Skip to main content
SpringerLink
Log in

Talaromyces pinophilus strain M13: a portrayal of novel groundbreaking fungal strain for phytointensification

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The aim of current research was to explore different fungi as plant growth promoting fungi (PGPF). Strains of Trichoderma are well explored till now. But there are few other fungal strains that are better than Trichoderma strains. The study involves the isolation of different fungi from the rhizosphere of various agriculture farms. After isolation, 18S rRNA identification was carried out. Isolated fungi belonged to genus Penicillium, Talaromyces, Trichoderma, and Aspergillus. Isolate M13 belonging to genus Talaromyces was screened for its plant growth promoting (PGP) activity as it is a novel strain and still to be explored. Isolate M13 was identified as Talaromyces pinophilus (MG011365). Indole acetic acid (IAA) estimation was carried out using Salkowski reagent. Isolate was allowed to grow in cultivation media (Potato Dextrose Broth, PDB) in which one was supplemented with tryptophan (TRP) and one was without TRP. Phosphate solubilization was assessed using Pikovskaya’s media and latter estimated using stannous chloride method, showing decent solubilization of phosphate. Siderophore production was assessed using CAS assay that indicated decent extent of siderophore production. Further for biocontrol, enzymatic assay for β-glucanase and chitinase was carried out. For β-glucanase enzyme production 1% carboxymethylcellulose (CMC) and for chitinase enzyme production 10% v/v colloidal chitin (as a sole carbon source) supplemented in solid minimal-medium-9 (MM9) were used. Antagonism effect of isolate M13 was carried out against Aspergillus niger by dual cultural method. As the strain M13 showed several PGP traits, liquid bioformulation was prepared to perform seed germination assay and pot trials on chickpea, followed by field trial on banana plant. All the experimental data of biochemical assessment with pot and field trials suggest T. pinophilus M13 as a novel fungus that can be used as biointensifier.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Adhikari P, Pandey A (2019) Phosphate solubilization potential of endophytic fungi isolated from Taxus wallichiana Zucc roots. Rhizosphere 9:2–9

    Article  Google Scholar 

  • Ali W, Nadeem M, Ashiq W, Zaeem M, Rajabi-Khamseh GSSM, S. & Cheema M. (2019) The effects of organic and inorganic phosphorus amendments on the biochemical attributes and active microbial population of agriculture podzols following silage corn cultivation in boreal climate. Sci Rep 9(1):1–17

    Article  Google Scholar 

  • Al-Taie AH, Al-Zubaidi NK, Javaid A (2019) Evaluation of application methods of plant growth promoting fungi (PGPF) on plant attributes of wheat. BioCell 43(6)

  • Anam GB, Reddy MS, Ahn YH (2019) Characterization of Trichoderma asperellum RM-28 for its sodic/saline-alkali tolerance and plant growth promoting activities to alleviate toxicity of red mud. Sci Total Environ 662:462–469

    Article  CAS  Google Scholar 

  • Anilkumar RR, Edison LK, Pradeep NS (2017) Exploitation of fungi and actinobacteria for sustainable agriculture. Microb. Biotechnol. Springer, Singapore, pp 135–162

    Google Scholar 

  • Arya GC, Harel A (2019) Phyllosphere and its potential role in sustainable agriculture. Microbial genomics in sustainable agroecosystems. Springer, Singapore, pp 39–65

    Book  Google Scholar 

  • Bang C, Dagan T, Deines P, Dubilier N, Duschl WJ, Fraune S, Picazo D (2018) Metaorganisms in extreme environments: do microbes play a role in organismal adaptation. J Zool 127:1–19

    Article  Google Scholar 

  • Bernardi AV, de Gouvêa PF, Gerolamo LE, Yonamine DK, de Lima Balico LDL, Uyemura SA, Dinamarco TM (2018) Functional characterization of GH7 endo-1, 4-β-glucanase from Aspergillus fumigatus and its potential industrial application. Protein Expr Purif 150:1–11

    Article  CAS  Google Scholar 

  • Di Cologna NDM, Gómez-Mendoza DP, Zanoelo FF, Giannesi GC, de Alencar Guimaraes NC, de Souza Moreira LR, Ricart CAO (2018) Exploring Trichoderma and Aspergillus secretomes: proteomics approaches for the identification of enzymes of biotechnological interest. Enzym Microb Technol 109:1–10

    Article  Google Scholar 

  • Doni F, Zain CRCM, Isahak A, Fathurrahman F, Anhar A, Mohamad WNAW, Uphoff N (2018) A simple, efficient, and farmer-friendly Trichoderma-based biofertilizer evaluated with the SRI Rice Management System. Organic Agriculture 8(3):207–223

  • Du C, Jiang S, Jiang S, Zhou Y, Zhang G (2019) A Bacillus pumilus originated β-N-acetylglucosaminidase for chitin combinatory hydrolysis and exploration of its thermostable mechanism. Int J Biol Macromol 132:1282–1289

    Article  CAS  Google Scholar 

  • Etesami H (2018) Bacterial mediated alleviation of heavy metal stress and decreased accumulation of metals in plant tissues: mechanisms and future prospects. Ecotoxicol Environ Saf 147:175–191

    Article  CAS  Google Scholar 

  • Fujii T, Inoue H, Yano S, Sawayama S (2018) Strain improvement for industrial production of lignocellulolytic enzyme by Talaromyces cellulolyticus. Fungal Cellulolytic Enzymes. Springer, Singapore, pp 135–154

    Google Scholar 

  • Gilliam FS, Burns DA, Driscoll CT, Frey SD, Lovett GM, Watmough SA (2019) Decreased atmospheric nitrogen deposition in eastern North America: predicted responses of forest ecosystems. Environ Pollut 244:560–574

    Article  CAS  Google Scholar 

  • Goswami D, Pithwa S, Dhandhukia P, Thakker JN (2014) Delineating Kocuria turfanensis 2M4 as a credible PGPR: a novel IAA-producing bacteria isolated from saline desert. J Plant Interact 9(1):566–576

    Article  CAS  Google Scholar 

  • Goswami D, Patel K, Parmar S, Vaghela H, Muley N, Dhandhukia P, Thakker JN (2015) Elucidating multifaceted urease producing marine Pseudomonas aeruginosa BG as a cogent PGPR and bio-control agent. Plant Growth Regul 75(1):253–263

    Article  CAS  Google Scholar 

  • Harish S, Parthasarath S, Durgadevi D, Anandhi K, Raguchander T (2019) Plant growth-promoting rhizobacteria: harnessing its potential for sustainable plant disease management. In: Plant growth promoting rhizobacteria for agricultural sustainability. Springer, Singapore, pp 151–187

    Chapter  Google Scholar 

  • Jaber LR, Ownley BH (2018) Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens? Biol Control 116:36–45

    Article  Google Scholar 

  • Kalia A, Kaur H (2019) Nano-biofertilizers: harnessing dual benefits of nano-nutrient and bio-fertilizers for enhanced nutrient use efficiency and sustainable productivity. In: Nanoscience for sustainable agriculture. Springer, Cham, pp 51–73

    Chapter  Google Scholar 

  • Kolachevskaya OO, Lomin SN, Arkhipov DV, Romanov GA (2019) Auxins in potato: molecular aspects and emerging roles in tuber formation and stress resistance. Plant Cell Rep:1–18

  • Kumar P, Thakur S, Dhingra GK, Singh A, Pal MK, Harshvardhan K, Maheshwari DK (2018) Inoculation of siderophore producing rhizobacteria and their consortium for growth enhancement of wheat plant. Biocatal Agric Biotechnol 15:264–269

    Article  Google Scholar 

  • Kun RS, Gomes ACS, Hildén KS, Cerezo SS, Mäkelä MR, de-Vries RP. (2019) Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation. Biotechnol Adv 37:107361

    Article  CAS  Google Scholar 

  • Mahmood A, Iguchi R, Kataoka R (2019a) Multifunctional food waste fertilizer having the capability of Fusarium-growth inhibition and phosphate solubility: a new horizon of food waste recycle using microorganisms. Waste Manag 94:77–84

    Article  CAS  Google Scholar 

  • Mahmood A, Kataoka R, Turgay OC, Yaprak AE (2019b) Halophytic microbiome in ameliorating the stress, Ecophysiology, abiotic stress responses and utilization of halophytes. Springer, Singapore, pp 171–194

    Google Scholar 

  • Mehta P, Sharma R, Putatunda C, Walia A (2019) Endophytic fungi: role in phosphate solubilization, Advances in endophytic fungal research. Springer, Cham., pp 183–209

    Book  Google Scholar 

  • Minden V, Olde Venterink H (2019) Plant traits and species interactions along gradients of N, P and K availabilities. Funct Ecol 33(9):1611–1626

    Article  Google Scholar 

  • Mohamed MF, Thalooth AT, Elewa TA, Ahmed AG (2019) Yield and nutrient status of wheat plants (Triticum aestivum) as affected by sludge, compost, and biofertilizers under newly reclaimed soil. Bull Natl Res Centre 43(1):31

    Article  Google Scholar 

  • Moreno-Velandia CA, Izquierdo-García LF, Ongena M, Kloepper JW, Cotes AM (2019) Soil sterilization, pathogen and antagonist concentration affect biological control of Fusarium wilt of cape gooseberry by Bacillus velezensis Bs006. Plant Soil 435(1-2):39–55

    Article  CAS  Google Scholar 

  • Numan M, Bashir S, Khan Y, Mumtaz R, Shinwari ZK, Khan AL, Ahmed AH (2018) Plant growth promoting bacteria as an alternative strategy for salt tolerance in plants: a review. Microbiol Res 209:21–32

    Article  CAS  Google Scholar 

  • Patel D, Patel A, Vora D, Menon S, Vadakan S, Acharya D, Goswami D (2018) A resourceful methodology to profile indolic auxins produced by rhizo-fungi using spectrophotometry and HPTLC. 3 Biotech 8(10):413

    Article  Google Scholar 

  • Patel D, Patel A, Vora D, Desai K, Menon S, Vadakan S, Goswami D (2019) Profiling indolic auxins produced by the strains of Aspergillus using novel HPTLC technique, Advances in plant & microbial biotechnology. Springer, Singapore, pp 49–58

    Google Scholar 

  • Patel D, Vora S, Gangawane A, Menon S, Goswami D (2020) Talaromyces pinophilus strain M13: a depiction of new pioneering fungal strain for Phytointensification. Biosc Biotech Res Comm 13(1):216–223. https://doi.org/10.21786/bbrc/13.1specialissue/35

  • Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–370

  • Sampaolesi S, Gamba RR, De-Antoni GL, Peláez ÁML (2019) Potentiality of yeasts obtained as beer fermentation residue to be used as probiotics. LWT:108251

  • Sarwar A, Hassan MN, Imran M, Iqbal M, Majeed S, Brader G, Hafeez FY (2018) Biocontrol activity of surfactin A purified from Bacillus NH-100 and NH-217 against rice bakanae disease. Microbiol Res 209:1–13

    Article  CAS  Google Scholar 

  • Sharma V, Salwan R, Shanmugam V (2018) Unraveling the multilevel aspects of least explored plant beneficial Trichoderma saturnisporum isolate GITX-Panog (C). Eur J Plant Pathol 152(1):169–183

    Article  CAS  Google Scholar 

  • Singh D, Raina TK, Kumar A, Singh J, Prasad R (2019a) Plant microbiome: a reservoir of novel genes and metabolites. Plant Gene 18:100177

    Article  CAS  Google Scholar 

  • Singh M, Singh D, Gupta A, Pandey KD, Singh PK, Kumar A (2019b) Plant growth promoting rhizobacteria: application in biofertilizers and biocontrol of phytopathogens, PGPR amelioration in sustainable agriculture. Woodhead Publishing, pp 41–66

  • Singh VK, Singh M, Singh SK, Kumar C, Kumar A (2019c) Sustainable agricultural practices using beneficial fungi under changing climate scenario, Climate change and agricultural ecosystems. Woodhead Publishing, pp 25–42

  • Skiada V, Faccio A, Kavroulakis N, Genre A, Bonfante P, Papadopoulou KK (2019) Colonization of legumes by an endophytic Fusarium solani strain FsK reveals common features to symbionts or pathogens. Fungal Genet Biol 127:60–74

    Article  Google Scholar 

  • Tandon A, Fatima T, Shukla D, Tripathi P, Srivastava S, Singh PC (2019) Phosphate solubilization by Trichoderma koningiopsis (NBRI-PR5) under abiotic stress conditions. J King Saud Univ Sci

  • Teng Y, Du X, Wang T, Mi C, Yu H, Zou L (2018) Isolation of a fungus Pencillium sp. with zinc tolerance and its mechanism of resistance. Arch Microbiol 200(1):159–169

    Article  CAS  Google Scholar 

  • Verma PP, Shelake RM, Das S, Sharma P, Kim JY (2019) Plant growth-promoting rhizobacteria (PGPR) and fungi (PGPF): potential biological control agents of diseases and pests. Microbial Interventions in Agriculture and Environment. Springer, Singapore, p 281-311

  • Yobo KS, Mngadi ZNC, Laing MD (2019) Efficacy of two potassium silicate formulations and two Trichoderma strains on Fusarium head blight of wheat. Proc Natl Acad Sci India Sect B Biol Sci 89(1):185–190

    Article  CAS  Google Scholar 

  • Zheng YK, Qiao XG, Miao CP, Liu K, Chen YW, Xu LH, Zhao LX (2016) Diversity, distribution and biotechnological potential of endophytic fungi. Ann Microbiol 66(2):529–542

    Article  CAS  Google Scholar 

Download references

Funding

Authors are thankful to UGC for providing fund under the F. 30-521/2020(BSR), Under Secretary FD-III Section, University Grant Commission, New Delhi 110002.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Waghodia, Gujarat, 380058, India

    Dhavalkumar Patel

  2. Department of Biological Sciences, P.D. Patel Institute of Applied Sciences, Charusat University, Changa, Gujarat, India

    Abhishek Patel

  3. Department of Biotechnology, School of Science, Royal Melbourne Institute of Technology, Melbourne, Australia

    Mahima Patel

  4. Department of Microbiology and Biotechnology, School of Sciences (SoS), Gujarat University, Ahmedabad, India

    Dweipayan Goswami

Authors
  1. Dhavalkumar Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abhishek Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mahima Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dweipayan Goswami
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

DP and DG conceptualized the work and performed data curation. DP and MP wrote the manuscript. DG conceptualize idea. Review and editing were performed by AP and MP. Supervision was conducted by DG.

Corresponding author

Correspondence to Dweipayan Goswami.

Ethics declarations

Competing interest

The authors declare that they have no conflict of interest.

Ethical approval

Not applicable.

Human and animal rights and informed consent

This article does not contain any studies with human participants or animals performed by any of the authors.

Author approval

All authors have seen and approved the manuscript, and it has not been accepted or published elsewhere.

Consent for publication

All the co-authors consent the publication of this work.

Consent to participate

Not applicable.

Additional information

Responsible Editor: Diane Purchase

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Patel, D., Patel, A., Patel, M. et al. Talaromyces pinophilus strain M13: a portrayal of novel groundbreaking fungal strain for phytointensification. Environ Sci Pollut Res 28, 8758–8769 (2021). https://doi.org/10.1007/s11356-020-11152-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-020-11152-w

Keywords

Search

Navigation