxidation-reduction experiment of AS18 strain. TKM has contributed to the experimental conception, design, supervision, validation of the data editing, and revision of the MS. All authors read and approved the final manuscript.

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Correspondence to Tushar Kanti Maiti.

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Ghosh, A., Pramanik, K., Bhattacharya, S. et al. Abatement of arsenic-induced phytotoxic effects in rice seedlings by an arsenic-resistant Pantoea dispersa strain. Environ Sci Pollut Res 28, 21633–21649 (2021). https://doi.org/10.1007/s11356-020-11816-7

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