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Characterization of H2S removal and microbial community in landfill cover soils

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Abstract

H2S is a source of odors at landfills and poses a threat to the surrounding environment and public health. In this work, compared with a usual landfill cover soil (LCS), H2S removal and biotransformation were characterized in waste biocover soil (WBS), an alternative landfill cover material. With the input of landfill gas (LFG), the gas concentrations of CH4, CO2, O2, and H2S, microbial community and activity in landfill covers changed with time. Compared with LCS, lower CH4 and H2S concentrations were detected in the WBS. The potential sulfur-oxidizing rate and sulfate-reducing rate as well as the contents of acid-volatile sulfide, SO4 2−, and total sulfur in the WBS and LCS were all increased with the input of LFG. After exposure to LFG for 35 days, the sulfur-oxidizing rate of the bottom layer of the WBS reached 82.5 μmol g dry weight (d.w.)−1 day−1, which was 4.3-5.4 times of that of LCS. H2S-S was mainly deposited in the soil covers, while it escaped from landfills to the atmosphere. The adsorption, absorption, and biotransformation of H2S could lead to the decrease in the pH values of landfill covers; especially, in the LCS with low pH buffer capacity, the pH value of the bottom layer dropped to below 4. Pyrosequencing of 16S ribosomal RNA (rRNA) gene showed that the known sulfur-metabolizing bacteria Ochrobactrum, Paracoccus, Comamonas, Pseudomonas, and Acinetobacter dominated in the WBS and LCS. Among them, Comamonas and Acinetobacter might play an important role in the metabolism of H2S in the WBS. These findings are helpful to understand sulfur bioconversion process in landfill covers and to develop techniques for controlling odor pollution at landfills.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China with Grant No. 41001148, No. 51178411, and No.41371012 and Zhejiang Province Natural Science Foundation for Distinguished Young Scholars (LR13E080002).

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Authors and Affiliations

  1. Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China

    Fang-Fang Xia, Hong-Tao Zhang, Xiao-Meng Wei, Yao Su & Ruo He

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  1. Fang-Fang Xia
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  3. Xiao-Meng Wei
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Correspondence to Ruo He.

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Responsible editor: Zhihong Xu

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Xia, FF., Zhang, HT., Wei, XM. et al. Characterization of H2S removal and microbial community in landfill cover soils. Environ Sci Pollut Res 22, 18906–18917 (2015). https://doi.org/10.1007/s11356-015-5070-x

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