Abstract
Purpose
[18F]ML-10 is the most advanced radiopharmaceutical for the clinical imaging of the apoptosis phenomenon by PET. The preparation of this radiopharmaceutical on a commercial radiosynthesis module and the requested quality controls for its release are presented herein.
Procedures
ML-10 as reference and its mesyloxy derivative as precursor for labelling with fluorine-18 were prepared. [18F]ML-10 was synthesized via a [18F]fluorine-de-mesyloxy aliphatic nucleophilic substitution via a GE TRACERLab® FX-FN module. Quality controls were performed.
Results
The labelling precursor was obtained in a four step synthesis in 28 % overall yield affording ML-10 in two steps (88 % yield). Pure [18F]ML-10 was obtained with a decay corrected yield of 39.8 % ± 8.4 % (n = 7) in 70 min and a specific activity of 235 ± 85 GBq/μmol at the end of synthesis.
Conclusions
[18F]ML-10 was prepared on a widely available automated module and passed the quality control. A LC/MS method was developed to measure specific radioactivity.
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Acknowledgements
We thank O. Tirel for performing the cyclotron irradiation. This work was supported by the “Région de Basse-Normandie” and the “Commissariat à l’énergie atomique et aux énergies alternatives” (CEA).
Conflict of Interest
The authors declare that they have no conflict of interest.
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Sobrio, F., Médoc, M., Martial, L. et al. Automated Radiosynthesis of [18F]ML-10, a PET Radiotracer Dedicated to Apoptosis Imaging, on a TRACERLab FX-FN Module. Mol Imaging Biol 15, 12–18 (2013). https://doi.org/10.1007/s11307-012-0574-y
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DOI: https://doi.org/10.1007/s11307-012-0574-y