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Gemfibrozil modulates cytochrome P450 and peroxisome proliferation-inducible enzymes in the liver of the yellow European eel (Anguilla anguilla)

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Abstract

The human lipid regulator gemfibrozil (GEM) has been shown to induce peroxisome proliferation in rodents leading to hepatocarcinogenesis. Since GEM is found at biological active concentrations in the aquatic environment, the present study investigates the effects of this drug on the yellow European eel (Anguilla anguilla). Eels were injected with different concentrations of GEM (0.1 to 200 μg/g) and sampled 24- and 96-h post-injection. GEM was shown to inhibit CYP1A, CYP3A and CYP2K-like catalytic activities 24-h post-injection, but at 96-h post-injection, only CYP1A was significantly altered in fish injected with the highest GEM dose. On the contrary, GEM had little effect on the phase II enzymes examined (UDP-glucuronyltransferase and glutathione-S-transferase). Peroxisome proliferation inducible enzymes (liver peroxisomal acyl-CoA oxidase and catalase) were very weakly induced. No evidence of a significant effect on the endocrine system of eels was observed in terms of plasmatic steroid levels or testosterone esterification in the liver.

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Acknowledgements

This study was supported by the Spanish Ministry of Science and Education under Project ref. CGL2005-02846. The authors would like to express their gratitude to the IRTA-SCR technicians (G. Macià and J.L. Celades) for rearing fish for experimental purposes.

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Authors and Affiliations

  1. Environmental Chemistry Department, IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain

    Angeliki Lyssimachou, Rémi Thibaut & Cinta Porte

  2. IRTA, Centre de Sant Carles de la Ràpita, Unitat Cultius Experimentals, 43540 Sant Carles de la Ràpita, Tarragona, Spain

    Enric Gisbert

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  1. Angeliki Lyssimachou
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  2. Rémi Thibaut
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  4. Cinta Porte
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Correspondence to Cinta Porte.

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Lyssimachou, A., Thibaut, R., Gisbert, E. et al. Gemfibrozil modulates cytochrome P450 and peroxisome proliferation-inducible enzymes in the liver of the yellow European eel (Anguilla anguilla). Environ Sci Pollut Res 21, 862–871 (2014). https://doi.org/10.1007/s11356-013-1944-y

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