Abstract
In this work, we have prepared cerium oxide (CeO2) nanoparticles (NPs) by laser ablation in water at different laser energies. The structural and optical properties of synthesized nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), Raman spectroscopy, energy dispersive X-ray (EDX), and UV-Vis absorption. XRD results confirmed that the synthesized cerium oxide NPs were crystalline in nature with cubic structure. SEM investigations show that the nanoparticles having a spherical shape with diameter ranged from 26 to 37 nm depending on the laser energy. The antibacterial activity and minimal inhibition concentration of synthesized CeO2 NPs against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were examined. Bacterial adhesion test of cerium oxide NPs was also determined under different incubation temperatures. Cytotoxicity of CeO2 NP effect against the human throat cancer was studied. The cytotoxicity effect of CeO2 NPs synthesized at 160 mJ on the cancer cells caused a free radical releasing which causing oxidative stress. The cytotoxicity effects of ceria NPs against human throat cancer (RD rhabdomyosarcoma cell line) and mouse fibroblast L cell (L20B cell line) growth were 33% and 13%, respectively.
Similar content being viewed by others
References
Alpaslan E, Geilich BM, Yazici H, Thomas J (2017) PH-controlled cerium oxide nanoparticle inhibition of both Gram-positive and Gram-negative bacteria growth. Sci Rep 7:1–12. https://doi.org/10.1038/srep45859
Anandalakshmi K, Venugobal J, Ramasamy V (2015) Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl Nanosci 6:399–408. https://doi.org/10.1007/s13204-015-0449-z
Arumugam A, Karthikeyan C, Hameed ASH, Gopinath K, Gowri S, Karthika V (2015) Synthesis of cerium oxide nanoparticles using Gloriosa superba L. leaf extract and their structural, optical and antibacterial properties. Mater Sci Eng C 4:408–415. https://doi.org/10.1016/j.msec.2015.01.042
Babenko LP, Zholobak NM, Shcherbakov AB, Voychuk SI, Lazarenko LM, Spivak MY (2012) Antibacterial activity of cerium colloids against opportunistic microorganisms in vitro. Mikrobiol Z 74:54–62 УДК 544.773.422:546.05–06 + 579.864.1:615.331
Bellio P et al (2018) Cerium oxide nanoparticles as potential antibiotic adjuvant. Effects of CeO2 nanoparticles on bacterial outer membrane permeability. Biochim Biophys Acta Biomembr 1860:2428–2435. https://doi.org/10.1016/j.bbamem.2018.07.002
Chelliah M, Bosco J, Rayappan B, Krishnan UM (2012) Synthesis and characterization of cerium oxide nanoparticles by hydroxide mediated approach. J Appl Sci 12(16):1734–1737. https://doi.org/10.3923/jas.2012.1734.1737
Chen J, Patil S, Seal S, McGinnis JF (2008) Nanoceria particles prevent ROI-induced blindness. Adv Exp Med Biol 613:53–59. https://doi.org/10.1007/978-0-387-74904-4_5
D’Angelo B, Santucci S, Benedetti E, di Loreto S, Phani RA, Falone S, Amicarelli F, Ceru PM, Cimini A (2009) Cerium oxide nanoparticles trigger neuronal survival in a human Alzheimer disease model by modulating BDNF pathway. Curr Nanosci 5(2):167–176. https://doi.org/10.2174/157341309788185523
Diaconeasa Z, Barbu-Tudoran L, Coman C, Leopold L (2015) Cerium oxide nanoparticles and its cytotoxicity human lung cancer cells. Rom Biotechnol Lett 20(4):10679–10687
Farias IAP, dos Santos CCL, Sampaio FC (2018) Antimicrobial activity of cerium oxide nanoparticles on opportunistic microorganisms: a systematic review. Biomed Res Int 2018:1–14. https://doi.org/10.1155/2018/1923606
Gao Y, Chen K, Ma J, Gao F (2014) Cerium oxide nanoparticles in cancer, Onco Targets Ther 7:835–840. https://doi.org/10.2147/OTT.S62057
Gopinath K, Gowri S, Arumugam A (2013) Phytosynthesis of silver nanoparticles using Pterocarpus santalinus leaf extract and their antibacterial properties. J Nanostructure Chem 3(1):1–7. https://doi.org/10.1186/2193-8865-3-68
Gu J-D, Belay B, Mitchell R (2001) Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins. World J Microbiol Biotechnol 17(2):173–179. https://doi.org/10.1023/A:1016687630330
Gupta A, Das S, Neal CJ, Seal S (2016) Controlling the surface chemistry of cerium oxide nanoparticles for biological applications. J Mater Chem B 4(19):3195–3202. https://doi.org/10.1039/C6TB00396F
Ismail R, Abdul-Hamed R (2017) Laser ablation of Au–CuO core–shell nanocomposite in water for optoelectronic devices. Mater Res Express 4:1–12. https://doi.org/10.1088/2053-1591/aa9e14
Ismail R, Sulaiman G, Marzoog T (2015) Antibacterial activity of magnetic iron oxide nanoparticles synthesized by laser ablation in liquid. Mater Sci Eng C 53:28–297. https://doi.org/10.1016/j.msec.2015.04.047
Ismail R, Sulaiman G, Abdulrahman S (2016) Preparation of iron oxide nanoparticles by laser ablation in DMF under effect of external magnetic field. Int J Mod Phys B 30(17):1650094–16500011. https://doi.org/10.1142/S0217979216500946
Ismail R, Khashan K, Mahdi R (2017) Characterization of high photosensitivity nanostructured 4H-SiC/p-Si heterostructure prepared by laser ablation of silicon in ethanol. Mater Sci Semicond Process 68(12):251–262. https://doi.org/10.1016/j.mssp.2017.06.035
Ismail RA, Sulaiman GM, Mohsin MH, Saadoon AH (2018) Preparation of silver iodide nanoparticles using laser ablation in liquid for antibacterial applications. IET nanobiotechnol 12(6):781–786. https://doi.org/10.1049/iet-nbt.2017.0231
Kaczmarek M (1996) Scanning electron microscopy study of etched capillary surfaces. Master thesis, Faculty of the department of chemistry. San Jose State University. USA
Karakoti A, Singh S, Dowding JM, Seal S, Self WT (2010) Redox-active radical scavenging nanomaterials. Chem Soc Rev 39:4422–4432. https://doi.org/10.1039/B919677N
Khashan KS, Sulaiman GM, Hussain SA, Marzoog TR, Jabir MS (2020) Synthesis, characterization and evaluation of anti-bacterial, anti-parasitic and anti-cancer activities of aluminum-doped zinc oxide nanoparticles. J Inorg Organomet Polym Mater 29:1–7. https://doi.org/10.1007/s10904-020-01522-9
Lee M (2017) A Raman study of CeO2 nanomaterials with different morphologies. Master thesis, Department of Chemical and Biological Engineering, Friedrich-Alexander University Erlangen-Nürnberg
Lerebour G, Cupferman S, Bellon-Fontaine MN (2004) Adhesion of Staphylococcus aureus and Staphylococcus epidermidis to the Episkin reconstructed epidermis model and to an inert 304 stainless steel substrate. J Appl Microbiol 97(1):7–16. https://doi.org/10.1111/j.1365-2672.2004.02181.x
Li Y (2013) Laser ablation in liquid of germanium in extremely electric fields. Master thesis. Faculty of the University of Missouri-Kansas City
Lopez D A (2014) Study of the toxicity of synthetically- and biologically-produced cadmium telluride nanoparticles and an examination of the effects of sadenosyl methionine amendment on bnf 05 bacterial headspace. Master thesis, The Faculty of the Department of Chemistry, Sam Houston State University
Moniri S, Ghoranneviss M, Hanthzadeh MR, Asadabad MA (2016) Synthesis and optical characterization of copper nanoparticles prepared by laser ablation. J Bull Mater Sci 40(1)
Mutinda S I (2013) Hydrothermal synthesis of shape/size-controlled cerium-based oxides. Master thesis, Chemistry department, Youngstown State University
Nelson BC, Johnson ME, Walker ML, Riley KR, Sims CM (2016) Antioxidant cerium oxide nanoparticles in biology and medicine. Antioxidants 5(15)
Otto K, Norbeck J, Larsson T, Karlsson K, Hermansson M (2001) Adhesion of type 1-fimbriated Escherichia coli to abiotic surfaces leads to altered composition of outer membrane proteins. J Bacteriol 183(8):2445–2453. https://doi.org/10.1128/JB.183.8.2445-2453.2001
Patel C, (2009) Room temperature synthesis and systematic characterization of ultra-small ceria nanoparticles. Master thesis, department of chemistry, college of science, University of Central Florida
Philip JT, Koshy CP (2016) Synthesis and characterization of ceria, ceria zircon hybrid and surfactant-modified hybrid nanoparticles for lubricant applications. Int J Adv Prod Mech Eng 2(5):50–58 http://troindia.in/journal/ijapme/editors.html
Phokha S, Pinitsoontorn S, Chirawatkul P, Poo-arporn Y, Maensiri S (2012) Synthesis, characterization, and magnetic properties of monodisperse CeO2 nanospheres prepared by PVP-assisted hydrothermal method. Nanoscale Res Lett 7:425–437. https://doi.org/10.1186/1556-276X-7-425
Reshma P, Ashwini K (2017) Cerium oxide nanoparticles: synthesis, characterization and study of antimicrobial activity. J Nanomater Nanotechno Mole Nanotech 6(3):1–4. https://doi.org/10.4172/2324-8777.1000219
Sebastiammal SS, Shally V, Priyadharshini M, Jayam SG (2017) Structural and optical properties of cerium oxide nanoparticles. Int J Eng Trends Technol 49(2):69–73. https://doi.org/10.14445/22315381/IJETT-V49P211
Shah V, Shah S, Shah H, Rispoli FJ, McDonnell KT, Workeneh S, Karakoti A, Kumar A, Seal S (2012) Antibacterial activity of polymer coated cerium oxide nanoparticles. PLoS One 7(10):1–13. https://doi.org/10.1371/journal.pone.0047827
Silva V, Sousa J, Guerra F, Pessao H, Freitas A, Alves L, Lima E (2015) Antibacterial activity of Ocimum basilicum essential oils and linalool on bacterial isolates of clinical importance. Int J Pharmacognosy and Phytochem 7(6):1066–1071 www.ijppr.com
Singh S (2016) Cerium oxide based nanozymes: redox phenomenon at biointerfaces. Biointerphase 11(4):1–12. https://doi.org/10.1116/1.4966535
Slavin YN, Asnis J, Häfeli UO, Bach H (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J Nanobiotechnol 15(1):65–84. https://doi.org/10.1186/s12951-017-0308-z
Surendra TV, Roopan SM (2016) Photocatalytic and antibacterial properties of phytosynthesized CeO2 NPs using Moringa oleifera peel extract. J Photochem Photobiol B Biol 161:122–128. https://doi.org/10.1016/j.jphotobiol.2016.05.019
Svetlichnyi VA, Lapin IN (2016) Production of CeO2 nanoparticles by method of laser ablation of bulk metallic cerium targets in liquid. Russ Phys J 58:1598–1604. https://doi.org/10.1007/s11182-016-0689-8
Thill A, Zeyons O, Spalla O, Chauvat F, Rose J, Auffan M, Flank AM (2006) Cytotoxicity of CeO2 nanoparticles for Escherichia coli. Physico-chemical insight of the cytotoxicity mechanism. Environ Sci Technol 40(19):6151–6156. https://doi.org/10.1021/es060999b
Tielker D, Hacker S, Loris R, Strathmann M, Wingender J, Wilhelm S, Rosenau F, Jaeger K (2005) Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation. Microbiology 151:1313–1323. https://doi.org/10.1099/mic.0.27701-0
Xu C, Qu X (2014) Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications. NPG Asia Mater 6:1–16. https://doi.org/10.1038/am.2013.88
Yin L, Wang Y, Pang G, Koltypin Y, Gedanken A (2002) Sonochemical synthesis of cerium oxide nanoparticles—effect of additives and quantum size effect. J Colliod Interface Sci 246(1):78–84. https://doi.org/10.1006/jcis.2001.8047
Zamiri R, Ahangar HA, Kaushal A, Zakaria A, Zamiri G, Tobaldi D, Ferreira JM (2015) Dielectrical properties of CeO2 nanoparticles at different temperatures. PLoS One 10(4):1–11. https://doi.org/10.1371/journal.pone.0122989
Zeraik AE, Nitschke M (2012) Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces. Braz Arch Biol Technol 55(4):669–676
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Responsible editor: Lotfi Aleya
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Abid, S.A., Taha, A.A., Ismail, R.A. et al. Antibacterial and cytotoxic activities of cerium oxide nanoparticles prepared by laser ablation in liquid. Environ Sci Pollut Res 27, 30479–30489 (2020). https://doi.org/10.1007/s11356-020-09332-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-020-09332-9