Abstract
Purpose
To investigate the overexpression of miR-221 in papillary thyroid carcinoma (PTC), we developed a Gaussia luciferase (Gluc) system regulated by miR-221.
Procedures
Quantities of primary or mature miR-221 in normal thyroid cells (HT-ori3) and in PTC (NPA, TPC-1) were measured by quantitative real-time polymerase chain reaction. Cytomegalovirus (CMV)/Gluc-3xPT_miR221, which included three perfect complementary target sequences repeats of miR221 in the 3′-untranslated region of Gluc, was transfected into cells with pre-miR-221 or anti-miR-221 and Gluc activities were then compared in vitro and in vivo.
Results
Primary or mature miR-221 were overexpressed in PTC as compared with HT-ori3. In cells transfected with the Gaussia luciferase reporter system (CMV/Gluc-3xPT_miR221), Gluc activities were regulated according to miR-221 levels in vitro and in vivo.
Conclusions
These results suggest that the devised CMV/Gluc-3xPT_miR221 system may be a useful tool for monitoring quantities of endogenous miR-221 in cells or living organisms.
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Acknowledgements
This work was funded by the Korean Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST; No. 2007-02242), National R&D Program for Cancer Control of Ministry of Health & Welfare (0820320), and Seoul R&BD program. H.J. Kim was supported by the BK21 Project for Medicine, Dentistry, and Pharmacy in Korea (2007).
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Kim, H.J., Chung, JK., Hwang, D.W. et al. In Vivo Imaging of miR-221 Biogenesis in Papillary Thyroid Carcinoma. Mol Imaging Biol 11, 71–78 (2009). https://doi.org/10.1007/s11307-008-0188-6
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DOI: https://doi.org/10.1007/s11307-008-0188-6