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Microbial degradation of endosulfan in contaminated soil with the elution of surfactants

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Abstract

In this work, an endosulfan-degrading strain was isolated from the aged soil contaminated by endosulfan, and identified as Ochrobactrum sp. EB-4 by 16S rDNA sequence analysis. The microbial degradation characteristics of endosulfan in three eluents (Tween 80 + SDS, Tween 80 + Na2SiO3, Tween 80 + SDS + Na2SiO3) were investigated. The results showed that the degradation percents of α-, β-endosulfan in the three eluents were 86.83 %∼92.91 % and 88.90 %∼93.94 % in 15 days, respectively. The degradation process can be well described by the first-order kinetic model, and the half-times of α-endosulfan in eluent 1∼eluent 3 were 3.83, 5.29, and 4.53 days, while those of β-endosulfan were 3.35, 4.50 and 3.79 days, respectively. The endosulfan diol and endosulfan sulfate as main metabolites were detected, and the former can be further degraded by this strain, which revealed that the simultaneously happened hydrolysis and oxidation reactions were the main degradation processes, and dominated by hydrolysis reaction. After 5 days of washing with the eluents, 56.00∼84.33 % of α-endosulfan, and 46.49∼68.56 % of β-endosulfan in soil were eluted, respectively, and can be entirely biodegraded in 12 days, which indicated that the microbial degradation was the rate-determining step.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (No. 2012AA101405) and the National Public Service Sectors (Agriculture) Research Project of China (No. 2009030).

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Authors and Affiliations

  1. College of Resources and Environment, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Southwest University, Chongqing, 400715, China

    Fei Deng, Bailian Xiong & Jinzhong Zhang

  2. Department of Resources and Environment, Zunyi Normal College, Zunyi, Guizhou, 563002, People’s Republic of China

    Bailian Xiong

  3. Chongqing Chemical Engineering Vocational College, Chongqing, 400020, China

    Benshou Chen

  4. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing, 400020, People’s Republic of China

    Guocan Zheng

  5. Chongqing Key Laboratory of Agricultural Resources and Environment, Chongqing, 400716, People’s Republic of China

    Jinzhong Zhang

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  1. Fei Deng
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  2. Bailian Xiong
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Correspondence to Jinzhong Zhang.

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Deng, F., Xiong, B., Chen, B. et al. Microbial degradation of endosulfan in contaminated soil with the elution of surfactants. Environ Sci Pollut Res 23, 13268–13275 (2016). https://doi.org/10.1007/s11356-016-6523-6

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