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Radiolabeling of amide functionalized multi-walled carbon nanotubes for bioaccumulation study in fish bone using whole-body autoradiography

  • Multi-Stressors in Freshwater and Transitional Environments: from Legacy Pollutants to Emerging Ones
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Abstract

Commercial and medicinal applications of functionalized carbon nanotubes (f-CNTs) such as amidated f-CNTs are expanding rapidly with a potential risk exposure to living organisms. The effects of amidated f-CNTs on aquatic species have received a limited attention. In this work, an easy wet method to prepare [14C]-label amide multi-walled carbon nanotubes (MWNTs) is reported. Labeled carbon nanotubes were prepared by successive reactions of carboxylation, chloroacylation, and final amidation using [14C]-labeled ethanolamine. The f-CNTs were characterized using elemental analysis, electron dispersive X-ray, transmission electron microscopy, thermogravimetric analysis, and Raman and FTIR spectroscopy. An uptake experiment was carried out with juvenile Arctic char (Salvelinus alpinus) using water dispersed amidated [14C]-f-CNTs to assess their biodistribution in fish tissues using whole body autoradiography. The radioactivity pattern observed in fish head suggests that f-CNTs were accumulated in head bone canals, possibly involving an interaction with mineral or organic phases of bones such as calcium and collagen. This f-CNTs distribution illustrates how important is to consider the surface charges of functionalized carbon nanotubes in ecotoxicological studies.

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Funding

This research work was funded by the Natural Sciences and Engineering Research Council of Canada and supported by the Canada Research Chair in Molecular Ecotoxicology (E.P).

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Authors and Affiliations

  1. Département de biologie, chimie et géographie, Université du Québec à Rimouski, 300, Allée des Ursulines, Rimouski, QC, G5L 3A1, Canada

    Youssouf Djibril Soubaneh

  2. Institut des sciences de la mer de Rimouski, Université du Québec à Rimouski, 310 Allée des Ursulines, Rimouski, QC, G5L 3A1, Canada

    Emilien Pelletier, Isabelle Desbiens & Claude Rouleau

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  1. Youssouf Djibril Soubaneh
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  2. Emilien Pelletier
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  3. Isabelle Desbiens
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  4. Claude Rouleau
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Corresponding author

Correspondence to Youssouf Djibril Soubaneh.

Ethics declarations

This research involved experiments on animals, Arctic char, due to the potential impact of f-CNTs on organisms in aquatic environment. This study was performed in strict accordance with Ethical Policy for Animal Experimentation (Publication No. C2-D34, Rev. 2012) approved by the Institutional Animal Care and Use Committee of the Université du Québec à Rimouski. This ethical policy is an application of the Canadian Council on Animal Care. Post-experimental cares of animals were provided including minimizing discomfort and the consequences of any disability resulting from the experiment.

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The authors declare that they have no conflict of interest (financial or non-financial).

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Soubaneh, Y.D., Pelletier, E., Desbiens, I. et al. Radiolabeling of amide functionalized multi-walled carbon nanotubes for bioaccumulation study in fish bone using whole-body autoradiography. Environ Sci Pollut Res 27, 3756–3767 (2020). https://doi.org/10.1007/s11356-019-05794-8

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  • DOI: https://doi.org/10.1007/s11356-019-05794-8

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