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Anxiety and memory deficits induced by tannery effluent in C57BL/6J female mice

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Abstract

This study aimed to evaluate the behavior of female C57Bl/6J mice exposed to tannery effluents diluted in drinking water. Female mice were divided into a control group, in which the animals received only drinking water, and experimental groups, which received raw tannery effluent in 7.5 and 15 % concentrations diluted in water (period of 60 days). In the last experimental week, the mice (in diestrus phase) were subjected to different behavioral tests: elevated plus-maze, open-field test, forced swim test, and object recognition test. Our data demonstrated that exposure to tannery effluent increased the anxiety index of animals and decreased the locomotion ratio in the central quadrants/total, indicating an increase in anxiety-like behavior. Regarding the forced swim test, we did not observe changes in the evaluated behaviors. There were no statistically significant differences in the recognition index of the novel and familiar object in the groups exposed to tannery effluent compared with the control group, indicating a possible influence of the constituents of tannery effluent on cognition. Thus, our findings support the hypothesis that effluents, containing neurotoxic substances, could cause behavioral disruptions in female C57Bl/6J mice.

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Authors and Affiliations

  1. Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil

    Abraão Tiago Batista Guimarães, Raissa de Oliveira Ferreira, Joyce Moreira de Souza & Wellington Alves Mizael da Silva

  2. Laboratório de Estatística Experimental, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil

    Anderson Rodrigo da Silva

  3. Departamento de Ciências Biológicas, Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil

    Aline Sueli de LimaRodrigues

  4. Departamento de Biologia Geral and Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás, Câmpus Samambaia, Goiânia, Brazil

    Daniela de Melo e Silva

  5. Departamento de Farmacologia and Programa de Pós-Graduação em Biodiversidade Universidade Federal de Goiás, Câmpus Samambaia, Goiânia, Brazil

    Renata Mazaro e Costa

  6. Departamento de Ciências Biológicas. Laboratório de Zoologia, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil

    André Luis da Silva Castro

  7. Departamento de Ciências Biológicas, Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil

    Guilherme Malafaia

  8. Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás—Câmpus Samambaia, Goiânia, Brazil

    Guilherme Malafaia

  9. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, CEP: 75790-000, Brazil

    Guilherme Malafaia

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  1. Abraão Tiago Batista Guimarães
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  2. Raissa de Oliveira Ferreira
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  6. Aline Sueli de LimaRodrigues
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  7. Daniela de Melo e Silva
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Correspondence to Guilherme Malafaia.

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Responsible editor: Philippe Garrigues

Main finding of the work:

The exposure to tannery effluent causes behavioral disruptions in female C57Bl/6J mice. We observed an anxiogenic effect in female mice, with short-term and long-term impaired memory

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Guimarães, A.T.B., de Oliveira Ferreira, R., de Souza, J.M. et al. Anxiety and memory deficits induced by tannery effluent in C57BL/6J female mice. Environ Sci Pollut Res 23, 25323–25334 (2016). https://doi.org/10.1007/s11356-016-7746-2

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