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Enhanced nutrient removal from municipal wastewater assisted by mixotrophic microalgal cultivation using glycerol

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Abstract

In a present study, nutrient removal from municipal wastewater by Chlorella vulgaris and Nannochloropsis oculata was investigated by using mixotrophic cultivation with glycerol (0 to 5 g/L). Performance parameters were assessed by estimating the removal of total nitrogen, total phosphorus, chemical oxygen demand (COD), biomass growth, chlorophyll content, lipid yield, and fatty acids. With the addition of 2 g/L glycerol, a maximum biomass productivity of 56 mg/L/day was achieved in the mixotrophic culture of C. vulgaris within 12 days. The mixotrophic culture showed a 30-fold increase in biomass productivity compared to the wastewater without any glycerol. However, the highest total nitrogen removal (80.62 %), total phosphate removal (60.72 %), and COD removal (96.3 %) was observed in the N. oculata culture supplemented with 3, 5, and 1 g/L glycerol, respectively. These results suggest that mixotrophic cultivation using glycerol offers great potential in the production of renewable biomass, waste water treatment, and consequent production of high-value microalgal oil.

Simultaneous biomass production and nutrient removal using microalgae cultivated in wastewater supplemented with glycerol

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Acknowledgments

This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Korean Government (MEST) (2012R1A2A4A01001539).

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  1. Department of Energy and Environment Convergence, Catholic Kwandong University, Gangneung, 210701, South Korea

    Prabuddha L. Gupta, Hee Jeong Choi & Seung-Mok Lee

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  1. Prabuddha L. Gupta
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  2. Hee Jeong Choi
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Correspondence to Seung-Mok Lee.

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Gupta, P.L., Choi, H.J. & Lee, SM. Enhanced nutrient removal from municipal wastewater assisted by mixotrophic microalgal cultivation using glycerol. Environ Sci Pollut Res 23, 10114–10123 (2016). https://doi.org/10.1007/s11356-016-6224-1

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