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DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills

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Abstract

Cyprodinil and thiacloprid are two of the most commonly used pesticides in Turkey. It is more likely to reach humans or animals due to their widespread use. This study aims to investigate whether there is a DNA damage risk due to cyprodinil and thiacloprid exposure. Zebrafish, which is used as a model organism in health and environmental research, and comet assay were chosen to demonstrate this damage. Ten zebrafish per group were exposed to 2 different concentrations for each pesticides (0.31 and 0.155 mg/L for cyprodinil and 1.64 and 0.82 mg/L for thiacloprid) for 21 days. After, gills were excised and comet assay was performed. Photos of an average of 50 cells per slide were taken and were analyzed with visual evaluation program. DNA damage was found to be increased in the 0.31 mg/L cyprodinil, 0.82 mg/L thiacloprid, and 1.64 mg/L thiacloprid treatment groups when compared to the control group (p < 0.001). Average tail DNA percentage parameter values were 9.45 ± 0.51, 10.30 ± 0.34, 11.17 ± 0.33, and 2.47 ± 0.06 respectively. Cyprodinil and thiacloprid were identified as genotoxic agents that should be investigated further.

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Funding

This work was supported by Süleyman Demirel University Scientific Research Projects Coordination Unit with the project number FYL-2018-6695.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

    Vehbi Atahan Toğay, Gülçin Yavuz Türel, Dilek Aşcı Çelik & Eda Evgen Tülüceoğlu

  2. Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

    Meltem Özgöçmen

  3. Department of Biomedical Engineering, Faculty of Technology, Isparta University of Applied Sciences, Isparta, Turkey

    İsmail Şen

  4. Department of Biology, Faculty of Art and Science, Süleyman Demirel University, Isparta, Turkey

    Yusuf Ayvaz

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  1. Vehbi Atahan Toğay
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Correspondence to Vehbi Atahan Toğay.

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Toğay, V., Yavuz Türel, G., Aşcı Çelik, D. et al. DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills. Environ Sci Pollut Res 28, 14482–14487 (2021). https://doi.org/10.1007/s11356-020-11668-1

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  • DOI: https://doi.org/10.1007/s11356-020-11668-1

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