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Bioconversion of lignin into bioplastics by Pandoraea sp. B-6: molecular mechanism

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Abstract

Lignin is a byproduct in the pulp and paper industry and is considered as a promising alternative for the provision of energy and chemicals. Currently, the efficient valorization of lignin is a challenge owing to its polymeric structure complexity. Here, we present a platform for bio-converting Kraft lignin (KL), to polyhydroxyalkanoate (PHA) by Pandoraea sp. B-6 (hereafter B-6). Depolymerization of KL by B-6 was first confirmed, and > 40% KL was degraded by B-6 in the initial 4 days. Characterization of PHA showed that up to 24.7% of PHA accumulated in B-6 grown in 6-g/L KL mineral medium. The composition, structure, and thermal properties of the produced PHA were analyzed, revealing that 3-hydroxybutyrate was the only monomer and that PHA was comparable with the commercially available bioplastics. Moreover, the genomic analysis illustrated three core enzymatic systems for lignin depolymerization including laccases, peroxidases, and Fenton-reaction enzymes; five catabolic pathways for LDAC degradation and a gene cluster consisting of bktB, phaR, phaB, phaA, and phaC genes involved in PHA biosynthesis. Accordingly, a basic model for the process from lignin depolymerization to PHA production was constructed. Our findings provide a comprehensive perspective for lignin valorization and bio-material production from waste.

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Funding

This work was supported by the National Key R&D Plan (2016YFC0403003), the National Natural Science Foundation of China (31400115, 51474102), and the China Postdoctoral Science Foundation (2017M612594).

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Authors and Affiliations

  1. Institute of Environmental Science and Engineering, School of Metallurgy and Environment, Central South University, Changsha, 410083, China

    Dan Liu, Xu Yan, Mengying Si, Xinhui Deng, Xiaobo Min, Yan Shi & Liyuan Chai

  2. Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, Changsha, 410083, China

    Xu Yan, Xiaobo Min, Yan Shi & Liyuan Chai

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  1. Dan Liu
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  2. Xu Yan
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  3. Mengying Si
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  4. Xinhui Deng
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  5. Xiaobo Min
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  6. Yan Shi
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  7. Liyuan Chai
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Correspondence to Yan Shi or Liyuan Chai.

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Liu, D., Yan, X., Si, M. et al. Bioconversion of lignin into bioplastics by Pandoraea sp. B-6: molecular mechanism. Environ Sci Pollut Res 26, 2761–2770 (2019). https://doi.org/10.1007/s11356-018-3785-1

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