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Medium optimization for the production of anti-cyanobacterial substances by Streptomyces sp. HJC-D1 using response surface methodology

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Abstract

Bioremediation using isolated anti-cyanobacterial microorganism has been widely applied in harmful algal blooms (HABs) control. In order to improve the secretion of activated anti-cyanobacterial substances, and lower the cost, a sequential optimization of the culture medium based on statistical design was employed for enhancing the anti-cyanobacterial substances production and chlorophyll a (Chl a) removal by Streptomyces sp. HJC-D1 in the paper. Sucrose and KNO3 were selected as the most suitable carbon and nitrogen sources based on the one-at-a-time strategy method, and sucrose, KNO3 and initial pH were found as major factors that affected the anti-cyanobacterial ability of the isolated stain via the Plackett–Burman design. Based on the response surface and canonical analysis, the optimum condition of culture medium was obtained at 22.73 g l-1 of sucrose, 0.96 g l-1 of KNO3, and initial pH 8.82, and the Chl a removal efficiency by strain HJC-D1 increased from 63 ± 2 % to 78 ± 2 % on the optimum conditions.

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Acknowledgments

This work was financially supported by the National Key Technology R&D Program (Nos. 2006BAJ08B01/2012BAJ25B07), the National Key Science and Technology Project: Water Pollution Control and Treatment (No. 2012ZX07101-012).

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Authors and Affiliations

  1. Department of Environmental Engineering, Zhejiang University, Zhejiang, Hangzhou, 310058, China

    Yun Kong, Jiaoqin Qi, Liang Zhu & Xiangyang Xu

  2. Hubei Longyin Environmental Protection Science and Technology, Hubei, Chibi, 437300, China

    Pei Zou

  3. Yixing Urban Supervision and Inspection Administration of Product Quality National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu, Yixing, 214205, China

    Yun Kong, Jiaoqin Qi & Liming Song

  4. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

    Lihong Miao

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  1. Yun Kong
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Correspondence to Liang Zhu or Xiangyang Xu.

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Kong, Y., Zou, P., Miao, L. et al. Medium optimization for the production of anti-cyanobacterial substances by Streptomyces sp. HJC-D1 using response surface methodology. Environ Sci Pollut Res 21, 5983–5990 (2014). https://doi.org/10.1007/s11356-014-2532-5

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  • DOI: https://doi.org/10.1007/s11356-014-2532-5

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