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Positive effects of salicylic acid pretreatment on the composition of flax plastidial membrane lipids under cadmium stress

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Abstract

Interest in use of flax (Linum usitatissimum L.) as cadmium (Cd)-accumulating plant for phytoextraction of contaminated soils opened up a new and promising avenue toward improving tolerance of its varieties and cultivars to Cd stress. The aim of this study is to get insights into the mechanisms of Cd detoxification in cell membranes, by exploring the effects of salicylic acid (SA)-induced priming on fatty acids and lipid composition of flax plantlets, grown for 10 days with 50 and 100 μM Cd. At leaf level, levels of monogalactosyldiacylglycerol (MGDG), phosphatidylcholine (PC), phosphatidylglycerol (PG), and neutral lipids (NL) have shifted significantly in flax plantlets exposed to toxic CdCl2 concentrations, as compared to that of the control. At 100 μM Cd, the linoleic acid (C18:2) decreases mainly in digalactosyldiacylglycerol (DGDG) and all phospholipid species, while linolenic acid (C18:3) declines mostly in MGDG and NL. Conversely, at the highest concentration of the metal, SA significantly enhances the levels of MGDG, PG and phosphatidic acid (PA), and polyunsaturated fatty acids mainly C18:2 and C18:3. Furthermore, SA pretreatment seems to reduce the Cd-induced alterations in both plastidial and extraplastidial lipid classes, but preferentially preserves the plastidial lipids by acquiring higher levels of polyunsaturated fatty acids. These results suggest that flax plantlets pretreated with SA exhibits more stability of their membranes under Cd-stress conditions.

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Acknowledgments

This research was supported by the Ministry of Higher Education, Scientific Research and Technology in Tunisia.

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Authors and Affiliations

  1. Département de Biologie, Unité de Recherche de Physiologie et Biochimie de la tolérance des plantes aux contraintes abiotiques, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia

    Aïcha Belkadhi, Wided Chaïbi & Wahbi Djebali

  2. Department of Agronomy and Plant Breeding, Institute of Sustainable Agriculture, Spanish Council for Scientific Research (CSIC), Alameda del Obispo s/n, 14080, Córdoba, Spain

    Antonio De Haro & Sara Obregon

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  1. Aïcha Belkadhi
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  2. Antonio De Haro
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  3. Sara Obregon
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  5. Wahbi Djebali
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Correspondence to Wahbi Djebali.

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Belkadhi, A., De Haro, A., Obregon, S. et al. Positive effects of salicylic acid pretreatment on the composition of flax plastidial membrane lipids under cadmium stress. Environ Sci Pollut Res 22, 1457–1467 (2015). https://doi.org/10.1007/s11356-014-3475-6

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