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Hartig’ net formation of Tricholoma vaccinum-spruce ectomycorrhiza in hydroponic cultures

  • Alteration and element mobility at the microbe-mineral interface
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Abstract

For re-forestation of metal-contaminated land, ectomycorrhizal trees may provide a solution. Hence, the study of the interaction is necessary to allow for comprehensive understanding of the mutually symbiotic features. On a structural level, hyphal mantle and the Hartig’ net formed in the root apoplast are essential for plant protection and mycorrhizal functioning. As a model, we used the basidiomycete Tricholoma vaccinum and its host spruce (Picea abies). Using an optimized hydroponic cultivation system, both features could be visualized and lower stress response of the tree was obtained in non-challenged cultivation. Larger spaces in the apoplasts could be shown with high statistical significance. The easy accessibility will allow to address metal stress or molecular responses in both partners. Additionally, the proposed cultivation system will enable for other experimental applications like addressing flooding, biological interactions with helper bacteria, chemical signaling, or other biotic or abiotic challenges relevant in the natural habitat.

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Acknowledgments

This research was funded by the University of Jena and the Research Training Group “Alteration and element mobility at microbe-mineral interfaces". We would like to thank the Graduate School “Jena School for Microbial Communication” (JSMC) as part of the German Excellence Initiative and the German National Academic Foundation for support in this work. The authors especially thank Annekatrin Voigt for technical support.

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The authors state that no potential conflicts of interests exist.

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Authors and Affiliations

  1. Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07734, Jena, Germany

    Catarina Henke, Elke-Martina Jung & Erika Kothe

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  1. Catarina Henke
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  2. Elke-Martina Jung
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  3. Erika Kothe
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Correspondence to Catarina Henke.

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Henke, C., Jung, EM. & Kothe, E. Hartig’ net formation of Tricholoma vaccinum-spruce ectomycorrhiza in hydroponic cultures. Environ Sci Pollut Res 22, 19394–19399 (2015). https://doi.org/10.1007/s11356-015-4354-5

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  • DOI: https://doi.org/10.1007/s11356-015-4354-5

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