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Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells

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Abstract

Exposure to coal and coal ashes can cause harmful effects in in vitro and in vivo systems, mainly by the induction of oxidative damage. The aim of this work was to assess cytotoxic and genotoxic effects using the V79 cell line treated with coal and coal fly ash particles derived from a coal power plant located in Santa Catarina, Brazil. Two coal samples (COAL11 and COAL16) and two coal fly ash samples (CFA11 and CFA16) were included in this study. COAL16 was co-firing with a mixture of fuel oil and diesel oil. The comet assay data showed that exposure of V79 cells to coal and coal fly ash particles induced primary DNA lesions. Application of lesion-specific endonucleases (FPG and ENDO III) demonstrated increased DNA effects indicating the presence of high amounts of oxidative DNA lesions. The cytokinesis-block micronucleus cytome assay analysis showed that exposure of V79 cells to high concentrations of coal and coal fly ash particles induced cytotoxic effects (apoptosis and necrosis) and chromosomal instability (nucleoplasmic bridges, nuclear buds, and micronucleus (MN) formation). These results may be associated with compounds contained in the surface of the particles as hazardous elements, ultrafine/nanoparticles, and polycyclic aromatic hydrocarbons (PAHs) which were detected in the samples.

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Acknowledgments

This work was supported by grants from Conselho Nacional para o Desenvolvimento Científico e Tecnológico—CNPq, Brazil; Universal Grant Number 470833; Productivity Grant Numbers 300902 and 303364; and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Brazil, Grant Number PRONEM 11/2070 and PRONEX/FAPERGS/CNPq Number 10/0044-3). Grethel León-Mejía benefited from a scholarship financed by PEC-PG/CAPES. Marcos L.S. Oliveira and Luis F.O. Silva benefited from a scholarship financed by CNPq.

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Authors and Affiliations

  1. Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

    Grethel León-Mejía & João Antônio Pêgas Henriques

  2. Unidad de Investigación, Desarrollo e Innovación en Genética y Biología Molecular, Universidad Simón Bolívar, Barranquilla, Colombia

    Grethel León-Mejía

  3. Research group in Environmental Management and Sustainability, Faculty of Environmental Sciences, Universidad de la Costa, Barranquilla, Colombia

    Luis F. O. Silva

  4. Universidade do Sul de Santa Catarina, Pró-Reitoria de Ensino, de Pesquisa e de Extensão, UNISUL -Universidade do Sul de Santa Catarina Pedra Branca, Palhoça, SC, 88137900, Brazil

    Luis F. O. Silva, Matheus S. Civeira & Marcos L. S. Oliveira

  5. InnVitro Pesquisa e Desenvolvimento, Porto Alegre, RS, Brazil

    Miriana Machado & Izabel Vianna Villela

  6. Novartis Pharma AG, Basel, Switzerland

    Andreas Hartmann

  7. Laboratório de Genética Toxicológica, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil

    Suziane Premoli, Dione Silva Corrêa & Juliana Da Silva

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  1. Grethel León-Mejía
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  2. Luis F. O. Silva
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  4. Marcos L. S. Oliveira
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  11. João Antônio Pêgas Henriques
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Correspondence to Grethel León-Mejía or João Antônio Pêgas Henriques.

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León-Mejía, G., Silva, L.F.O., Civeira, M.S. et al. Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells. Environ Sci Pollut Res 23, 24019–24031 (2016). https://doi.org/10.1007/s11356-016-7623-z

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